CLC number: S41-30
On-line Access: 2019-04-01
Received: 2018-11-06
Revision Accepted: 2019-01-29
Crosschecked: 2019-03-02
Cited: 0
Clicked: 4268
De-Qing Huang, Rui Chen, Ya-Qin Wang, Jian Hong, Xue-Ping Zhou, Jian-Xiang Wu. Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus[J]. Journal of Zhejiang University Science B, 2019, 20(4): 343-354.
@article{title="Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus",
author="De-Qing Huang, Rui Chen, Ya-Qin Wang, Jian Hong, Xue-Ping Zhou, Jian-Xiang Wu",
journal="Journal of Zhejiang University Science B",
volume="20",
number="4",
pages="343-354",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800563"
}
%0 Journal Article
%T Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus
%A De-Qing Huang
%A Rui Chen
%A Ya-Qin Wang
%A Jian Hong
%A Xue-Ping Zhou
%A Jian-Xiang Wu
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 4
%P 343-354
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800563
TY - JOUR
T1 - Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus
A1 - De-Qing Huang
A1 - Rui Chen
A1 - Ya-Qin Wang
A1 - Jian Hong
A1 - Xue-Ping Zhou
A1 - Jian-Xiang Wu
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 4
SP - 343
EP - 354
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800563
Abstract: rice stripe virus%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>rice stripe virus (RSV) causes dramatic losses in rice production worldwide. In this study, two monoclonal antibodies (MAbs) 16E6 and 11C1 against RSV and a colloidal gold-based immunochromatographic strip were developed for specific, sensitive, and rapid detection of RSV in rice plant and planthopper samples. The MAb 16E6 was conjugated with colloidal gold and the MAb 11C1 was coated on the test line of the nitrocellulose membrane of the test strip. The specificity of the test strip was confirmed by a positive reaction to RSV-infected rice plants and small brown planthopper (SBPH), and negative reactions to five other rice viruses, healthy rice plants, four other vectors of five rice viruses, and non-viruliferous SBPH. Sensitivity analyses showed that the test strip could detect the virus in RSV-infected rice plant tissue crude extracts diluted to 1:20 480 (w/v, g/mL), and in individual viruliferous SBPH homogenate diluted to 1:2560 (individual SPBH/μL). The validity of the developed strip was further confirmed by tests using field-collected rice and SBPH samples. This newly developed test strip is a low-cost, fast, and easy-to-use tool for on-site detection of RSV infection during field epidemiological studies and paddy field surveys, and thus can benefit decision-making for RSV management in the field.
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