CLC number: S852.65+9.2
On-line Access: 2020-07-07
Received: 2019-11-23
Revision Accepted: 2020-02-16
Crosschecked: 2020-06-05
Cited: 0
Clicked: 3264
Shi-chao Geng, Xiao-liang Li, Wei-huan Fang. Porcine circovirus 3 capsid protein induces autophagy in HEK293T cells by inhibiting phosphorylation of the mammalian target of rapamycin[J]. Journal of Zhejiang University Science B, 2020, 21(7): 560-570.
@article{title="Porcine circovirus 3 capsid protein induces autophagy in HEK293T cells by inhibiting phosphorylation of the mammalian target of rapamycin",
author="Shi-chao Geng, Xiao-liang Li, Wei-huan Fang",
journal="Journal of Zhejiang University Science B",
volume="21",
number="7",
pages="560-570",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900657"
}
%0 Journal Article
%T Porcine circovirus 3 capsid protein induces autophagy in HEK293T cells by inhibiting phosphorylation of the mammalian target of rapamycin
%A Shi-chao Geng
%A Xiao-liang Li
%A Wei-huan Fang
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 7
%P 560-570
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900657
TY - JOUR
T1 - Porcine circovirus 3 capsid protein induces autophagy in HEK293T cells by inhibiting phosphorylation of the mammalian target of rapamycin
A1 - Shi-chao Geng
A1 - Xiao-liang Li
A1 - Wei-huan Fang
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 7
SP - 560
EP - 570
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900657
Abstract: porcine circovirus 3 (PCV3) has been detected in major pig-producing countries around the world since its first report in the US in 2016. Most current studies have focused on epidemiological investigations and detection methods of PCV3 because of lack of live virus strains for research on its pathogenesis in porcine cells or even in pigs. We constructed a recombinant plasmid pCMV-Cap carrying the PCV3 orf2 gene to investigate the effects of capsid (Cap) protein expression on autophagic response in human embryonic kidney cell line 293T (HEK293T). We demonstrate that PCV3 Cap protein induced complete autophagy shown as formation of autophagosomes and autophagosome-like vesicles as well as LC3-II conversion from LC3-I via inhibiting phosphorylation of the mammalian target of rapamycin (mTOR) in HEK293T cells. The ubiquitin–proteasome pathway is also involved in the autophagy process. These findings provide insight for further exploration of PCV3 pathogenetic mechanisms in porcine cells.
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