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CLC number: S852.65

On-line Access: 2017-06-05

Received: 2016-09-22

Revision Accepted: 2016-12-16

Crosschecked: 2017-05-10

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Jiang-bing Shuai

http://orcid.org/0000-0001-6540-1962

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.6 P.549-554

http://doi.org/10.1631/jzus.B1600419


Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China


Author(s):  Jiang-bing Shuai, Lu-huan Li, Ai-yun Li, Yong-qiang He, Xiao-feng Zhang

Affiliation(s):  Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou 310016, China; more

Corresponding email(s):   zxf@zju.edu.cn

Key Words:  Swine hepatitis E virus, Full genome sequence, Phylogenetic analysis, Genotype 3


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Jiang-bing Shuai, Lu-huan Li, Ai-yun Li, Yong-qiang He, Xiao-feng Zhang. Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China[J]. Journal of Zhejiang University Science B, 2017, 18(6): 549-554.

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Abstract: 
Hepatitis E is believed to occur in both endemic and sporadic forms in developing countries, which causes a major public health problem in Asia and Africa (Meng, 2010; Wang et al., 2016a). Recent studies have documented that the disease is also endemic in many industrialized countries (Wenzel et al., 2011). The causative agent, hepatitis E virus (HEV), belonging to the genus Orthohepevirus, is a non-enveloped RNA virus with a single-stranded, positive-sense genome of approximately 7.2 kb (Smith et al., 2014). The genome consists of a short 5' un-translated region (UTR), three open reading frames (ORFs), and a 3' UTR containing a poly(A) tail (Meng, 2011). Four recognized major genotypes of HEV are identified: genotype 1 (Asian and African strains), genotype 2 (a Mexican strain), genotype 3 (primarily from America and Europe, and some Asian countries), and genotype 4 (mainly Asian strains) (Smith et al., 2016). Previous study revealed that HEV genotype 4 is the dominant zoonotic HEV genotype in China (Wang et al., 2016a). However, infections with HEV 3 have been found more commonly in recent years in China (Liu et al., 2012; Zhang et al., 2013). To date, only one full genome of Chinese swine genotype 3 HEV strain from Shanghai has been documented (Si et al., 2009). We report here the first full genome sequence of a genotype 3 swine HEV strain from Zhejiang, China.

基因3型猪戊型肝炎病毒浙江株全基因组序列分析

目的:扩增浙江地区基因3型戊型肝炎病毒全基因组序列,分析其分子特征及与国内外3型戊型肝炎病毒进行比较。
创新点:首次分析报道浙江地区基因3型猪戊型肝炎病毒全序列。
方法:利用套式PCR和末端快速扩增法对分离自浙江地区的戊型肝炎病毒进行分段扩增,经克隆测序后拼接全基因组。进一步利用生物信息学方法对基因组结构、开放阅读框及其基因型等进行分析,并与国内外戊型肝炎毒株进行比较分析。
结论:浙江株戊型肝炎病毒(ZhJ-PJ050-3)基因组由7223个核苷酸组成,其中3’非编码区含有23个碱基的poly(A)尾。全基因组包含ORF1、ORF2和ORF3三个主要基因,长度分别为5109、1983和342 bp。进化分析显示,ZhJ-PJ050-3为基因3型,与国内唯一报道的上海株3型毒株以及多数日本毒株共属3b亚型。与国内外戊型肝炎序列比对发现,ZhJ-PJ050-3基因组共有24个特有的点突变,且在ORF1中含有一个亮氨酸缺失。这些分子特征将为下一步的研究提供方向。

关键词:猪戊型肝炎病毒;全基因组序列;进化分析;基因3型

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