Full Text:   <802>

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CLC number: S852.65

On-line Access: 2017-06-05

Received: 2016-09-22

Revision Accepted: 2016-12-16

Crosschecked: 2017-05-10

Cited: 0

Clicked: 1730

Citations:  Bibtex RefMan EndNote GB/T7714


Jiang-bing Shuai


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.6 P.549-554


Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China

Author(s):  Jiang-bing Shuai, Lu-huan Li, Ai-yun Li, Yong-qiang He, Xiao-feng Zhang

Affiliation(s):  Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou 310016, China; more

Corresponding email(s):   zxf@zju.edu.cn

Key Words:  Swine hepatitis E virus, Full genome sequence, Phylogenetic analysis, Genotype 3

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Jiang-bing Shuai, Lu-huan Li, Ai-yun Li, Yong-qiang He, Xiao-feng Zhang. Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China[J]. Journal of Zhejiang University Science B, 2017, 18(6): 549-554.

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publisher="Zhejiang University Press & Springer",

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%T Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China
%A Jiang-bing Shuai
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%A Ai-yun Li
%A Yong-qiang He
%A Xiao-feng Zhang
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%DOI 10.1631/jzus.B1600419

T1 - Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China
A1 - Jiang-bing Shuai
A1 - Lu-huan Li
A1 - Ai-yun Li
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A1 - Xiao-feng Zhang
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Hepatitis E is believed to occur in both endemic and sporadic forms in developing countries, which causes a major public health problem in Asia and Africa (Meng, 2010; Wang et al., 2016a). Recent studies have documented that the disease is also endemic in many industrialized countries (Wenzel et al., 2011). The causative agent, hepatitis E virus (HEV), belonging to the genus Orthohepevirus, is a non-enveloped RNA virus with a single-stranded, positive-sense genome of approximately 7.2 kb (Smith et al., 2014). The genome consists of a short 5' un-translated region (UTR), three open reading frames (ORFs), and a 3' UTR containing a poly(A) tail (Meng, 2011). Four recognized major genotypes of HEV are identified: genotype 1 (Asian and African strains), genotype 2 (a Mexican strain), genotype 3 (primarily from America and Europe, and some Asian countries), and genotype 4 (mainly Asian strains) (Smith et al., 2016). Previous study revealed that HEV genotype 4 is the dominant zoonotic HEV genotype in China (Wang et al., 2016a). However, infections with HEV 3 have been found more commonly in recent years in China (Liu et al., 2012; Zhang et al., 2013). To date, only one full genome of Chinese swine genotype 3 HEV strain from Shanghai has been documented (Si et al., 2009). We report here the first full genome sequence of a genotype 3 swine HEV strain from Zhejiang, China.


结论:浙江株戊型肝炎病毒(ZhJ-PJ050-3)基因组由7223个核苷酸组成,其中3’非编码区含有23个碱基的poly(A)尾。全基因组包含ORF1、ORF2和ORF3三个主要基因,长度分别为5109、1983和342 bp。进化分析显示,ZhJ-PJ050-3为基因3型,与国内唯一报道的上海株3型毒株以及多数日本毒株共属3b亚型。与国内外戊型肝炎序列比对发现,ZhJ-PJ050-3基因组共有24个特有的点突变,且在ORF1中含有一个亮氨酸缺失。这些分子特征将为下一步的研究提供方向。


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[1]Chobe, L.P., Lole, K.S., Arankalle, V.A., 2006. Full genome sequence and analysis of Indian swine hepatitis E virus isolate of genotype 4. Vet. Microbiol., 114(3-4):240-251.

[2]Liu, P., Li, L., Wang, L., et al., 2012. Phylogenetic analysis of 626 hepatitis E virus (HEV) isolates from humans and animals in China (1986–2011) showing genotype diversity and zoonotic transmission. Infect. Genet. Evol., 12(2): 428-434.

[3]Lu, Y.H., Qian, H.Z., Qin, X., et al., 2013. Subtypes of genotype 3 hepatitis E virus in pigs. Vet. J., 197(2):509-511.

[4]Meng, X.J., 2010. Hepatitis E virus: animal reservoirs and zoonotic risk. Vet. Microbiol., 140(3-4):256-265.

[5]Meng, X.J., 2011. From barnyard to food table: the omnipresence of hepatitis E virus and risk for zoonotic infection and food safety. Virus Res., 161(1):23-30.

[6]Ning, H.Q., Niu, Z.X., Yu, R.S., et al., 2007. Identification of genotype 3 hepatitis E virus in fecal samples from a pig farm located in a Shanghai suburb. Vet. Microbiol., 121(1-2):125-130.

[7]Si, F.S., Zhu, Y.M., Dong, S.J., et al., 2009. Full genomic sequence analysis of swine genotype 3 hepatitis E virus isolated from Shanghai. Virus Res., 144(1-2):290-293.

[8]Smith, D.B., Simmonds, P., Jameel, S., et al., 2014. Consensus proposals for classification of the family Hepeviridae. J. Gen. Virol., 95(10):2223-2232.

[9]Smith, D.B., Simmonds, P., Izopet, J., et al., 2016. Proposed reference sequences for hepatitis E virus subtypes. J. Gen. Virol., 97(3):537-542.

[10]Tamura, K., Peterson, D., Peterson, N., et al., 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 28(10):2731-2739.

[11]Wang, L., Liu, L., Wei, Y.L., et al., 2016a. Clinical and virological profiling of sporadic hepatitis E virus infection in China. J. Infect., 73(3):271-279.

[12]Wang, L., Zhang, Y.L., Gong, W.Y., et al., 2016b. Hepatitis E virus in 3 types of laboratory animals, China, 2012–2015. Emerg. Infect. Dis., 22(12):2157-2159.

[13]Wenzel, J.J., Preiss, J., Schemmerer, M., et al., 2011. Detection of hepatitis E virus (HEV) from porcine livers in Southeastern Germany and high sequence homology to human HEV isolates. J. Clin. Virol., 52(1):50-54.

[14]Zhang, W., Yang, S., Shen, Q., et al., 2010a. Genotype 3 hepatitis E virus existed among swine groups in 4 geographically far regions in China. Vet. Microbiol., 140(1-2): 193-195.

[15]Zhang, W., He, Y., Wang, H., et al., 2010b. Hepatitis E virus genotype diversity in eastern China. Emerg. Infect. Dis., 16(10):1630-1632.

[16]Zhang, X.F., Li, A.Y., Shuai, J.B., et al., 2013. Validation of an internally controlled multiplex real time RT-PCR for detection and typing of HEV genotype 3 and 4. J. Virol. Methods, 193(2):432-438.

[17]Zhao, M., Li, X.J., Tang, Z.M., et al., 2015. A comprehensive study of neutralizing antigenic sites on the hepatitis E virus (HEV) capsid by constructing, clustering, and characterizing a tool box. J. Biol. Chem., 290(32):19910-19922.

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