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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.1 P.71-83

10.1631/jzus.B1700375


Effects of ZJ0273 on barley and growth recovery of herbicide-stressed seedlings through application of branched-chain amino acids


Author(s):  Ling Xu, Jian-Yao Shou, Rafaqat Ali Gill, Xiang Guo, Ullah Najeeb, Wei-Jun Zhou

Affiliation(s):  Zhejiang Key Lab of Plant Secondary Metabolism and Regulation and College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China; more

Corresponding email(s):   n.ullah@uq.edu.au, wjzhou@zju.edu.cn

Key Words:  Antioxidant enzyme activity, Barley, Branched-chain amino acid, Photosynthetic system, Reactive oxygen species, ZJ0273


Ling Xu, Jian-Yao Shou, Rafaqat Ali Gill, Xiang Guo, Ullah Najeeb, Wei-Jun Zhou. Effects of ZJ0273 on barley and growth recovery of herbicide-stressed seedlings through application of branched-chain amino acids[J]. Journal of Zhejiang University Science B, 2019, 20(8): 71-83.

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author="Ling Xu, Jian-Yao Shou, Rafaqat Ali Gill, Xiang Guo, Ullah Najeeb, Wei-Jun Zhou",
journal="Journal of Zhejiang University Science B",
volume="20",
number="1",
pages="71-83",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700375"
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%T Effects of ZJ0273 on barley and growth recovery of herbicide-stressed seedlings through application of branched-chain amino acids
%A Ling Xu
%A Jian-Yao Shou
%A Rafaqat Ali Gill
%A Xiang Guo
%A Ullah Najeeb
%A Wei-Jun Zhou
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 1
%P 71-83
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer

TY - JOUR
T1 - Effects of ZJ0273 on barley and growth recovery of herbicide-stressed seedlings through application of branched-chain amino acids
A1 - Ling Xu
A1 - Jian-Yao Shou
A1 - Rafaqat Ali Gill
A1 - Xiang Guo
A1 - Ullah Najeeb
A1 - Wei-Jun Zhou
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 1
SP - 71
EP - 83
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -


Abstract: 
In this study, we evaluated the effect of the herbicide propyl 4-(2-(4,6-dimethoxypyrimidin-2-yloxy)benzylamino) benzoate (ZJ0273) on barley growth and explored the potential to trigger growth recovery through the application of branched-chain amino acids (BCAAs). barley plants were foliar-sprayed with various concentrations of ZJ0273 (100, 500, or 1000 mg/L) at the four-leaf stage. Increasing either the herbicide concentration or measurement time after herbicide treatment significantly impaired plant morphological parameters such as plant height and biomass, and affected physiological indexes, i.e. maximal photochemical efficiency (Fv/Fm), quantum yield of photosystem II (ФPSII), net photosynthetic rate (Pn), and chlorophyll meter value (soil and plant analyzer development (SPAD)). Cellular injury of herbicide-treated plants was also evidenced by increased levels of reactive oxygen species (ROS) and antioxidative enzyme activity. Elevated levels of herbicide significantly reduced the activity of acetolactate synthase (ALS)—a key enzyme in the biosynthesis of BCAAs. In a separate experiment, growth recovery in herbicide-stressed barley plants was studied using various concentrations of BCAAs (10, 50, 100, and 200 mg/L). Increasing BCAA concentration in growth media significantly increased the biomass of herbicide-stressed barley seedlings, but had no significant effect on non-stressed plants. Further, BCAAs (100 mg/L) significantly down-regulated ROS and consequently antioxidant enzyme levels in herbicide-stressed plants. Our results showed that exogenous application of BCAAs could reverse the inhibitory effects of ZJ0273 by restoring protein biosynthesis in barley seedlings.

除草剂丙酯草醚对大麦的影响和支链氨基酸对其生长的恢复作用

目的:评价除草剂丙酯草醚对大麦生长的影响,探讨外源添加支链氨基酸对其生长的恢复作用.
创新点:从除草剂丙酯草醚作用机理出发,阐明了外源添加支链氨基酸对丙酯草醚作用下大麦各项生理和生长指标的缓解作用,为生长调节物质的进一步开发利用提供实验依据.
方法:采用水培和大田实验相结合的方法,研究了除草剂丙酯草醚对大麦生长的影响,并通过进一步测定大麦幼苗各项生理指标和支链氨基酸浓度,表明100 mg/L支链氨基酸可显著缓解丙酯草醚对大麦的生理损伤,从而恢复其生长.
结论:外源添加支链氨基酸能够缓解除草剂丙酯草醚对大麦生长的抑制作用.

关键词:抗氧化酶活性;大麦;支链氨基酸;光合系统;活性氧;丙酯草醚

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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