Full Text:   <118>

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On-line Access: 2020-11-05

Received: 2020-07-06

Revision Accepted: 2020-09-02

Crosschecked: 2020-10-10

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Citations:  Bibtex RefMan EndNote GB/T7714


Dan Yu


Jin Wang


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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.11 P.871-884


Effects of nanofibers on mesenchymal stem cells: environmental factors affecting cell adhesion and osteogenic differentiation and their mechanisms

Author(s):  Dan Yu, Jin Wang, Ke-jia Qian, Jing Yu, Hui-yong Zhu

Affiliation(s):  Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; more

Corresponding email(s):   zhuhuiyong@zju.edu.cn

Key Words:  Nanofiber, Stem cell, Mimicking natural tissue, Morphology, Signaling pathway

Dan Yu, Jin Wang, Ke-jia Qian, Jing Yu, Hui-yong Zhu. Effects of nanofibers on mesenchymal stem cells: environmental factors affecting cell adhesion and osteogenic differentiation and their mechanisms[J]. Journal of Zhejiang University Science B, 2020, 21(11): 871-884.

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nanofibers can mimic natural tissue structure by creating a more suitable environment for cells to grow, prompting a wide application of nanofiber materials. In this review, we include relevant studies and characterize the effect of nanofibers on mesenchymal stem cells, as well as factors that affect cell adhesion and osteogenic differentiation. We hypothesize that the process of bone regeneration in vitro is similar to bone formation and healing in vivo, and the closer nanofibers or nanofibrous scaffolds are to natural bone tissue, the better the bone regeneration process will be. In general, cells cultured on nanofibers have a similar gene expression pattern and osteogenic behavior as cells induced by osteogenic supplements in vitro. Genes involved in cell adhesion (focal adhesion kinase (FAK)), cytoskeletal organization, and osteogenic pathways (transforming growth factor-β (TGF-β)/bone morphogenic protein (BMP), mitogen-activated protein kinase (MAPK), and Wnt) are upregulated successively. Cell adhesion and osteogenesis may be influenced by several factors. nanofibers possess certain physical properties including favorable hydrophilicity, porosity, and swelling properties that promote cell adhesion and growth. Moreover, nanofiber stiffness plays a vital role in cell fate, as cell recruitment for osteogenesis tends to be better on stiffer scaffolds, with associated signaling pathways of integrin and Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ). Also, hierarchically aligned nanofibers, as well as their combination with functional additives (growth factors, HA particles, etc.), contribute to osteogenesis and bone regeneration. In summary, previous studies have indicated that upon sensing the stiffness of the nanofibrous environment as well as its other characteristics, stem cells change their shape and tension accordingly, regulating downstream pathways followed by adhesion to nanofibers to contribute to osteogenesis. However, additional experiments are needed to identify major signaling pathways in the bone regeneration process, and also to fully investigate its supportive role in fabricating or designing the optimum tissue-mimicking nanofibrous scaffolds.


概要:纳米纤维可以建造适合细胞生长的环境,这种仿生性能促进了纳米纤维材料的广泛应用.在这篇综述中,我们检索了相关研究,并归纳总结了纳米纤维对间充质干细胞的影响,以及影响细胞粘附和成骨分化的因素.我们假设:体外的骨再生过程与体内骨形成和愈合的过程类似;纳米纤维或其支架材料与天然骨组织越接近,骨再生过程就越好.通常,在纳米纤维上培养的细胞具有与体外成骨诱导下的细胞相似的基因表达模式 和成骨分化.在此过程中,诸多基因通路表达相继上调(例如,与细胞粘附有关的基因FAK(黏着斑激酶)、与细胞骨架变化和成骨分化有关的基因通路转化生长因子-β(TGF-β)/骨形态发生蛋白(BMP)、促分裂原活化蛋白激酶(MAPK)和Wnt等).细胞粘附和成骨分化可能受到多种因素的影响.纳米纤维的某些物理性质能够促进细胞粘附和生长,包括合适的亲水性、孔隙率和溶胀性.此外,纳米纤维的硬度在细胞命运中起着至关重要的作用,在较坚硬的支架上,成骨细胞的募集往往更好,其中整合素和YAP/TAZ信号通路与之密切相关.同时,纳米纤维的分层排列结构以及它们与功能性添加剂(生长因子、羟基磷灰石颗粒等)的组合也有助于成骨和骨再生.总而言之,在检测到纳米纤维环境的硬度及其他特征后,干细胞会相应地改变其形状和张力,调节下游路径,接着粘附至纳米纤维并开始成骨分化.然而,成骨分化过程中的主要信号通路还需要更多实验证实,从而为设计及制作最理想的仿生纳米纤维支架提供理论支持.

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[1]Abedin E, Lari R, Mahdavi Shahri N, et al., 2018. Development of a demineralized and decellularized human epiphyseal bone scaffold for tissue engineering: a histological study. Tissue Cell, 55:46-52.

[2]Allori AC, Sailon AM, Warren SM, 2008. Biological basis of bone formation, remodeling, and repair—part II: extracellular matrix. Tissue Eng Part B: Rev, 14(3):275-283.

[3]Andalib MN, Lee JS, Ha L, et al., 2013. The role of RhoA kinase (ROCK) in cell alignment on nanofibers. Acta Biomater, 9(8):7737-7745.

[4]Andalib MN, Lee JS, Ha L, et al., 2016. Focal adhesion kinase regulation in stem cell alignment and spreading on nanofibers. Biochem Biophys Res Commun, 473(4):920-925.

[5]Arslan E, Hatip Koc M, Uysal O, et al., 2017. Supramolecular peptide nanofiber morphology affects mechanotransduction of stem cells. Biomacromolecules, 18(10):3114-3130.

[6]Asencio IO, Mittar S, Sherborne C, et al., 2018. A methodology for the production of microfabricated electrospun membranes for the creation of new skin regeneration models. J Tissue Eng, 9:1-8.

[7]Baker BA, Pine PS, Chatterjee K, et al., 2014. Ontology analysis of global gene expression differences of human bone marrow stromal cells cultured on 3D scaffolds or 2D films. Biomaterials, 35(25):6716-6726.

[8]Barros RC, Gelens E, Bulten E, et al., 2017. Self-assembled nanofiber coatings for controlling cell responses. J Biomed Mater Res A, 105(8):2252-2265.

[9]Bhardwaj N, Kundu SC, 2010. Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv, 28(3):325-347.

[10]Brusatin G, Panciera T, Gandin A, et al., 2018. Biomaterials and engineered microenvironments to control YAP/TAZ-dependent cell behaviour. Nat Mater, 17(12):1063-1075.

[11]Canha-Gouveia A, Rita Costa-Pinto A, Martins AM, et al., 2015. Hierarchical scaffolds enhance osteogenic differentiation of human Wharton’s jelly derived stem cells. Biofabrication, 7(3):035009.

[12]Carbone EJ, Jiang T, Nelson C, et al., 2014. Small molecule delivery through nanofibrous scaffolds for musculoskeletal regenerative engineering. Nanomedicine: NBM, 10(8):1691-1699.

[13]Ceylan H, Kocabey S, Gulsuner HU, et al., 2014. Bone-like mineral nucleating peptide nanofibers induce differentiation of human mesenchymal stem cells into mature osteoblasts. Biomacromolecules, 15(7):2407-2418.

[14]Chaires-Rosas CP, Ambriz X, Montesinos JJ, et al., 2019. Differential adhesion and fibrinolytic activity of mesenchymal stem cells from human bone marrow, placenta, and Wharton’s jelly cultured in a fibrin hydrogel. J Tissue Eng, 10:1-17.

[15]Chang B, Ma C, Liu XH, 2018. Nanofibers regulate single bone marrow stem cell osteogenesis via FAK/RhoA/YAP1 pathway. ACS Appl Mater Interfaces, 10(39):33022-33031.

[16]Chaudhuri O, Gu L, Klumpers D, et al., 2016. Hydrogels with tunable stress relaxation regulate stem cell fate and activity. Nat Mater, 15(3):326-334.

[17]Chen GQ, Deng CX, Li YP, 2012. TGF-β and BMP signaling in osteoblast differentiation and bone formation. Int J Biol Sci, 8(2):272-288.

[18]Chen HL, Malheiro ADBF, van Blitterswijk C, et al., 2017. Direct writing electrospinning of scaffolds with multidimensional fiber architecture for hierarchical tissue engineering. ACS Appl Mater Interfaces, 9(44):38187-38200.

[19]Chen XN, Fu XL, Shi JG, et al., 2013. Regulation of the osteogenesis of pre-osteoblasts by spatial arrangement of electrospun nanofibers in two- and three-dimensional environments. Nanomedicine: NBM, 9(8):1283-1292.

[20]Cheng Z, Ye Z, Natan A, et al., 2019. Bone-inspired mineralization with highly aligned cellulose nanofibers as template. ACS Appl Mater Interfaces, 11(45):42486-42495.

[21]Cui CB, Cooper LF, Yang XL, et al., 2003. Transcriptional coactivation of bone-specific transcription factor Cbfa1 by TAZ. Mol Cell Biol, 23(3):1004-1013.

[22]Dalby MJ, Gadegaard N, Oreffo ROC, 2014. Harnessing nanotopography and integrin–matrix interactions to influence stem cell fate. Nat Mater, 13(6):558-569.

[23]Das A, Fischer RS, Pan DJ, et al., 2016. YAP nuclear localization in the absence of cell–cell contact is mediated by a filamentous actin-dependent, myosin II- and phosphor-YAP-independent pathway during extracellular matrix mechanosensing. J Biol Chem, 291(12):6096-6110.

[24]di Cio S, Gautrot JE, 2016. Cell sensing of physical properties at the nanoscale: mechanisms and control of cell adhesion and phenotype. Acta Biomater, 30:26-48.

[25]Discher DE, Janmey P, Wang YL, 2005. Tissue cells feel and respond to the stiffness of their substrate. Science, 310(5751):1139-1143.

[26]Doosti-Telgerd M, Mahdavi FS, Moradikhah F, et al., 2020. Nanofibrous scaffolds containing hydroxyapatite and microfluidic-prepared polyamidoamin/BMP-2 plasmid dendriplexes for bone tissue engineering applications. Int J Nanomed, 15:2633-2646.

[27]Duan B, Shou KQ, Su XJ, et al., 2017. Hierarchical microspheres constructed from chitin nanofibers penetrated hydroxyapatite crystals for bone regeneration. Biomacromolecules, 18(7):2080-2089.

[28]Dufort CC, Paszek MJ, Weaver VM, 2011. Balancing forces: architectural control of mechanotransduction. Nat Rev Mol Cell Biol, 12(5):308-319.

[29]Dupont S, Morsut L, Aragona M, et al., 2011. Role of YAP/TAZ in mechanotransduction. Nature, 474(7350):179-183.

[30]Engler AJ, Sen S, Sweeney HL, et al., 2006. Matrix elasticity directs stem cell lineage specification. Cell, 126(4):677-689.

[31]Fratzl P, Weinkamer R, 2007. Hierarchical structure and repair of bone: deformation, remodelling, healing. In: van der Zwaag S (Ed.), Self Healing Materials. Springer Series in Materials Science, Vol. 100. Springer, Dordrecht, p.323-335.

[32]Gao X, Zhang XH, Song JL, et al., 2015. Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering. Int J Nanomed, 10(1):7109-7128.

[33]Guilak F, Cohen DM, Estes BT, et al., 2009. Control of stem cell fate by physical interactions with the extracellular matrix. Cell Stem Cell, 5(1):17-26.

[34]Gupte MJ, Swanson WB, Hu J, et al., 2018. Pore size directs bone marrow stromal cell fate and tissue regeneration in nanofibrous macroporous scaffolds by mediating vascularization. Acta Biomater, 82:1-11.

[35]Higgins AM, Banik BL, Brown JL, 2015. Geometry sensing through POR1 regulates Rac1 activity controlling early osteoblast differentiation in response to nanofiber diameter. Integr Biol, 7(2):229-236.

[36]Hong JH, Hwang ES, McManus MT, et al., 2005. TAZ, a transcriptional modulator of mesenchymal stem cell differentiation. Science, 309(5737):1074-1078.

[37]Hosseini FS, Soleimanifar F, Khojasteh A, et al., 2019. Promoting osteogenic differentiation of human-induced pluripotent stem cells by releasing WNT/β-catenin signaling activator from the nanofibers. J Cell Biochem, 120(4):6339-6346.

[38]Huebsch N, Arany PR, Mao AS, et al., 2010. Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate. Nat Mater, 9(6):518-526.

[39]Hwang CW, Johnston PV, Gerstenblith G, et al., 2015. Stem cell impregnated nanofiber stent sleeve for on-stent production and intravascular delivery of paracrine factors. Biomaterials, 52:318-326.

[40]Izadpanahi M, Seyedjafari E, Arefian E, et al., 2018. Nanotopographical cues of electrospun PLLA efficiently modulate non-coding RNA network to osteogenic differentiation of mesenchymal stem cells during BMP signaling pathway. Mater Sci Eng C, 93:686-703.

[41]Jahanmard F, Eslaminejad MB, Amani-Tehran M, et al., 2020. Incorporation of F-MWCNTs into electrospun nanofibers regulates osteogenesis through stiffness and nanotopography. Mater Sci Eng C, 106:110163.

[42]Kegelman CD, Mason DE, Dawahare JH, et al., 2018. Skeletal cell YAP and TAZ combinatorially promote bone development. FASEB J, 32(5):2706-2721.

[43]Kennedy KM, Bhaw-Luximon A, Jhurry D, 2017. Cell–matrix mechanical interaction in electrospun polymeric scaffolds for tissue engineering: implications for scaffold design and performance. Acta Biomater, 50:41-55.

[44]Khorshidi S, Karkhaneh A, 2018. Hydrogel/fiber conductive scaffold for bone tissue engineering. J Biomed Mater Res Part A, 106(3):718-724.

[45]Kim JJ, El-Fiqi A, Kim HW, 2017. Synergetic cues of bioactive nanoparticles and nanofibrous structure in bone scaffolds to stimulate osteogenesis and angiogenesis. ACS Appl Mater Interfaces, 9(3):2059-2073.

[46]Kim MS, Shin YN, Cho MH, et al., 2007. Adhesion behavior of human bone marrow stromal cells on differentially wettable polymer surfaces. Tissue Eng, 13(8):2095-2103.

[47]Kuang R, Zhang ZP, Jin XB, et al., 2016. Nanofibrous spongy microspheres for the delivery of hypoxia-primed human dental pulp stem cells to regenerate vascularized dental pulp. Acta Biomater, 33:225-234.

[48]Lee JH, Kim HW, 2018. Emerging properties of hydrogels in tissue engineering. J Tissue Eng, 9:2041731418768285.

[49]Lee JH, Lee YJ, Cho HJ, et al., 2014. Guidance of in vitro migration of human mesenchymal stem cells and in vivo guided bone regeneration using aligned electrospun fibers. Tissue Eng Part A, 20(15-16):2031-2042.

[50]Li HX, Wu T, Xue JJ, et al., 2020. Transforming nanofiber mats into hierarchical scaffolds with graded changes in porosity and/or nanofiber alignment. Macromol Rapid Commun, 41(3):1900579.

[51]Liu HH, Peng HJ, Wu Y, et al., 2013. The promotion of bone regeneration by nanofibrous hydroxyapatite/chitosan scaffolds by effects on integrin-BMP/Smad signaling pathway in BMSCs. Biomaterials, 34(18):4404-4417.

[52]Liu L, Kamei KI, Yoshioka M, et al., 2017. Nano-on-micro fibrous extracellular matrices for scalable expansion of human ES/iPS cells. Biomaterials, 124:47-54.

[53]Liu M, Zeng X, Ma C, et al., 2017. Injectable hydrogels for cartilage and bone tissue engineering. Bone Res, 5:17014.

[54]Liu WT, Wei Y, Zhang XH, et al., 2013. Lower extent but similar rhythm of osteogenic behavior in hBMSCs cultured on nanofibrous scaffolds versus induced with osteogenic supplement. ACS Nano, 7(8):6928-6938.

[55]Liu XY, Shen H, Song SJ, et al., 2017. Accelerated biomineralization of graphene oxide-incorporated cellulose acetate nanofibrous scaffolds for mesenchymal stem cell osteogenesis. Colloids Surf B Biointerfaces, 159:251-258.

[56]Liu Y, Luo D, Wang T, 2016. Hierarchical structures of bone and bioinspired bone tissue engineering. Small, 12(34):4611-4632.

[57]Lü LX, Wang YY, Mao X, et al., 2012. The effects of PHBV electrospun fibers with different diameters and orientations on growth behavior of bone-marrow-derived mesenchymal stem cells. Biomed Mater, 7:015002.

[58]Luo Y, Shen H, Fang YX, et al., 2015. Enhanced proliferation and osteogenic differentiation of mesenchymal stem cells on graphene oxide-incorporated electrospun poly(lactic-co-glycolic acid) nanofibrous mats. ACS Appl Mater Interfaces, 7(11):6331-6339.

[59]Lv HW, Wang HP, Zhang ZJ, et al., 2017. Biomaterial stiffness determines stem cell fate. Life Sci, 178:42-48.

[60]Mahmoudi N, Simchi A, 2017. On the biological performance of graphene oxide-modified chitosan/polyvinyl pyrrolidone nanocomposite membranes: in vitro and in vivo effects of graphene oxide. Mater Sci Eng C, 70:121-131.

[61]Mao AS, Shin JW, Mooney DJ, 2016. Effects of substrate stiffness and cell–cell contact on mesenchymal stem cell differentiation. Biomaterials, 98:184-191.

[62]Marrella A, Tedeschi G, Giannoni P, et al., 2018. “Green-reduced” graphene oxide induces in vitro an enhanced biomimetic mineralization of polycaprolactone electrospun meshes. Mater Sci Eng C, 93:1044-1053.

[63]McBeath R, Pirone DM, Nelson CM, et al., 2004. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell, 6(4):483-495.

[64]Mobasseri R, Tian LL, Soleimani M, et al., 2018. Peptide modified nanofibrous scaffold promotes human mesenchymal stem cell proliferation and long-term passaging. Mater Sci Eng C, 84:80-89.

[65]Moradi SL, Golchin A, Hajishafieeha Z, et al., 2018. Bone tissue engineering: adult stem cells in combination with electrospun nanofibrous scaffolds. J Cell Physiol, 233(10):6509-6522.

[66]Motamedian SR, Hosseinpour S, Ahsaie MG, et al., 2015. Smart scaffolds in bone tissue engineering: a systematic review of literature. World J Stem Cells, 7(3):657-668.

[67]Nam J, Johnson J, Lannutti JJ, et al., 2011. Modulation of embryonic mesenchymal progenitor cell differentiation via control over pure mechanical modulus in electrospun nanofibers. Acta Biomater, 7(4):1516-1524.

[68]Namdari M, Negahdari B, Eatemadi A, 2017. Paediatric nanofibrous bioprosthetic heart valve. IET Nanobiotechnol, 11(5):493-500.

[69]Nasajpour A, Mandla S, Shree S, et al., 2017. Nanostructured fibrous membranes with rose spike-like architecture. Nano Lett, 17(10):6235-6240.

[70]Noori A, Ashrafi SJ, Vaez-Ghaemi R, et al., 2017. A review of fibrin and fibrin composites for bone tissue engineering. Int J Nanomed, 12:4937-4961.

[71]Olivares-Navarrete R, Lee EM, Smith K, et al., 2017. Substrate stiffness controls osteoblastic and chondrocytic differentiation of mesenchymal stem cells without exogenous stimuli. PLoS ONE, 12(1):e0170312.

[72]Ortega Z, Alemán ME, Donate R, 2018. Nanofibers and microfibers for osteochondral tissue engineering. In: Oliveira JM, Pina S, Reis RL, et al. (Eds.), Osteochondral Tissue Engineering. Advances in Experimental Medicine and Biology, Vol. 1058. Springer, Cham, p.97-123.

[73]Ozdemir T, Xu LC, Siedlecki C, et al., 2013. Substrate curvature sensing through myosin IIA upregulates early osteogenesis. Integr Biol, 5(11):1407-1416.

[74]Pan HH, Xie YT, Zhang ZQ, et al., 2017. YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure. Colloids Surf B Biointerfaces, 152:344-353.

[75]Pan JX, Xiong L, Zhao K, et al., 2018. YAP promotes osteogenesis and suppresses adipogenic differentiation by regulating β-catenin signaling. Bone Res, 6:18.

[76]Panciera T, Azzolin L, Cordenonsi M, et al., 2017. Mechanobiology of YAP and TAZ in physiology and disease. Nat Rev Mol Cell Biol, 18(12):758-770.

[77]Pandey S, Rathore K, Johnson J, et al., 2018. Aligned nanofiber material supports cell growth and increases osteogenesis in canine adipose-derived mesenchymal stem cells in vitro. J Biomed Mater Res Part A, 106(7):1780-1788.

[78]Perikamana SKM, Lee J, Ahmad T, et al., 2015. Effects of immobilized BMP-2 and nanofiber morphology on in vitro osteogenic differentiation of hMSCs and in vivo collagen assembly of regenerated bone. ACS Appl Mater Interfaces, 7(16):8798-8808.

[79]Piccolo S, Dupont S, Cordenonsi M, 2014. The biology of YAP/TAZ: hippo signaling and beyond. Physiol Rev, 94(4):1287-1312.

[80]Polini A, Pisignano D, Parodi M, et al., 2011. Osteoinduction of human mesenchymal stem cells by bioactive composite scaffolds without supplemental osteogenic growth factors. PLoS ONE, 6(10):e26211.

[81]Purohit SD, Bhaskar R, Singh H, et al., 2019. Development of a nanocomposite scaffold of gelatin-alginate-graphene oxide for bone tissue engineering. Int J Biol Macromol, 133:592-602.

[82]Qian WY, Gong LQ, Cui X, et al., 2017. Nanotopographic regulation of human mesenchymal stem cell osteogenesis. ACS Appl Mater Interfaces, 9(48):41794-41806.

[83]Qian YZ, Zhou XF, Zhang FM, et al., 2019. Triple PLGA/PCL scaffold modification including silver impregnation, collagen coating, and electrospinning significantly improve biocompatibility, antimicrobial, and osteogenic properties for orofacial tissue regeneration. ACS Appl Mater Interfaces, 11(41):37381-37396.

[84]Re F, Sartore L, Moulisova V, et al., 2019. 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation. J Tissue Eng, 10:1-16.

[85]Rezvani Z, Venugopal JR, Urbanska AM, et al., 2016. A bird’s eye view on the use of electrospun nanofibrous scaffolds for bone tissue engineering: current state-of-the-art, emerging directions and future trends. Nanomedicine: NBM, 12(7):2181-2200.

[86]Ribba L, Parisi M, D'Accorso NB, et al., 2014. Electrospun nanofibrous mats: from vascular repair to osteointegration. J Biomed Nanotechnol, 10(12):3508-3535.

[87]Sankar S, Sharma CS, Rath SN, et al., 2018. Electrospun nanofibres to mimic natural hierarchical structure of tissues: application in musculoskeletal regeneration. J Tissue Eng Regen Med, 12(1):e604-e619.

[88]Sever M, Mammadov B, Guler MO, et al., 2014. Tenascin-C mimetic peptide nanofibers direct stem cell differentiation to osteogenic lineage. Biomacromolecules, 15(12):4480-4487.

[89]Shah S, Solanki A, Lee KB, 2016. Nanotechnology-based approaches for guiding neural regeneration. Acc Chem Res, 49(1):17-26.

[90]Shalumon KT, Sowmya S, Sathish D, et al., 2013. Effect of incorporation of nanoscale bioactive glass and hydroxyapatite in PCL/chitosan nanofibers for bone and periodontal tissue engineering. J Biomed Nanotechnol, 9(3):430-440.

[91]Shao WL, He JX, Sang F, et al., 2016. Enhanced bone formation in electrospun poly(L-lactic-co-glycolic acid)-tussah silk fibroin ultrafine nanofiber scaffolds incorporated with graphene oxide. Mater Sci Eng C, 62:823-834.

[92]Sun M, Spill F, Zaman MH, 2016. A computational model of YAP/TAZ mechanosensing. Biophys J, 110(11):2540-2550.

[93]Sun TW, Yu WL, Zhu YJ, et al., 2017. Hydroxyapatite nanowire @magnesium silicate core-shell hierarchical nanocomposite: synthesis and application in bone regeneration. ACS Appl Mater Interfaces, 9(19):16435-16447.

[94]Takada I, Kouzmenko AP, Kato S, 2009. Wnt and PPARγ signaling in osteoblastogenesis and adipogenesis. Nat Rev Rheumatol, 5(8):442-447.

[95]Tatapudy S, Aloisio F, Barber D, et al., 2017. Cell fate decisions: emerging roles for metabolic signals and cell morphology. EMBO Rep, 18(12):2105-2118.


[97]Tavakol S, Rasoulian B, Ramezani F, et al., 2019. Core and biological motif of self-assembling peptide nanofiber induce a stronger electrostatic interaction than BMP2 with BMP2 receptor 1A. Mater Sci Eng C, 101:148-158.

[98]Tutak W, Jyotsnendu G, Bajcsy P, et al., 2017. Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells. J Biomed Mater Res B Appl Biomater, 105(5):989-1001.

[99]Wang D, Jang J, Kim K, et al., 2019. “Tree to bone”: lignin/ polycaprolactone nanofibers for hydroxyapatite biomineralization. Biomacromolecules, 20(7):2684-2693.

[100]Wu J, Xie LL, Lin WZY, et al., 2017. Biomimetic nanofibrous scaffolds for neural tissue engineering and drug development. Drug Discov Today, 22(9):1375-1384.

[101]Xie CM, Sun HL, Wang KF, et al., 2017. Graphene oxide nanolayers as nanoparticle anchors on biomaterial surfaces with nanostructures and charge balance for bone regeneration. J Biomed Mater Res Part A, 105(5):1311-1323.

[102]Xing F, Li L, Zhou CC, et al., 2019. Regulation and directing stem cell fate by tissue engineering functional microenvironments: scaffold physical and chemical cues. Stem Cells Int, 2019:2180925.

[103]Xiong JH, Almeida M, O'Brien CA, 2018. The YAP/TAZ transcriptional co-activators have opposing effects at different stages of osteoblast differentiation. Bone, 112:1-9.

[104]Xue RY, Qian YN, Li LH, et al., 2017. Polycaprolactone nanofiber scaffold enhances the osteogenic differentiation potency of various human tissue-derived mesenchymal stem cells. Stem Cell Res Ther, 8:148.

[105]Yahia S, Khalil IA, El-Sherbiny IM, 2019. Sandwich-like nanofibrous scaffolds for bone tissue regeneration. ACS Appl Mater Interfaces, 11(32):28610-28620.

[106]Yang X, Li YY, He W, et al., 2018. Hydroxyapatite/collagen coating on PLGA electrospun fibers for osteogenic differentiation of bone marrow mesenchymal stem cells. J Biomed Mater Res A, 106(11):2863-2870.

[107]Yang ZQ, Si JH, Cui ZX, et al., 2017. Biomimetic composite scaffolds based on surface modification of polydopamine on electrospun poly(lactic acid)/cellulose nanofibrils. Carbohydr Polym, 174:750-759.

[108]Ye K, Cao LP, Li SY, et al., 2016. Interplay of matrix stiffness and cell–cell contact in regulating differentiation of stem cells. ACS Appl Mater Interfaces, 8(34):21903-21913.

[109]Zaidi SK, Sullivan AJ, Medina R, et al., 2004. Tyrosine phosphorylation controls Runx2-mediated subnuclear targeting of YAP to repress transcription. EMBO J, 23(4):790-799.

[110]Zhang K, Wang Y, Sun T, et al., 2018. Bioinspired surface functionalization for improving osteogenesis of electrospun polycaprolactone nanofibers. Langmuir, 34(50):15544-15550.

[111]Zhang S, Jiang GJ, Prabhakaran MP, et al., 2017. Evaluation of electrospun biomimetic substrate surface-decorated with nanohydroxyapatite precipitation for osteoblasts behavior. Mater Sci Eng C, 79:687-696.

[112]Zhang XH, Meng S, Huang Y, et al., 2015. Electrospun gelatin/ β-TCP composite nanofibers enhance osteogenic differentiation of BMSCs and in vivo bone formation by activating Ca2+-sensing receptor signaling. Stem Cells Int, 2015: 507154.

[113]Zhang YF, Fan W, Ma ZC, et al., 2010. The effects of pore architecture in silk fibroin scaffolds on the growth and differentiation of mesenchymal stem cells expressing BMP7. Acta Biomater, 6(8):3021-3028.

[114]Zhu JX, Cai Q, Zhang X, et al., 2013. Biological characteristics of mesenchymal stem cells grown on different topographical nanofibrous poly-L-lactide meshes. J Biomed Nanotechnol, 9(10):1757-1767.

[115]Zhu Y, Li DW, Zhang K, et al., 2017. Novel synthesized nanofibrous scaffold efficiently delivered hBMP-2 encoded in adenoviral vector to promote bone regeneration. J Biomed Nanotechnol, 13(4):437-446.

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