Full Text:   <602>

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CLC number: X511

On-line Access: 2018-06-04

Received: 2017-02-24

Revision Accepted: 2017-06-16

Crosschecked: 2018-05-14

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


De-zhao Lu


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.6 P.458-470


Proteomic analysis of human umbilical vein endothelial cells exposed to PM2.5

Author(s):  Ji Zhu, Lin-wen-si Zhu, Jin-huan Yang, Ying-ling Xu, Cui Wang, Zhuo-yu Li, Wei Mao, De-zhao Lu

Affiliation(s):  Clinical Laboratory, the Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310053, China; more

Corresponding email(s):   ludezhao@126.com

Key Words:  Fine ambient particulate matter (PM2.5), Human umbilical vein endothelial cell (HUVEC), Proteomics, Toxic mechanism

Ji Zhu, Lin-wen-si Zhu, Jin-huan Yang, Ying-ling Xu, Cui Wang, Zhuo-yu Li, Wei Mao, De-zhao Lu. Proteomic analysis of human umbilical vein endothelial cells exposed to PM2.5[J]. Journal of Zhejiang University Science B, 2018, 19(6): 458-470.

@article{title="Proteomic analysis of human umbilical vein endothelial cells exposed to PM2.5",
author="Ji Zhu, Lin-wen-si Zhu, Jin-huan Yang, Ying-ling Xu, Cui Wang, Zhuo-yu Li, Wei Mao, De-zhao Lu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Proteomic analysis of human umbilical vein endothelial cells exposed to PM2.5
%A Ji Zhu
%A Lin-wen-si Zhu
%A Jin-huan Yang
%A Ying-ling Xu
%A Cui Wang
%A Zhuo-yu Li
%A Wei Mao
%A De-zhao Lu
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 6
%P 458-470
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700103

T1 - Proteomic analysis of human umbilical vein endothelial cells exposed to PM2.5
A1 - Ji Zhu
A1 - Lin-wen-si Zhu
A1 - Jin-huan Yang
A1 - Ying-ling Xu
A1 - Cui Wang
A1 - Zhuo-yu Li
A1 - Wei Mao
A1 - De-zhao Lu
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 6
SP - 458
EP - 470
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700103

Exposure to fine ambient particulate matter (PM2.5) is known to be associated with cardiovascular disease. To uncover the molecular mechanisms involved in cardiovascular toxicity of PM2.5, we investigated alterations in the protein profile of human umbilical vein endothelial cells (HUVECs) treated with PM2.5 using two-dimensional electrophoresis in conjunction with mass spectrometry (MS). A total of 31 protein spots were selected as differentially expressed proteins and identified by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) MS. The results demonstrated that DNA damage and cell apoptosis are important factors contributing to PM2.5-mediated toxicity in HUVECs. It is further proposed that PM2.5 can inhibit superoxide dismutase (SOD) activity and increase reactive oxygen species (ROS) and malonaldehyde (MDA) production in a concentration-dependent manner. Induction of apoptosis and DNA damage through oxidative stress pathways may be one of the key toxicological events occurring in HUVECs under PM2.5 stress. These results indicated that the toxic mechanisms of PM2.5 on cardiovascular disease are related to endothelial dysfunction.


方法:培养HUVEC细胞,分为正常组(正常培养的HUVEC细胞)、PM2.5处理组(50、100 µg/ml PM2.5处理HUVEC细胞24 h).双向电泳技术建立各组细胞蛋白质组图谱,质谱技术鉴定差异表达的蛋白质,流式细胞术分析细胞凋亡情况,酶联免疫吸附法(ELISA)检测DNA损伤产物8-OHdG的水平,荧光标记技术分析DNA双链断裂的形成,并用免疫印迹法(Western blotting)检测DNA损伤相关蛋白的表达.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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