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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.8 P.596-609

10.1631/jzus.B1700388


An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances


Author(s):  Li-Jun Ju, Chong Zhang, Jing-Jing Liao, Yue-Peng Li, Hong-Yan Qi

Affiliation(s):  Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding email(s):   hyqiaaa@126.com

Key Words:  9-Lipoxygenase (9-LOX), Hydroperoxide lyase (HPL), Allene oxide synthase (AOS), Green leaf volatile, Jasmonic acid


Li-Jun Ju, Chong Zhang, Jing-Jing Liao, Yue-Peng Li, Hong-Yan Qi. An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances[J]. Journal of Zhejiang University Science B, 2018, 19(8): 596-609.

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author="Li-Jun Ju, Chong Zhang, Jing-Jing Liao, Yue-Peng Li, Hong-Yan Qi",
journal="Journal of Zhejiang University Science B",
volume="19",
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pages="596-609",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700388"
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%T An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances
%A Li-Jun Ju
%A Chong Zhang
%A Jing-Jing Liao
%A Yue-Peng Li
%A Hong-Yan Qi
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700388

TY - JOUR
T1 - An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances
A1 - Li-Jun Ju
A1 - Chong Zhang
A1 - Jing-Jing Liao
A1 - Yue-Peng Li
A1 - Hong-Yan Qi
J0 - Journal of Zhejiang University Science B
VL - 19
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SP - 596
EP - 609
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1700388


Abstract: 
In plants, lipoxygenases (LOXs) play a crucial role in biotic and abiotic stresses. In our previous study, five 13-LOX genes of oriental melon were regulated by abiotic stress but it is unclear whether the 9-LOX is involved in biotic and abiotic stresses. The promoter analysis revealed that CmLOX09 (type of 9-LOX) has hormone elements, signal substances, and stress elements. We analyzed the expression of CmLOX09 and its downstream genes—CmHPL and CmAOS—in the leaves of four-leaf stage seedlings of the oriental melon cultivar “Yumeiren” under wound, hormone, and signal substances. CmLOX09, CmHPL, and CmAOS were all induced by wounding. CmLOX09 was induced by auxin (indole acetic acid, IAA) and gibberellins (GA3); however, CmHPL and CmAOS showed differential responses to IAA and GA3. CmLOX09, CmHPL, and CmAOS were all induced by hydrogen peroxide (H2O2) and methyl jasmonate (MeJA), while being inhibited by abscisic acid (ABA) and salicylic acid (SA). CmLOX09, CmHPL, and CmAOS were all induced by the powdery mildew pathogen Podosphaera xanthii. The content of 2-hexynol and 2-hexenal in leaves after MeJA treatment was significantly higher than that in the control. After infection with P. xanthii, the diseased leaves of the oriental melon were divided into four levels—levels 1, 2, 3, and 4. The content of jasmonic acid (JA) in the leaves of levels 1 and 3 was significantly higher than that in the level 0 leaves. In summary, the results suggested that CmLOX09 might play a positive role in the response to MeJA through the hydroperoxide lyase (HPL) pathway to produce C6 alcohols and aldehydes, and in the response to P. xanthii through the allene oxide synthase (AOS) pathway to form JA.

薄皮甜瓜9-脂氧合酶(9-LOX)类型的CmLOX09对逆境、激素和信号类物质的响应

目的:研究CmLOX09及其下游基因CmHPLCmAOS对逆境、激素和信号类物质的响应,进一步测定茉莉酸甲酯(MeJA)处理后叶片中绿叶挥发物的含量以及接种白粉病菌后叶片中茉莉酸含量的变化,探讨脂氧合酶(LOX)响应这两种胁迫的可能途径.
创新点:通过对CmLOX09启动子中顺式作用原件的分析预测,首次研究薄皮甜瓜9-LOX类型的CmLOX09对机械损伤、激素、信号类物质以及生物胁迫的响应.
方法:利用Plant CARE软件对CmLOX09启动子响应元件进行预测分析(图S1);利用荧光定量聚合酶链反应(qRT-PCR)技术分析甜瓜在机械损伤、激素、信号类物质以及生物胁迫处理后叶片中CmLOX09CmHPLCmAOS的表达模式;利用气相色谱-质谱连用仪(GC-MS)测定叶片中绿叶挥发物的含量(图4);利用高效液相色谱-串联质谱法(HPLC-MS/MS)分析和测定叶片中茉莉酸的含量(图6).
结论:本研究结果显示:CmLOX09参与机械损伤、激素、信号类物质及白粉病菌的防御反应(图1~3,5).9-LOX类型的CmLOX09可能通过氢过氧化物裂解酶(HPL)途径产生的绿叶挥发物(GLV)来响应MeJA(图4),并通过丙二烯合酶(AOS)途径产生的茉莉酸来响应真菌胁迫(图6).综上所述,9-LOX类型的CmLOX09可能在生物和非生物胁迫反应中起重要作用.

关键词:9-脂氧合酶;氢过氧化物裂解酶;丙二烯合酶;绿叶挥发物;茉莉酸

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[55]List of electronic supplementary materials

[56]Fig. S1 Cis-regulatory elements analysis of CmLOX09 promoter

[57]Fig. S2 Level of JA in oriental melon leaves after inoculation with Podosphaera xanthii analyzed with HPLC-MS/MS

[58]Table S1 Emission of GLVs in control and MeJA treatment leaves at 6, 72, and 168 h

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