CLC number: R605.971
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2017-04-10
Cited: 0
Clicked: 4532
Feng Yao, Yuan-qiang Lu, Jiu-kun Jiang, Lin-hui Gu, Han-zhou Mou. Immune recovery after fluid resuscitation in rats with severe hemorrhagic shock[J]. Journal of Zhejiang University Science B, 2017, 18(5): 402-409.
@article{title="Immune recovery after fluid resuscitation in rats with severe hemorrhagic shock",
author="Feng Yao, Yuan-qiang Lu, Jiu-kun Jiang, Lin-hui Gu, Han-zhou Mou",
journal="Journal of Zhejiang University Science B",
volume="18",
number="5",
pages="402-409",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600370"
}
%0 Journal Article
%T Immune recovery after fluid resuscitation in rats with severe hemorrhagic shock
%A Feng Yao
%A Yuan-qiang Lu
%A Jiu-kun Jiang
%A Lin-hui Gu
%A Han-zhou Mou
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 5
%P 402-409
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600370
TY - JOUR
T1 - Immune recovery after fluid resuscitation in rats with severe hemorrhagic shock
A1 - Feng Yao
A1 - Yuan-qiang Lu
A1 - Jiu-kun Jiang
A1 - Lin-hui Gu
A1 - Han-zhou Mou
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 5
SP - 402
EP - 409
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600370
Abstract: Objective: To investigate the effects of resuscitation with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES) on regulatory T cells (Tregs), helper T 1 (Th1)/Th2 and cytotoxic T 1 (Tc1)/Tc2 profiles in the treatment of hemorrhagic shock. Methods: Rats subjected to severe hemorrhagic shock were resuscitated for 30 min with NS (n=8), HTS (n=8), or HES (n=8); sham (n=8) and naive control (n=8) groups were used for comparison. Following fluid resuscitation, the whole shed blood was reinfused for 30 min, and the rats were observed with continuous hemodynamic monitoring for 120 min. CD4+CD25+Foxp3+ Treg proportions, Th1/Th2 and Tc1/Tc2 profiles in spleen were analyzed by three-color flow cytometry. Results: The proportion of CD4+CD25+Foxp3+ Tregs and ratios of Th1/Th2 and Tc1/Tc2 did not differ among control, sham, and HTS groups, but were significantly lower in NS and HES groups (both P<0.05 vs. sham); NS and HES levels were similar. The level of Tc1 was significantly increased in HTS (P<0.05 vs. sham), and levels of Tc2 were increased in NS, HES, and HTS groups compared to sham (all P<0.05), but did not differ from each other. Conclusions: HTS resuscitation has a greater impact on immune system recovery than NS or HES by preserving the proportion of Tregs and maintaining the balance between Th1/Th2 and Tc1/Tc2 cells in the spleen. Thus, HTS resuscitation provides potential immunomodulatory activity in the early stage after hemorrhagic shock.
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