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CLC number: R711.74

On-line Access: 2017-03-08

Received: 2016-06-30

Revision Accepted: 2016-11-24

Crosschecked: 2017-02-08

Cited: 2

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Citations:  Bibtex RefMan EndNote GB/T7714


Ye-li Yao


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.3 P.256-262


Human papillomavirus (HPV) E6/E7 mRNA detection in cervical exfoliated cells: a potential triage for HPV-positive women

Author(s):  Ye-li Yao, Qi-fang Tian, Bei Cheng, Yi-fan Cheng, Jing Ye, Wei-guo Lu

Affiliation(s):  Department of Gynecologic Oncology, Womens Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China

Corresponding email(s):   yaoyeli1114@163.com, lbwg@zju.edu.cn

Key Words:  Human papillomavirus (HPV), HPV E6/E7 mRNA, High-grade squamous intraepithelial lesion (HSIL)

Ye-li Yao, Qi-fang Tian, Bei Cheng, Yi-fan Cheng, Jing Ye, Wei-guo Lu. Human papillomavirus (HPV) E6/E7 mRNA detection in cervical exfoliated cells: a potential triage for HPV-positive women[J]. Journal of Zhejiang University Science B, 2017, 18(3): 256-262.

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author="Ye-li Yao, Qi-fang Tian, Bei Cheng, Yi-fan Cheng, Jing Ye, Wei-guo Lu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Human papillomavirus (HPV) E6/E7 mRNA detection in cervical exfoliated cells: a potential triage for HPV-positive women
%A Ye-li Yao
%A Qi-fang Tian
%A Bei Cheng
%A Yi-fan Cheng
%A Jing Ye
%A Wei-guo Lu
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 3
%P 256-262
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600288

T1 - Human papillomavirus (HPV) E6/E7 mRNA detection in cervical exfoliated cells: a potential triage for HPV-positive women
A1 - Ye-li Yao
A1 - Qi-fang Tian
A1 - Bei Cheng
A1 - Yi-fan Cheng
A1 - Jing Ye
A1 - Wei-guo Lu
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 3
SP - 256
EP - 262
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600288

Cytology triage has been generally recommended for human papillomavirus (HPV)-positive women, but is highly dependent on well-trained cytologists. The present study was designed to explore whether HPV E6/E7 mRNA detection in cervical exfoliated cells can be a potential triage for HPV-positive women from a clinic-based population. Both the primary HPV testing and Papanicolaou (Pap) test were performed on all eligible HPV-positive women. HPV E6/E7 mRNA was detected by QuantiVirus® HPV E6/E7 mRNA assay in cervical exfoliated cells. All HPV-positive women underwent colposcopy and further biopsy if indicated. The data were assessed by Pearson’s Chi-squared test and the receiver operating characteristic curve. A total of 404 eligible HPV-positive women were enrolled. Positive rate of E6/E7 mRNA in high-grade squamous intraepithelial lesion (HSIL) cases was higher than that in low-grade squamous intraepithelial lesion (LSIL) or normal cases. There was no statistical difference found between mRNA and cytological testing with sensitivity (89.52% vs. 86.67%, P=0.671), specificity (48.96% vs. 48.96%, P=1.000), positive predictive value (39.00% vs. 38.24%, P=1.000), and negative predictive value (92.76% vs. 90.97%, P=0.678) for detecting ≥HSIL. HPV E6/E7 mRNA detection in cervical exfoliated cells shows the same performance as Pap triage for HSIL identification for HPV-positive women. Detection of HPV E6/E7 mRNA may be used as a new triage option for HPV-positive women.

宫颈脱落细胞人乳头瘤病毒(HPV)E6/E7 mRNA检测在HPV阳性女性筛查中的应用

目的:基于临床样本探讨宫颈脱落细胞HPV E6/E7 mRNA检测能否作为HPV阳性女性的筛查手段。
创新点:首次比较了宫颈脱落细胞HPV E6/E7 mRNA检测与宫颈脱落细胞学检测对于HPV阳性女性宫颈活检结果的预测价值。
方法:所有入组的HPV阳性女性均进行宫颈脱落细胞学及HPV E6/E7 mRNA检测。比较两种方法对于宫颈活检结果的预测价值。
结论:对于HPV阳性的女性,高级别鳞状上皮内病变(HSIL)HPV E6/E7 mRNA阳性率高于低级别鳞状上皮内病变(LSIL)及正常病例。HPV E6/E7 mRNA与细胞学检测相比,HSIL及以上级别病变的检测敏感性(89.52% vs. 86.67%,P=0.671)、特异性(48.96% vs. 48.96%,P=1.000)、阳性预测值(39.00% vs. 38.24%,P=1.000)及阴性预测值(92.76% vs. 90.97%,P=0.678)均无显著差异。HPV E6/E7 mRNA检测或能成为HPV阳性女性的一种新的筛查手段。

关键词:人乳头瘤病毒(HPV);HPV E6/E7 mRNA;高级别鳞状上皮内病变(HSIL)

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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