Full Text:   <399>

Summary:  <133>

CLC number: S852.4+2

On-line Access: 2018-09-30

Received: 2017-11-16

Revision Accepted: 2018-02-15

Crosschecked: 2018-09-10

Cited: 0

Clicked: 1175

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.10 P.796-806

10.1631/jzus.B1700571


A corn straw-based diet increases release of inflammatory cytokines in peripheral blood mononuclear cells of dairy cows


Author(s):  Yan-Yi Che, Xiao-Jing Xia, Bo-Ping He, Yuan-Yuan Gao, Wen-Bo Ren, Hong-Tao Liu, Jian-Fang Liu, Ting-Hao Huang, Wen-Yu Han, Lian-Cheng Lei

Affiliation(s):  College of Veterinary Medicine, Jilin University, Changchun 130062, China; more

Corresponding email(s):   leilc@jlu.edu.cn

Key Words:  Roughage, Immunity, Inflammatory cytokine, Expression profile analysis


Yan-Yi Che, Xiao-Jing Xia, Bo-Ping He, Yuan-Yuan Gao, Wen-Bo Ren, Hong-Tao Liu, Jian-Fang Liu, Ting-Hao Huang, Wen-Yu Han, Lian-Cheng Lei. A corn straw-based diet increases release of inflammatory cytokines in peripheral blood mononuclear cells of dairy cows[J]. Journal of Zhejiang University Science B, 2018, 19(10): 796-806.

@article{title="A corn straw-based diet increases release of inflammatory cytokines in peripheral blood mononuclear cells of dairy cows",
author="Yan-Yi Che, Xiao-Jing Xia, Bo-Ping He, Yuan-Yuan Gao, Wen-Bo Ren, Hong-Tao Liu, Jian-Fang Liu, Ting-Hao Huang, Wen-Yu Han, Lian-Cheng Lei",
journal="Journal of Zhejiang University Science B",
volume="19",
number="10",
pages="796-806",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700571"
}

%0 Journal Article
%T A corn straw-based diet increases release of inflammatory cytokines in peripheral blood mononuclear cells of dairy cows
%A Yan-Yi Che
%A Xiao-Jing Xia
%A Bo-Ping He
%A Yuan-Yuan Gao
%A Wen-Bo Ren
%A Hong-Tao Liu
%A Jian-Fang Liu
%A Ting-Hao Huang
%A Wen-Yu Han
%A Lian-Cheng Lei
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 10
%P 796-806
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700571

TY - JOUR
T1 - A corn straw-based diet increases release of inflammatory cytokines in peripheral blood mononuclear cells of dairy cows
A1 - Yan-Yi Che
A1 - Xiao-Jing Xia
A1 - Bo-Ping He
A1 - Yuan-Yuan Gao
A1 - Wen-Bo Ren
A1 - Hong-Tao Liu
A1 - Jian-Fang Liu
A1 - Ting-Hao Huang
A1 - Wen-Yu Han
A1 - Lian-Cheng Lei
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 10
SP - 796
EP - 806
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700571


Abstract: 
Recent studies have shown that diet can affect the body’s immunity. roughage of dairy cows consists of a variety of plant materials which make different contributions to health. This study investigated the effect of different roughages on the immunity of dairy cows. Serum, peripheral blood mononuclear cells (PBMCs), and milk samples were collected from 20 multiparous mid-lactation cows fed mixed forage (MF)- or corn straw (CS)-based diets. expression profile analysis was used to detect the differentially expressed genes (DEGs) from PBMCs. The results showed that milk protein in the MF group increased to 3.22 g/100 ml, while that of the CS group milk was 2.96 g/100 ml; by RNA sequencing, it was found that 1615 genes were differentially expressed between the CS group and the MF group among the 24 027 analyzed probes. Gene ontology (GO) and pathway analysis of DEGs suggested that these genes (especially genes coding cytokines, chemokine and its receptors) are involved in the immune response. Results were confirmed at the protein level via detecting the levels of interleukin-2 (IL-2), IL-6, IL-10, IL-12, leptin (LEP), interferon-γ (IFN-γ), transforming growth factor-β1 (TGF-β1), and tumor necrosis factor-α (TNF-α) in peripheral blood by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay analysis. Our data supported the conclusions that the protein content in milk of the MF group was higher than that of the CS group, the CS-based diets induced more release of cytokines than the MF-based diets in dairy cows’ PBMCs, and milk protein content may be affected by cytokines.

饲喂玉米秸秆粗饲料可加剧奶牛外周血单核细胞释放炎性细胞因子

目的:研究不同类型的粗饲料对奶牛外周血细胞因子的影响.
创新点:明确饲喂玉米秸秆日粮奶牛外周血单核细胞基因的表达特征,研究日粮对外周血细胞因子的影响.
方法:分别收集饲喂苜蓿混合粗饲料和玉米秸秆粗饲料的奶牛牛奶、外周血单核细胞及血清,检测牛奶营养含量,应用表达谱基因芯片分析筛选外周血单核细胞中差异表达基因,并采用荧光定量聚合酶链反应(qPCR)验证.最后采用酶联免疫吸附试验(ELISA)检测血清细胞因子的表达量.
结论:与苜蓿混合粗饲料组相比较,玉米秸秆粗饲料组的奶牛外周血单核细胞炎性细胞因子分泌水平较高,牛奶乳蛋白含量较低(P<0.05).

关键词:粗饲料;免疫;炎性细胞因子;表达谱分析

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Alemany M, 2013. Relationship between energy dense diets and white adipose tissue inflammation in metabolic syndrome. Nutr Res, 33(1):1-11.

[2]Alexandrow MG, Moses HL, 1995. Transforming growth factor β and cell cycle regulation. Cancer Res, 55(7):1452-1457.

[3]Basham B, Sathe M, Grein J, et al., 2008. In vivo identification of novel STAT5 target genes. Nucleic Acids Res, 36(11):3802-3818.

[4]Bougarn S, Cunha P, Gilbert FB, et al., 2011. Technical note: Validation of candidate reference genes for normalization of quantitative PCR in bovine mammary epithelial cells responding to inflammatory stimuli. J Dairy Sci, 94(5):2425-2430.

[5]Connor EE, Siferd S, Elsasser TH, et al., 2008. Effects of increased milking frequency on gene expression in the bovine mammary gland. BMC Genomics, 9:362.

[6]Darnell JE Jr, 1997. STATs and gene regulation. Science, 277(5332):1630-1635.

[7]Díaz-Rúa R, García-Ruiz E, Caimari A, et al., 2014. Sustained exposure to diets with an unbalanced macronutrient proportion alters key genes involved in energy homeostasis and obesity-related metabolic parameters in rats. Food Funct, 5(12):3117-3131.

[8]Gotsis E, Anagnostis P, Mariolis A, et al., 2015. Health benefits of the Mediterranean Diet: an update of research over the last 5 years. Angiology, 66(4):304-318.

[9]Huang J, Brumell JH, 2014. Bacteria-autophagy interplay: a battle for survival. Nat Rev Microbiol, 12(2):101-114.

[10]Kataoka K, 2016. The intestinal microbiota and its role in human health and disease. J Med Invest, 63(1-2):27-37.

[11]Liu JH, Xu TT, Zhu WY, et al., 2014. High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats. Br J Nutr, 112(3):416-427.

[12]Misirlioglu M, Page GP, Sagirkaya H, et al., 2006. Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos. Proc Natl Acad Sci USA, 103(50):18905-18910.

[13]Musters S, Coughlan K, McFadden T, et al., 2004. Exogenous TGF-β1 promotes stromal development in the heifer mammary gland. J Dairy Sci, 87(4):896-904.

[14]Nicholson JK, Holmes E, Kinross J, et al., 2012. Host-gut microbiota metabolic interactions. Science, 336(6086):1262-1267.

[15]Olthof ED, Roelofs HM, Versleijen MW, et al., 2013. Long-term olive oil-based parenteral nutrition sustains innate immune function in home patients without active underlying disease. Clin Nutr, 32(4):643-649.

[16]Purup S, Vestergaard M, Sejrsen K, 2000. Involvement of growth factors in the regulation of pubertal mammary growth in cattle. Adv Exp Med Biol, 480:27-43.

[17]Qin T, Wang HY, Bu DP, et al., 2013. Effect of a corn straw or mixed forage diet on endocrine, metabolism and lactation performance in periparturient cows. J Anim Vet Adv, 12(2):276-282.

[18]Rathmell JC, 2012. Metabolism and autophagy in the immune system: immunometabolism comes of age. Immunol Rev, 249(1):5-13.

[19]Schmidt JA, de Avila JM, McLean DJ, 2007. Analysis of gene expression in bovine testis tissue prior to ectopic testis tissue xenografting and during the grafting period. Biol Reprod, 76(6):1071-1080.

[20]Schwerd T, Frivolt K, Clavel T, et al., 2016. Exclusive enteral nutrition in active pediatric Crohn disease: effects on intestinal microbiota and immune regulation. J Allergy Clin Immunol, 138(2):592-596.

[21]Sethi G, Shanmugam MK, Ramachandran L, et al., 2012. Multifaceted link between cancer and inflammation. Biosci Rep, 32(1):1-15.

[22]Stecher B, 2015. The roles of inflammation, nutrient availability and the commensal microbiota in enteric pathogen infection. In: Conway T, Cohen PS (Eds.), Metabolism and Bacterial Pathogenesis. ASM Press, Washington, DC, p.297-320.

[23]Thorburn AN, Macia L, Mackay CR, 2014. Diet, metabolites, and “Western-lifestyle” inflammatory diseases. Immunity, 40(6):833-842.

[24]Wathes DC, Fenwick M, Cheng Z, et al., 2007. Influence of negative energy balance on cyclicity and fertility in the high producing dairy cow. Theriogenology, 68(S1):S232-S241.

[25]Weng XX, Bu DP, Li FD, et al., 2012. Responses in milk yield, milk composition and rumen fermentation in lactating cows receiving a corn straw or mixed forage diet. J Anim Vet Adv, 11:4678-4683.

[26]Wu WJ, Lee CF, Hsin CH, et al., 2008. TGF-β inhibits prolactin-induced expression of β-casein by a Smad3-dependent mechanism. J Cell Biochem, 104(5):1647-1659.

[27]Xia XJ, Che YY, Gao YY, et al., 2016. Arginine supplementation recovered the IFN-γ-mediated decrease in milk protein and fat synthesis by inhibiting the GCN2/eIF2α pathway, which induces autophagy in primary bovine mammary epithelial cells. Mol Cells, 39(5):410-417.

[28]Zhou J, Dong GZ, Ao CJ, et al., 2014. Feeding a high-concentrate corn straw diet increased the release of endotoxin in the rumen and pro-inflammatory cytokines in the mammary gland of dairy cows. BMC Vet Res, 10:172.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE