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Received: 2020-12-17

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Meilin CUI


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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.11 P.941-953


Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum

Author(s):  Meilin CUI, Yuchang MA, Youwei YU

Affiliation(s):  College of Food Science, Shanxi Normal University, Taiyuan 030031, China

Corresponding email(s):   cuimeilin1988@163.com

Key Words:  Ganoderma lucidum, Triterpenoid, Heme oxygenase-1 (HO-1)/carbon monoxide (CO) signaling, Transcriptome sequencing

Meilin CUI, Yuchang MA, Youwei YU. Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum[J]. Journal of Zhejiang University Science B, 2021, 22(11): 941-953.

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author="Meilin CUI, Yuchang MA, Youwei YU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum
%A Meilin CUI
%A Yuchang MA
%A Youwei YU
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 11
%P 941-953
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000818

T1 - Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum
A1 - Meilin CUI
A1 - Yuchang MA
A1 - Youwei YU
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 11
SP - 941
EP - 953
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2000818

Ganoderic triterpenoids (GTs) are the primary bioactive constituents of the Basidiomycotina fungus, Ganoderma lucidum. These compounds exhibit antitumor, anti-hyperlipidemic, and immune-modulatory pharmacological activities. This study focused on GT accumulation in mycelia of G. lucidum mediated by the heme oxygenase-1 (HO-1)/carbon monoxide (CO) signaling. Compared with the control, hemin (10 μmol/L) induced an increase of 60.1% in GT content and 57.1% in HO-1 activity. Moreover, carbon monoxide-releasing molecule-2 (CORM-2), CO donor, increased GT content by 56.0% and HO-1 activity by 18.1%. Zn protoporphyrin IX (ZnPPIX), a specific HO-1 inhibitor, significantly reduced GT content by 26.0% and HO-1 activity by 15.8%, while hemin supplementation reversed these effects. transcriptome sequencing showed that HO-1/CO could function directly as a regulator involved in promoting GT accumulation by regulating gene expression in the mevalonate pathway, and modulating the reactive oxygen species (ROS) and Ca2+ pathways. The results of this study may help enhance large-scale GT production and support further exploration of GT metabolic networks and relevant signaling cross-talk.




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