Full Text:   <187>

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CLC number: S91

On-line Access: 2020-10-12

Received: 2020-03-17

Revision Accepted: 2020-07-22

Crosschecked: 2020-09-07

Cited: 0

Clicked: 305

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Mohamad Nor Azra

https://orcid.org/0000-0001-9333-9270

Mhd Ikhwanuddin

https://orcid.org/0000-0002-6430-0876

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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.10 P.823-834

http://doi.org/10.1631/jzus.B2000126


Amino acid compounds released by the giant freshwater prawn Macrobrachium rosenbergii during ecdysis: a factor attracting cannibalistic behaviour?


Author(s):  Abu Seman Juneta-Nor, Noordiyana Mat Noordin, Mohamad Nor Azra, Hong-yu Ma, Norainy Mohd Husin, Mhd Ikhwanuddin

Affiliation(s):  Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu 21030, Malaysia; more

Corresponding email(s):   azramn@umt.edu.my, ikhwanuddin@umt.edu.my

Key Words:  Amino acid compound, Cannibalism, Chemical cue, Giant freshwater prawn, Moulting


Abu Seman Juneta-Nor, Noordiyana Mat Noordin, Mohamad Nor Azra, Hong-yu Ma, Norainy Mohd Husin, Mhd Ikhwanuddin. Amino acid compounds released by the giant freshwater prawn Macrobrachium rosenbergii during ecdysis: a factor attracting cannibalistic behaviour?[J]. Journal of Zhejiang University Science B, 2020, 21(10): 823-834.

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author="Abu Seman Juneta-Nor, Noordiyana Mat Noordin, Mohamad Nor Azra, Hong-yu Ma, Norainy Mohd Husin, Mhd Ikhwanuddin",
journal="Journal of Zhejiang University Science B",
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number="10",
pages="823-834",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2000126"
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%T Amino acid compounds released by the giant freshwater prawn Macrobrachium rosenbergii during ecdysis: a factor attracting cannibalistic behaviour?
%A Abu Seman Juneta-Nor
%A Noordiyana Mat Noordin
%A Mohamad Nor Azra
%A Hong-yu Ma
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A1 - Abu Seman Juneta-Nor
A1 - Noordiyana Mat Noordin
A1 - Mohamad Nor Azra
A1 - Hong-yu Ma
A1 - Norainy Mohd Husin
A1 - Mhd Ikhwanuddin
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B2000126


Abstract: 
Ecdysis is a common phenomenon that happens throughout the life phase of the giant freshwater prawn Macrobrachium rosenbergii. It is vital to better understand the correlation between cannibalism and biochemical compound that exists during the moulting process. The objective of the present study was to determine the amino acid profile released by M. rosenbergii during the ecdysis process that promotes cannibalism. To accomplish this, changes in amino acid levels (total amino acid (TAA) and free amino acid (FAA)) of tissue muscle, exoskeleton, and sample water of culture medium from the moulting (E-stage) and non-moulting (C-stage) prawns were analysed using high-performance liquid chromatography (HPLC). Comparison study revealed that among the TAA compounds, proline and sarcosine of tissues from moulting prawn were found at the highest levels. The level of FAA from water that contains moulting prawns (E-stage) was dominated by tryptophan and proline. Significant values obtained in the present study suggested that these amino acid compounds act as a chemical cue to promote cannibalism in M. rosenbergii during ecdysis. The knowledge of compositions and compounds that were released during the moulting process should be helpful for better understanding of the mechanism and chemical cues that play roles on triggering cannibalism, and also for future dietary manipulation to improve feeding efficiencies and feeding management, which indirectly impacts productivity and profitability.

罗氏沼虾蜕皮过程中释放的氨基酸组分是引发同类残杀的因素?

目的:确定罗氏沼虾在蜕皮过程(该过程引起同类残杀)中释放的氨基酸组分,并明确其中差异的氨基酸成分.
创新点:了解罗氏沼虾蜕皮过程中生化组分的变化对于理解同类残杀的重要意义.目前,还没有关于同类残杀和蜕皮过程与生化组分之间关系的研究报道.本研究以此为切入点,采用统计学方法探究二者之间的关联,并鉴定其中差异的氨基酸成分.
方法:在人工饲养条件下,测定了蜕皮阶段和非蜕皮阶段的罗氏沼虾肌肉、外骨骼和养殖水体中的总氨基酸及自由氨基酸组分,采用双因素方差分析法比较分析了两个阶段氨基酸组分的差异,同时鉴定了差异的氨基酸成分.
结论:在蜕皮阶段,组织中的总氨基酸以脯氨酸和肌氨酸的含量最高,而水体中的自由氨基酸含量以色氨酸和脯氨酸为主.研究结果表明,这些氨基酸组分在罗氏沼虾蜕皮过程中释放了促进同类残杀的化学信号.

关键词:氨基酸组分;同类残杀;化学信号;罗氏沼虾;蜕皮

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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