Full Text:   <276>

Summary:  <46>

CLC number: S311

On-line Access: 2019-01-07

Received: 2017-07-25

Revision Accepted: 2018-01-26

Crosschecked: 2018-12-05

Cited: 0

Clicked: 772

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.1 P.71-83


Effects of ZJ0273 on barley and growth recovery of herbicide-stressed seedlings through application of branched-chain amino acids

Author(s):  Ling Xu, Jian-Yao Shou, Rafaqat Ali Gill, Xiang Guo, Ullah Najeeb, Wei-Jun Zhou

Affiliation(s):  Zhejiang Key Lab of Plant Secondary Metabolism and Regulation and College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China; more

Corresponding email(s):   n.ullah@uq.edu.au, wjzhou@zju.edu.cn

Key Words:  Antioxidant enzyme activity, Barley, Branched-chain amino acid, Photosynthetic system, Reactive oxygen species, ZJ0273

Ling Xu, Jian-Yao Shou, Rafaqat Ali Gill, Xiang Guo, Ullah Najeeb, Wei-Jun Zhou. Effects of ZJ0273 on barley and growth recovery of herbicide-stressed seedlings through application of branched-chain amino acids[J]. Journal of Zhejiang University Science B, 2019, 20(8): 71-83.

@article{title="Effects of ZJ0273 on barley and growth recovery of herbicide-stressed seedlings through application of branched-chain amino acids",
author="Ling Xu, Jian-Yao Shou, Rafaqat Ali Gill, Xiang Guo, Ullah Najeeb, Wei-Jun Zhou",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of ZJ0273 on barley and growth recovery of herbicide-stressed seedlings through application of branched-chain amino acids
%A Ling Xu
%A Jian-Yao Shou
%A Rafaqat Ali Gill
%A Xiang Guo
%A Ullah Najeeb
%A Wei-Jun Zhou
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 1
%P 71-83
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer

T1 - Effects of ZJ0273 on barley and growth recovery of herbicide-stressed seedlings through application of branched-chain amino acids
A1 - Ling Xu
A1 - Jian-Yao Shou
A1 - Rafaqat Ali Gill
A1 - Xiang Guo
A1 - Ullah Najeeb
A1 - Wei-Jun Zhou
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 1
SP - 71
EP - 83
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -

In this study, we evaluated the effect of the herbicide propyl 4-(2-(4,6-dimethoxypyrimidin-2-yloxy)benzylamino) benzoate (ZJ0273) on barley growth and explored the potential to trigger growth recovery through the application of branched-chain amino acids (BCAAs). barley plants were foliar-sprayed with various concentrations of ZJ0273 (100, 500, or 1000 mg/L) at the four-leaf stage. Increasing either the herbicide concentration or measurement time after herbicide treatment significantly impaired plant morphological parameters such as plant height and biomass, and affected physiological indexes, i.e. maximal photochemical efficiency (Fv/Fm), quantum yield of photosystem II (ФPSII), net photosynthetic rate (Pn), and chlorophyll meter value (soil and plant analyzer development (SPAD)). Cellular injury of herbicide-treated plants was also evidenced by increased levels of reactive oxygen species (ROS) and antioxidative enzyme activity. Elevated levels of herbicide significantly reduced the activity of acetolactate synthase (ALS)—a key enzyme in the biosynthesis of BCAAs. In a separate experiment, growth recovery in herbicide-stressed barley plants was studied using various concentrations of BCAAs (10, 50, 100, and 200 mg/L). Increasing BCAA concentration in growth media significantly increased the biomass of herbicide-stressed barley seedlings, but had no significant effect on non-stressed plants. Further, BCAAs (100 mg/L) significantly down-regulated ROS and consequently antioxidant enzyme levels in herbicide-stressed plants. Our results showed that exogenous application of BCAAs could reverse the inhibitory effects of ZJ0273 by restoring protein biosynthesis in barley seedlings.


方法:采用水培和大田实验相结合的方法,研究了除草剂丙酯草醚对大麦生长的影响,并通过进一步测定大麦幼苗各项生理指标和支链氨基酸浓度,表明100 mg/L支链氨基酸可显著缓解丙酯草醚对大麦的生理损伤,从而恢复其生长.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Abbaspoor M, Teicher HB, Streibig JC, 2006. The effect of root-absorbed PSII inhibitors on Kautsky curve parameters in sugar beet. Weed Res, 46(3):226-235.

[2]Al-Aghabary K, Zhu ZJ, Shi QH, 2004. Influence of silicon supply on chlorophyll content, chlorophyll fluorescence, and antioxidative enzyme activities in tomato plants under salt stress. J Plant Nutr, 27(12):2101-2115.

[3]Ali E, Hussain N, Shamsi IH, et al., 2018. Role of jasmonic acid in improving tolerance of rapeseed (Brassica napus L.) to Cd toxicity. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 19(2):130-146.

[4]Anderson ME, 1985. Determination of glutathione and glutathione disulfide in biological samples. Meth Enzymol, 113: 548-570.

[5]Cakmak I, Strbac D, Marschner H, 1993. Activities of hydrogen peroxide-scavenging enzymes in germinating wheat seeds. J Exp Bot, 44(1):127-132.

[6]Chen J, Yuan J, Liu JD, et al., 2005. Mechanism of action of the novel herbicide ZJ0273. Acta Phytophy Sin, 32(1):48-52 (in Chinese).

[7]Cobb AH, Kirkwood RC, 2000. Herbicides and Their Mechanisms of Action. Sheffield Academic Press, Sheffield, England, p.73-82.

[8]Dixon DP, Cummins I, Cole DJ, et al., 1998. Glutathione-mediated detoxification systems in plants. Curr Opin Plant Biol, 1(3):258-266.

[9]Frankart C, Eullaffroy P, Vernet G, 2003. Comparative effects of four herbicides on non-photochemical fluorescence quenching in Lemna minor. Environ Exp Bot, 49(2):159-168.

[10]Guo X, Zhang F, Jin ZL, et al., 2011. Physiological effect and cytological characterization regarding susceptible response of new herbicide ZJ0273 in barley. Sci Agric Sin, 44(18):3750-3758 (in Chinese).

[11]Halliwell B, Gutteridge JMC, 1999. Free Radicals in Biology and Medicine, 3rd Ed. Oxford University Press, Oxford.

[12]Hatton PJ, Dixon D, Cole DJ, et al., 1996. Glutathione transferase activities and herbicide selectivity in maize and associated weed species. Pestic Manage Sci, 46(3):267-275.

[13]Hofgen R, Streber WR, Pohlenz HD, 1995. Antisense gene expression as a tool for evaluating molecular herbicide targets. Pestic Sci, 43(2):175-177.

[14]Irzyk GP, Fuerst EP, 1993. Purification and characterization of a glutathione S-transferase from benoxacor-treated maize (Zea mays). Plant Physiol, 102(3):803-810.

[15]Jiang MY, Zhang JH, 2001. Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol, 42(11):1265-1273.

[16]Jiang MY, Zhang JH, 2002. Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. J Exp Bot, 53(379):2401-2410.

[17]Jin ZL, Zhang F, Ahmed ZI, et al., 2010. Differential morphological and physiological responses of two oilseed Brassica species to a new herbicide ZJ0273 used in rapeseed fields. Pestic Biochem Physiol, 98(1):1-8.

[18]Law MY, Charles SA, Halliwell B, 1983. Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. The effect of hydrogen peroxide and of paraquat. Biochem J, 210(3):899-903.

[19]Leyval D, Uy D, Delaunay S, et al., 2003. Characterisation of the enzyme activities involved in the valine biosynthetic pathway in a valine-producing strain of Corynebacterium glutamicum. J Biotechnol, 104(1-3):241-252.

[20]Li Z, Han AL, Zhang YF, et al., 2009. Uptake, translocation, and distribution of root-applied [C ring-U-14C]-ZJ0273 in plants of oilseed rape and rice. J Nucl Agric Sci, 23(4):676-680 (in Chinese).

[21]Liang ZS, Ma YN, Xu T, et al., 2013. Effects of abscisic acid, gibberellin, ethylene and their interactions on production of phenolic acids in Salvia miltiorrhiza Bunge hairy roots. PLoS ONE, 8(9):e72806.

[22]Liu F, Zhang F, Jin ZL, et al., 2008. Determination of acetolactate synthase activity and protein content of oilseed rape (Brassica napus L.) leaves using visible/near-infrared spectroscopy. Anal Chim Acta, 629(1-2):56-65.

[23]Lü L, Chen J, Wu J, et al., 2004. 2-Pyrimidinyloxy-n-aryl-benzylamine derivatives, their processes and uses. US Patent 6800590.

[24]Maxwell K, Johnson GN, 2000. Chlorophyll fluorescence— a practical guide. J Exp Bot, 51(345):659-668.

[25]Momoh EJJ, Zhou W, 2001. Growth and yield responses to plant density and stage of transplanting in winter oilseed rape (Brassica napus L.). J Agron Crop Sci, 186(4):253-259.

[26]Nakano Y, Asada K, 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol, 22(5):867-880.

[27]Orcaray L, Igal M, Marino D, et al., 2010. The possible role of quinate in the mode of action of glyphosate and acetolactate synthase inhibitors. Pest Manage Sci, 66(3):262-269.

[28]Ortéga F, Bastide J, 1997. Inhibition of acetolactate synthase isozyme II from Escherichia coli by a new azido-photoaffinity sulfonylurea. Bioorg Chem, 25(4):261-274.

[29]Pandey AK, Prasad K, Singh P, et al., 1998. Comparative yield loss assessment and crop-weed association in major winter crops of mid hills of N-W Himalayas. Indian J Weed Sci, 30(1-2):54-57.

[30]Ralph PJ, Macinnis-Ng CMO, Frankart C, 2005. Fluorescence imaging application: effect of leaf age on seagrass photokinetics. Aquat Bot, 81(1):69-84.

[31]Ray TB, 1984. Site of action of chlorsulfuron: inhibition of valine and isoleucine biosynthesis in plants. Plant Physiol, 75(3):827-831.

[32]Romero-Puertas MC, McCarthy I, Gómez M, et al., 2004. Reactive oxygen species-mediated enzymatic systems involved in the oxidative action of 2,4-dichlorophenoxyacetic acid. Plant, Cell Environ, 27(9):1135-1148.

[33]Rost TL, Reynolds T, 1985. Reversal of chlorsulfuron-induced inhibition of mitotic entry by isoleucine and valine. Plant Physiol, 77(2):481-482.

[34]Royuela M, Gonzalez A, Gonzalez EM, et al., 2000. Physiological consequences of continuous, sublethal imazethapyr supply to pea plants. J Plant Physiol, 157(3):345-354.

[35]Shaner DL, Reider ML, 1986. Physiological responses of corn (Zea mays) to AC 243 997 in combination with valine, leucine, and isoleucine. Pestic Biochem Physiol, 25(2):248-257.

[36]Shaner DL, Singh BK, 1993. Phytotoxicity of acetohydroxyacid synthase inhibitors is not due to accumulation of 2-ketobutyrate and/or 2-aminobutyrate. Plant Physiol, 103(4):1221-1226.

[37]Tang QH, Chen J, Shen GH, et al., 2006. Research on application techniques for a novel herbicide 10% ZJ0273 EC on transplanted rapes. Acta Phytophy Sin, 33(3):328-332 (in Chinese).

[38]Tian T, Jin ZL, Ali B, et al., 2014. The influence of new herbicide ZJ0273 on the total- and branched-chain amino acids in oilseed rape (Brassica napus L.) leaves as revealed by near-infrared spectroscopy. Acta Physiol Plant, 36(8):2149-2156.

[39]Watson PR, Derksen DA, van Acker RC, 2006. The ability of 29 barley cultivars to compete and withstand competition. Weed Sci, 54(4):783-792.

[40]Wright TR, Bascomb NF, Sturner SF, et al., 1998. Biochemical mechanism and molecular basis for ALS-inhibiting herbicide resistance in sugarbeet (Beta vulgaris) somatic cell selections. Weed Sci, 46(1):13-23.

[41]Xia PG, Li JZ, Wang RL, et al., 2015. Comparative study on volatile oils of four Panax genus species in Southeast Asia by gas chromatography-mass spectrometry. Ind Crop Prod, 74:478-484.

[42]Xia PG, Guo HB, Zhao HG, et al., 2016. Optimal fertilizer application for Panax notoginseng and effect of soil water on root rot disease and saponin contents. J Gins Res, 40(1):38-46.

[43]Xia XJ, Huang YY, Wang L, et al., 2006. Pesticides-induced depression of photosynthesis was alleviated by 24-epibrassinolide pretreatment in Cucumis sativus L. Pestic Biochem Physiol, 86(1):42-48.

[44]Yang DF, Ma PD, Liang X, et al., 2012. Metabolic profiles and cDNA-AFLP analysis of Salvia miltiorrhiza and Salvia castanea Diel f. tomentosa Stib. PLoS ONE, 7(1):e29678.

[45]Yang YT, Peredelchuk M, Bennett GN, et al., 2000. Effect of variation of Klebsiella pneumoniae acetolactate synthase expression on metabolic flux redistribution in Escherichia coli. Biotechnol Bioeng, 69(2):150-159.

[46]Yang ZM, Ye QF, Lu L, 2008. Synthesis of herbicidal ZJ0273 labeled with tritium and carbon-14. J Labelled Compd Rad, 51(4):182-186.

[47]Zhang F, Jin ZL, Naeem MS, et al., 2009. Spatial and temporal changes in acetolactate synthase activity as affected by new herbicide ZJ0273 in rapeseed, barley and water chickweed. Pestic Biochem Physiol, 95(2):63-71.

[48]Zhang JL, Wang LL, Zheng YP, et al., 2017. Effects of Bemisia tabaci (Gennadius) infestation and squash silverleaf disorder on Cucurbita pepo L. leaf. Sci Hortic, 217:8-16.

[49]Zhang RJ, He JH, Zheng JY, et al., 2000. Weed species and their damage in wheat, barley, and rapeseed fields in Zhejiang. Acta Agric Zhejiang, 12(6):308-316 (in Chinese).

[50]Zhang WF, Zhang F, Raziuddin R, et al., 2008. Effects of 5-aminolevulinic acid on oilseed rape seedling growth under herbicide toxicity stress. J Plant Growth Regul, 27(2):159-169.

[51]Zhou QY, Liu WP, Zhang YS, et al., 2007. Action mechanisms of acetolactate synthase-inhibiting herbicides. Pestic Biochem Physiol, 89(2):89-96.

[52]Zhou W, Zhao D, Lin X, 1997. Effects of waterlogging on nitrogen accumulation and alleviation of waterlogging damage by application of nitrogen fertilizer and mixtalol in winter rape (Brassica napus L.). J Plant Growth Regul, 16(1):47-53.

[53]Zhou WJ, Leul M, 1998. Uniconazole-induced alleviation of freezing injury in relation to changes in hormonal balance, enzyme activities and lipid peroxidation in winter rape. Plant Growth Regul, 26(1):41-47.

[54]Zhu SD, Zhao SH, 1994. Analysis of amino acids by high liquid chromatography. Chin J Chromatogr, 12(1):20-24 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952276/87952783; E-mail: jzus@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE