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Yinghua XU


Xuemei LU


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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.6 P.481-501


Spirulina platensis aqueous extracts ameliorate colonic mucosal damage and modulate gut microbiota disorder in mice with ulcerative colitis by inhibiting inflammation and oxidative stress

Author(s):  Jian WANG, Liqian SU, Lun ZHANG, Jiali ZENG, Qingru CHEN, Rui DENG, Ziyan WANG, Weidong KUANG, Xiaobao JIN, Shuiqing GUI, Yinghua XU, Xuemei LU

Affiliation(s):  Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of Life Science and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China; more

Corresponding email(s):   luxuemei@gdpu.edu.cn, xuyh@nifdc.org.cn

Key Words:  Spirulina platensis aqueous extracts, Ulcerative colitis, Oxidative stress, Inflammation, Antioxidant, Gut microbiota

Jian WANG, Liqian SU, Lun ZHANG, Jiali ZENG, Qingru CHEN, Rui DENG, Ziyan WANG, Weidong KUANG, Xiaobao JIN, Shuiqing GUI, Yinghua XU, Xuemei LU. Spirulina platensis aqueous extracts ameliorate colonic mucosal damage and modulate gut microbiota disorder in mice with ulcerative colitis by inhibiting inflammation and oxidative stress[J]. Journal of Zhejiang University Science B, 2022, 23(6): 481-501.

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author="Jian WANG, Liqian SU, Lun ZHANG, Jiali ZENG, Qingru CHEN, Rui DENG, Ziyan WANG, Weidong KUANG, Xiaobao JIN, Shuiqing GUI, Yinghua XU, Xuemei LU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Spirulina platensis aqueous extracts ameliorate colonic mucosal damage and modulate gut microbiota disorder in mice with ulcerative colitis by inhibiting inflammation and oxidative stress
%A Jian WANG
%A Liqian SU
%A Jiali ZENG
%A Qingru CHEN
%A Ziyan WANG
%A Weidong KUANG
%A Xiaobao JIN
%A Shuiqing GUI
%A Yinghua XU
%A Xuemei LU
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 6
%P 481-501
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100988

T1 - Spirulina platensis aqueous extracts ameliorate colonic mucosal damage and modulate gut microbiota disorder in mice with ulcerative colitis by inhibiting inflammation and oxidative stress
A1 - Jian WANG
A1 - Liqian SU
A1 - Lun ZHANG
A1 - Jiali ZENG
A1 - Qingru CHEN
A1 - Rui DENG
A1 - Ziyan WANG
A1 - Weidong KUANG
A1 - Xiaobao JIN
A1 - Shuiqing GUI
A1 - Yinghua XU
A1 - Xuemei LU
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 6
SP - 481
EP - 501
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100988

ulcerative colitis (UC) is a chronic and recurrent inflammatory bowel disease (IBD) that has become a major gastroenterologic problem during recent decades. Numerous complicating factors are involved in UC development such as oxidative stress, inflammation, and microbiota disorder. These factors exacerbate damage to the intestinal mucosal barrier. Spirulina platensis is a commercial alga with various biological activity that is widely used as a functional ingredient in food and beverage products. However, there have been few studies on the treatment of UC using S. platensis aqueous extracts (SP), and the underlying mechanism of action of SP against UC has not yet been elucidated. Herein, we aimed to investigate the modulatory effect of SP on microbiota disorders in UC mice and clarify the underlying mechanisms by which SP alleviates damage to the intestinal mucosal barrier. Dextran sulfate sodium (DSS) was used to establish a normal human colonic epithelial cell (NCM460) injury model and UC animal model. The mitochondrial membrane potential assay 3-‍‍(4,5-dimethylthiazol-2-yl)-2,‍5-diphenyltetrazolium bromide (MTT) and staining with Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) and Hoechst 33258 were carried out to determine the effects of SP on the NCM460 cell injury model. Moreover, hematoxylin and eosin (H&E) staining, transmission electron microscopy (TEM), enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qPCR), western blot, and 16S ribosomal DNA (rDNA) sequencing were used to explore the effects and underlying mechanisms of action of SP on UC in C57BL/6 mice. In vitro studies showed that SP alleviated DSS-induced NCM460 cell injury. SP also significantly reduced the excessive generation of intracellular reactive oxygen species (ROS) and prevented mitochondrial membrane potential reduction after DSS challenge. In vivo studies indicated that SP administration could alleviate the severity of DSS-induced colonic mucosal damage compared with the control group. Inhibition of inflammation and oxidative stress was associated with increases in the activity of antioxidant enzymes and the expression of tight junction proteins (TJs) post-SP treatment. SP improved gut microbiota disorder mainly by increasing antioxidant enzyme activity and the expression of TJs in the colon. Our findings demonstrate that the protective effect of SP against UC is based on its inhibition of pro-inflammatory cytokine overproduction, inhibition of DSS-induced ROS production, and enhanced expression of antioxidant enzymes and TJs in the colonic mucosal barrier.


方法:采用DSS建立正常肠上皮细胞(NCM460)损伤模型和UC动物模型。采用噻唑兰法(MTT)、细胞凋亡染色法(AnnexinV-FITC/PI)、细胞染色法(Hoechst 33258)和线粒体膜电位法(MMP)测定SP对NCM460细胞损伤模型的影响。并采用苏木精&伊红染色法(H&E)、透射电镜(TEM)、酶联免疫吸附测定法(ELISA)、实时荧光定量聚合酶链式反应(qPCR)、蛋白质印迹法(Western blot)和16S rDNA测序等方法,探讨SP对UC小鼠的结肠黏膜损伤和肠道菌群的影响及其潜在作用机制。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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