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Abstract: The unicellular green alga Dunaliella salina is well adapted to salt stress and contains compounds (including β-carotene and vitamins) with potential commercial value. A large transcriptome database of D. salina during the adjustment, exponential and stationary growth phases was generated using a high throughput sequencing platform. We characterized the metabolic processes in D. salina with a focus on valuable metabolites, with the aim of manipulating D. salina to achieve greater economic value in large-scale production through a bioengineering strategy. Gene expression profiles under salt stress verified using quantitative polymerase chain reaction (qPCR) implied that salt can regulate the expression of key genes. This study generated a substantial fraction of D. salina transcriptional sequences for the entire growth cycle, providing a basis for the discovery of novel genes. This first full-scale transcriptome study of D. salina establishes a foundation for further comparative genomic studies.

盐生海藻杜氏盐藻的转录组测序以及注释

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[45]List of electronic supplementary materials

[46]Data S1 Sequences of the genes identified in D. salina transcriptome

[47]Table S1 Primers of those selective genes involved in the metabolic processes in D. salina

[48]Table S2 Summary of annotation of D. salina transcriptome

[49]Table S3 Top-hit species (viridiplantae) list of D. salina BLAST-annotated uniseqs

[50]Table S4 Enzymes identified in metabolism of osmolytes (glycerol and proline), polyamines, and carotenoid through annotation of D. salina transcriptome

[51]Table S5 The best hit of the highlighted enzymes in the metabolic processes of D. salina

[52]Fig. S1 KOG (euKaryotic Ortholog Groups) functional classification of D. salina uniseqs

[53]Fig. S2 Gene ontology (GO) annotation of D. salina transcriptome

[54]Fig. S3 KEGG functional analyses of D. salina uniseqs