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CLC number: R49

On-line Access: 2018-04-04

Received: 2017-04-05

Revision Accepted: 2017-08-16

Crosschecked: 2018-03-08

Cited: 0

Clicked: 1447

Citations:  Bibtex RefMan EndNote GB/T7714


Li-ning Su


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.4 P.293-304


Network analysis of microRNAs, transcription factors, and target genes involved in axon regeneration

Author(s):  Li-ning Su, Xiao-qing Song, Zhan-xia Xue, Chen-qing Zheng, Hai-feng Yin, Hui-ping Wei

Affiliation(s):  Department of Basic Medicine, Hebei North University, Zhangjiakou 075029, China; more

Corresponding email(s):   whp123456@sina.com

Key Words:  Transcription factors, miRNAs, Target genes, Axon, Network analysis

Li-ning Su, Xiao-qing Song, Zhan-xia Xue, Chen-qing Zheng, Hai-feng Yin, Hui-ping Wei. Network analysis of microRNAs, transcription factors, and target genes involved in axon regeneration[J]. Journal of Zhejiang University Science B, 2018, 19(4): 293-304.

@article{title="Network analysis of microRNAs, transcription factors, and target genes involved in axon regeneration",
author="Li-ning Su, Xiao-qing Song, Zhan-xia Xue, Chen-qing Zheng, Hai-feng Yin, Hui-ping Wei",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Network analysis of microRNAs, transcription factors, and target genes involved in axon regeneration
%A Li-ning Su
%A Xiao-qing Song
%A Zhan-xia Xue
%A Chen-qing Zheng
%A Hai-feng Yin
%A Hui-ping Wei
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 4
%P 293-304
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700179

T1 - Network analysis of microRNAs, transcription factors, and target genes involved in axon regeneration
A1 - Li-ning Su
A1 - Xiao-qing Song
A1 - Zhan-xia Xue
A1 - Chen-qing Zheng
A1 - Hai-feng Yin
A1 - Hui-ping Wei
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 4
SP - 293
EP - 304
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700179

axon regeneration is crucial for recovery from neurological diseases. Numerous studies have identified several genes, microRNAs (miRNAs), and transcription factors (TFs) that influence axon regeneration. However, the regulatory networks involved have not been fully elucidated. In the present study, we analyzed a regulatory network of 51 miRNAs, 27 TFs, and 59 target genes, which is involved in axon regeneration. We identified 359 pairs of feed-forward loops (FFLs), seven important genes (Nap1l1, Arhgef12, Sema6d, Akt3, Trim2, Rab11fip2, and Rps6ka3), six important miRNAs (hsa-miR-204-5p, hsa-miR-124-3p, hsa-miR-26a-5p, hsa-miR-16-5p, hsa-miR-17-5p, and hsa-miR-15b-5p), and eight important TFs (Smada2, Fli1, Wt1, Sp6, Sp3, Smad4, Smad5, and Creb1), which appear to play an important role in axon regeneration. Functional enrichment analysis revealed that axon-associated genes are involved mainly in the regulation of cellular component organization, axonogenesis, and cell morphogenesis during neuronal differentiation. However, these findings need to be validated by further studies.


结论:通过分析,初步筛选了与神经轴突再生相关的51个miRNA、27个转录因子和59个靶标基因.进一步分析得到359对前馈环路,在此基础上推测了神经轴突再生过程中发挥重要作用的7个核心基因(Nap1l1Arhgef12Sema6dAkt3Trim2Rab11fip2Rps6ka3),6个miRNA(hsa-miR-204-5p、hsa-miR-124-3p、hsa-miR-26a-5p、hsa-miR-16-5p、hsa-miR-17-5p和hsa-miR-15b-5p)和8个转录因子(Smada2、Fli1、Wt1、Sp6、Sp3、 Smad4、Smad5和Creb1).


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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[37]List of electronic supplementary materials

[38]Table S1 Information of DEGs between Axon hESC-Neuron and Whole hESC-Neuron

[39]Table S2 Overlapping genes between DEGs and genes related to axon and genes in the same family with overlapping genes (Genes Cluster 1)

[40]Table S3 Mature miRNAs related to human axon

[41]Table S4 Target genes of miRNAs (Genes Cluster 2)

[42]Table S5 TFs interaction with 59 genes (TFs Cluster 2)

[43]Table S6 TFs interaction with 51 miRNAs (TFs Cluster 3)

[44]Table S7 Feed-forward loops in the network

[45]Table S8 Gene ontology enrichment analysis of biological process

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