CLC number: TS201.3
On-line Access: 2018-08-02
Received: 2017-07-10
Revision Accepted: 2017-12-28
Crosschecked: 2018-07-06
Cited: 0
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Lei Yuan, Faizan A. Sadiq, Tong-Jie Liu, Yang Li, Jing-Si Gu, Huan-Yi Yang, Guo-Qing He. Spoilage potential of psychrotrophic bacteria isolated from raw milk and the thermo-stability of their enzymes[J]. Journal of Zhejiang University Science B, 2018, 19(8): 630-642.
@article{title="Spoilage potential of psychrotrophic bacteria isolated from raw milk and the thermo-stability of their enzymes",
author="Lei Yuan, Faizan A. Sadiq, Tong-Jie Liu, Yang Li, Jing-Si Gu, Huan-Yi Yang, Guo-Qing He",
journal="Journal of Zhejiang University Science B",
volume="19",
number="8",
pages="630-642",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700352"
}
%0 Journal Article
%T Spoilage potential of psychrotrophic bacteria isolated from raw milk and the thermo-stability of their enzymes
%A Lei Yuan
%A Faizan A. Sadiq
%A Tong-Jie Liu
%A Yang Li
%A Jing-Si Gu
%A Huan-Yi Yang
%A Guo-Qing He
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 8
%P 630-642
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700352
TY - JOUR
T1 - Spoilage potential of psychrotrophic bacteria isolated from raw milk and the thermo-stability of their enzymes
A1 - Lei Yuan
A1 - Faizan A. Sadiq
A1 - Tong-Jie Liu
A1 - Yang Li
A1 - Jing-Si Gu
A1 - Huan-Yi Yang
A1 - Guo-Qing He
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 8
SP - 630
EP - 642
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700352
Abstract: The storage and transportation of raw milk at low temperatures promote the growth of psychrotrophic bacteria and the production of thermo-stable enzymes, which pose great threats to the quality and shelf-life of dairy products. Though many studies have been carried out on the spoilage potential of psychrotrophic bacteria and the thermo-stabilities of the enzymes they produce, further detailed studies are needed to devise an effective strategy to avoid dairy spoilage. The purpose of this study was to explore the spoilage potential of psychrotrophic bacteria from Chinese raw milk samples at both room temperature (28 °C) and refrigerated temperature (7 °C). Species of Yersinia, Pseudomonas, Serratia, and Chryseobacterium showed high proteolytic activity. The highest proteolytic activity was shown by Yersinia intermedia followed by Pseudomonas fluorescens (d). Lipolytic activity was high in isolates of Acinetobacter, and the highest in Acinetobacter guillouiae. Certain isolates showed positive β-galactosidase and phospholipase activity. Strains belonging to the same species sometimes showed markedly different phenotypic characteristics. Proteases and lipases produced by psychrotrophic bacteria retained activity after heat treatment at 70, 80, or 90 °C, and proteases appeared to be more heat-stable than lipases. For these reasons, thermo-stable spoilage enzymes produced by a high number of psychrotrophic bacterial isolates from raw milk are of major concern to the dairy industry. The results of this study provide valuable data about the spoilage potential of bacterial strains in raw milk and the thermal resistance of the enzymes they produce.
[1]Abdou AM, 2003. Purification and partial characterization of psychrotrophic Serratia marcescens lipase. J Dairy Sci, 86(1):127-132.
[2]Andersson RE, Hedlund CB, Jonsson U, 1979. Thermal inactivation of a heat-resistant lipase produced by the psychrotrophic bacterium Pseudomonas fluorescens. J Dairy Sci, 62(3):361-367.
[3]Baglinière F, Salgado RL, Salgado CA, et al., 2017. Biochemical characterization of an extracellular heat-stable protease from Serratia liquefaciens isolated from raw milk. J Food Sci, 82(4):952-959.
[4]Baruzzi F, Lagonigro R, Quintieri L, et al., 2012. Occurrence of non-lactic acid bacteria populations involved in protein hydrolysis of cold-stored high moisture Mozzarella cheese. Food Microbiol, 30(1):37-44.
[5]Baur C, Krewinkel M, Kutzli I, et al., 2015. Isolation and characterisation of a heat-resistant peptidase from Pseudomonas panacis withstanding general UHT processes. Int Dairy J, 49:46-55.
[6]Bekker A, Steyn L, Charimba G, et al., 2015. Comparison of the growth kinetics and proteolytic activities of Chryseobacterium species and Pseudomonas fluorescens. Can J Microbiol, 61(12):977-982.
[7]Bekker A, Jooste P, Steyn L, et al., 2016. Lipid breakdown and sensory analysis of milk inoculated with Chryseobacterium joostei or Pseudomonas fluorescens. Int Dairy J, 52: 101-106.
[8]Buchon L, Laurent P, Gounot AM, et al., 2000. Temperature dependence of extracellular enzymes production by psychrotrophic and psychrophilic bacteria. Biotechnol Lett, 22(19):1577-1581.
[9]Caldera L, Franzetti L, van Coillie E, et al., 2016. Identification, enzymatic spoilage characterization and proteolytic activity quantification of Pseudomonas spp. isolated from different foods. Food Microbiol, 54:142-153.
[10]Capodifoglio E, Vidal AMC, Lima JAS, et al., 2016. Lipolytic and proteolytic activity of Pseudomonas spp. isolated during milking and storage of refrigerated raw milk. J Dairy Sci, 99(7):5214-5223.
[11]Champagne CP, Laing RR, Roy D, et al., 1994. Psychrotrophs in dairy products: their effects and their control. Crit Rev Food Sci Nutr, 34(1):1-30.
[12]Chen L, Daniel RM, Coolbear T, 2003. Detection and impact of protease and lipase activities in milk and milk powders. Int Dairy J, 13(4):255-275.
[13]Chen W, Chen H, Xia Y, et al., 2009. Immobilization of recombinant thermostable β-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk. J Dairy Sci, 92(2):491-498.
[14]Cusato S, Gameiro AH, Sant'Ana AS, et al., 2014. Assessing the costs involved in the implementation of GMP and HACCP in a small dairy factory. Qual Assur Saf Crops Foods, 6(2):135-139.
[15]Decimo M, Morandi S, Silvetti T, et al., 2014. Characterization of Gram-negative psychrotrophic bacteria isolated from Italian bulk tank milk. J Food Sci, 79(10):M2081-M2090.
[16]Deeth HC, Khusniati T, Datta N, et al., 2002. Spoilage patterns of skim and whole milks. J Dairy Res, 69(2):227-241.
[17]Glück C, Rentschler E, Krewinkel M, et al., 2016. Thermostability of peptidases secreted by microorganisms associated with raw milk. Int Dairy J, 56:186-197.
[18]Gurung M, Nam HM, Tamang MD, et al., 2013. Prevalence and antimicrobial susceptibility of Acinetobacter from raw bulk tank milk in Korea. J Dairy Sci, 96(4):1997-2002.
[19]Law BA, Sharpe ME, Chapman HR, 1976. The effect of lipolytic Gram-negative psychrotrophs in stored milk on the development of rancidity in Cheddar cheese. J Dairy Res, 43(3):459-468.
[20]Lilbaek HM, Fatum TM, Ipsen R, et al., 2007. Modification of milk and whey surface properties by enzymatic hydrolysis of milk phospholipids. J Agric Food Chem, 55(8):2970-2978.
[21]Ma Y, Barbano DM, Santos M, 2003. Effect of CO2 addition to raw milk on proteolysis and lipolysis at 4°C. J Dairy Sci, 86(5):1616-1631.
[22]Machado SG, Heyndrickx M, de Block J, et al., 2016. Identification and characterization of a heat-resistant protease from Serratia liquefaciens isolated from Brazilian cold raw milk. Int J Food Microbiol, 222:65-71.
[23]Olusesan AT, Azura LK, Forghani B, et al., 2011. Purification, characterization and thermal inactivation kinetics of a non-regioselective thermostable lipase from a genotypically identified extremophilic Bacillus subtilis NS 8. New Biotechnol, 28(6):738-745.
[24]Rajmohan S, Dodd CER, Waites WM, 2002. Enzymes from isolates of Pseudomonas fluorescens involved in food spoilage. J Appl Microbiol, 93(2):205-213.
[25]Sadiq FA, Li Y, Liu TJ, et al., 2016. The heat resistance and spoilage potential of aerobic mesophilic and thermophilic spore forming bacteria isolated from Chinese milk powders. Int J Food Microbiol, 238:193-201.
[26]Santos JA, González CJ, López-Dı́az TM, et al., 1996. Extracellular protease production by dairy strains of Aeromonas hydrophila as affected by growth media and incubation temperature. Food Microbiol, 13(1):47-51.
[27]Sørhaug T, Stepaniak L, 1997. Psychrotrophs and their enzymes in milk and dairy products: quality aspects. Trends Food Sci Technol, 8(2):35-41.
[28]Stoeckel M, Lidolt M, Achberger V, et al., 2016. Growth of Pseudomonas weihenstephanensis, Pseudomonas proteolytica and Pseudomonas sp. in raw milk: impact of residual heat-stable enzyme activity on stability of UHT milk during shelf-life. Int Dairy J, 59:20-28.
[29]Teh KH, Flint S, Palmer J, et al., 2012. Proteolysis produced within biofilms of bacterial isolates from raw milk tankers. Int J Food Microbiol, 157(1):28-34.
[30]Teh KH, Lindsay D, Palmer J, et al., 2013. Lipolysis within single culture and co-culture biofilms of dairy origin. Int J Food Microbiol, 163(2-3):129-135.
[31]Titball RW, 1993. Bacterial phospholipases C. Microbiol Rev, 57(2):347-366.
[32]Vacheyrou M, Normand AC, Guyot P, et al., 2011. Cultivable microbial communities in raw cow milk and potential transfers from stables of sixteen French farms. Int J Food Microbiol, 146(3):253-262.
[33]Venter H, Osthoff G, Litthauer D, 1999. Purification and characterization of a metalloprotease from Chryseobacterium indologenes Ix9a and determination of the amino acid specificity with electrospray mass spectrometry. Protein Expr Purif, 15(3):282-295.
[34]Vithanage NR, Dissanayake M, Bolge G, et al., 2016. Biodiversity of culturable psychrotrophic microbiota in raw milk attributable to refrigeration conditions, seasonality and their spoilage potential. Int Dairy J, 57:80-90.
[35]von Neubeck M, Baur C, Krewinkel M, et al., 2015. Biodiversity of refrigerated raw milk microbiota and their enzymatic spoilage potential. Int J Food Microbiol, 211: 57-65.
[36]Wiedmann M, Weilmeier D, Dineen SS, et al., 2000. Molecular and phenotypic characterization of Pseudomonas spp. isolated from milk. Appl Environ Microbol, 66(5):2085-2095.
[37]Woods RG, Burger M, Beven CA, et al., 2001. The aprX-lipA operon of Pseudomonas fluorescens B52: a molecular analysis of metalloprotease and lipase production. Microbiology, 147(2):345-354.
[38]Yuan L, Sadiq FA, Liu TJ, et al., 2017. Psychrotrophic bacterial populations in Chinese raw dairy milk. LWT, 84:409-418.
[39]Zhang SW, Lv JP, 2014. Purification and properties of heat-stable extracellular protease from Pseudomonads fluorescens BJ-10. J Food Sci Technol, 51(6):1185-1190.
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