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Journal of Zhejiang University SCIENCE B 1998 Vol.-1 No.-1 P.

http://doi.org/10.1631/jzus.B2000441


Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells


Author(s):  Guan WANG, Mingyue HAO, Qiong LIU, Yanlong JIANG, Haibin HUANG, Guilian YANG, Chunfeng WANG

Affiliation(s):  College of Veterinary Medicine, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of animal production and product quality safety of Ministry of Education, Jilin Agricultural University, Changchun130118, China; more

Corresponding email(s):   wangchunfeng@jlau.edu.cn, yangguilian@jlau.edu.cn

Key Words:  Oxidative stress, Apoptosis, HUVEC cells, Hydrogen peroxide


Guan WANG, Mingyue HAO, Qiong LIU, Yanlong JIANG, Haibin HUANG, Guilian YANG, Chunfeng WANG. Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .

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author="Guan WANG, Mingyue HAO, Qiong LIU, Yanlong JIANG, Haibin HUANG, Guilian YANG, Chunfeng WANG",
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%T Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells
%A Guan WANG
%A Mingyue HAO
%A Qiong LIU
%A Yanlong JIANG
%A Haibin HUANG
%A Guilian YANG
%A Chunfeng WANG
%J Journal of Zhejiang University SCIENCE B
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%D 1998
%I Zhejiang University Press & Springer
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T1 - Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells
A1 - Guan WANG
A1 - Mingyue HAO
A1 - Qiong LIU
A1 - Yanlong JIANG
A1 - Haibin HUANG
A1 - Guilian YANG
A1 - Chunfeng WANG
J0 - Journal of Zhejiang University Science B
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IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B2000441


Abstract: 
This study probed the protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in human umbilical vein cells (HUVEC). We constructed a new functional Lb. plantarum (NC8-pSIP409-alr-ACEIP) with a double-gene-labeled non-resistant screen as an expression vector. An MTT colorimetric assay was carried out to determine the cell viability of HUVEC cells following pretreatment with NC8-pSIP409-alr-ACEIP. Flow cytometry (FCM) was used to determine the apoptosis rate of HUVEC cells. Caspase-3/8/9 activity was also assayed and Western blotting was used to determine protein expression of Bcl-2, Bax, iNOS, gp91phox, Ang-II and ACE2, as well as corresponding indicators of oxidative stress, such as ROS, MMP, MDA and SOD. NC8-pSIP409-alr-ACEIP attenuated H2O2-induced cell death, as determined by the MTT assay. NC8-pSIP409-alr-ACEIP reduced apoptosis of HUVEC cells by FCM. In addition, NC8-pSIP409-alr-ACEIP decreased in the corresponding indicators of oxidative stress, such as iNOS, gp91phox, MDA and ROS, an increase in SOD expression and Bcl-2 / Bax ratio, a decrease in caspase-3/-9, and no significant change in caspase-8, which inhibited changes in permeability of the mitochondrial membrane and prevented the loss of transmembrane potential. Expression of the hypertension-related protein Ang-II protein in HUVEC protected by NC8-pSIP409-alr-ACEIP decreased and expression of ACE2 protein increased. These results suggested that NC8-pSIP409-alr-ACEIP protects against H2O2-induced injury in HUVEC cells. The mechanism for this effect is related to enhancement of antioxidant capacity, and apoptosis.

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