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On-line Access: 2021-02-07

Received: 2020-07-14

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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.2 P.136-145


High expression of FABP4 in colorectal cancer and its clinical significance

Author(s):  Yan ZHANG, Wenjia ZHANG, Min XIA, Zhujun XIE, Fangmei AN, Qiang ZHAN, Wenying TIAN, Tianyue ZHU

Affiliation(s):  Department of Gastroenterology, the Second Affiliated Hospital of Nantong University, Nantong 226001, China; more

Corresponding email(s):   xmzb1013@163.com

Key Words:  Fatty acid-binding protein 4 (FABP4), Colorectal cancer (CRC), Epithelial-mesenchymal transition

Yan ZHANG, Wenjia ZHANG, Min XIA, Zhujun XIE, Fangmei AN, Qiang ZHAN, Wenying TIAN, Tianyue ZHU. High expression of FABP4 in colorectal cancer and its clinical significance[J]. Journal of Zhejiang University Science B, 2021, 22(2): 136-145.

@article{title="High expression of FABP4 in colorectal cancer and its clinical significance",
author="Yan ZHANG, Wenjia ZHANG, Min XIA, Zhujun XIE, Fangmei AN, Qiang ZHAN, Wenying TIAN, Tianyue ZHU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T High expression of FABP4 in colorectal cancer and its clinical significance
%A Wenjia ZHANG
%A Min XIA
%A Zhujun XIE
%A Fangmei AN
%A Qiang ZHAN
%A Wenying TIAN
%A Tianyue ZHU
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 2
%P 136-145
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000366

T1 - High expression of FABP4 in colorectal cancer and its clinical significance
A1 - Yan ZHANG
A1 - Wenjia ZHANG
A1 - Min XIA
A1 - Zhujun XIE
A1 - Fangmei AN
A1 - Qiang ZHAN
A1 - Wenying TIAN
A1 - Tianyue ZHU
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 2
SP - 136
EP - 145
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000366

ObjectiveTo investigate the relationship between the fatty acid-binding protein 4 (FABP4) and colorectal cancer (CRC).
MethodsUsing an enzyme-linked immunosorbent assay (ELISA), we measured the expression of FABP4 in plasma of 50 patients who underwent surgery for CRC from October 2017 to May 2018 and 50 healthy controls. The content of the visceral fat area (VFA) as seen with abdominal computed tomography (CT) scanning was measured by ImageJ software. The expression levels of FABP4, E-cadherin, and Snail proteins in CRC and adjacent tissues were determined by immunohistochemistry.
ResultsThe mean concentration of plasma FABP4 of CRC patients was higher than that of the control group (22.46 vs. 9.82 ng/mL; P<0.05). The concentration of plasma FABP4 was related to the tumor, node, metastatis (TNM) stage and lymph node metastasis and was independent of age, body mass index (BMI), tumor size and location, and the degree of differentiation of CRC. The concentration of plasma FABP4 was positively correlated with high VFA and lipoprotein-a (LPA) (P<0.05); but it was not correlated with total cholesterol (TG), total triglyceride (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), or apolipoprotein AI (Apo-AI). The expression of FABP4 protein in CRC tissues was positively correlated with the degree of CRC differentiation, tumor stage, and lymph node metastasis. The level of FABP4 protein was negatively correlated with E-cadherin protein (r=-0.3292, P=0.0196) and positively correlated with Snail protein (r=0.5856, P<0.0001).
ConclusionsHigh LPA and VFA were risk factors for increased plasma FABP4 in CRC patients. FABP4 protein was highly expressed in CRC tissues and associated with TNM stage, differentiation, and lymph node metastasis of CRC. The level of FABP4 in CRC tissue was correlated with E-cadherin and Snail expression, suggesting that FABP4 may promote CRC progression related to epithelial-mesenchymal transition (EMT).


创新点:结直肠癌患者血浆FABP4浓度明显高于对照组(22.46ng/mL vs.9.82ng/mL,P<0.05)。血浆FABP4浓度与肿瘤、淋巴结、转移灶(TNM)分期和淋巴结转移有关,与年龄、身体质量指数(BMI)、肿瘤大小、部位和结直肠癌分化程度无关。血浆FABP4浓度与高内脏脂肪区(VFA)和脂蛋白a(LPA)呈正相关(P<0.05),与总胆固醇(TG)、总甘油三酯(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)以及载脂蛋白AI无相关性。FABP4蛋白在结直肠癌组织中的表达与大肠癌分化程度、肿瘤分期和淋巴结转移呈正相关。FABP4蛋白水平与E钙粘蛋白呈负相关(r=?0.3292,P=0.0196),与SNAIL蛋白呈正相关(r=0.5856,P<0.0001)。


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