CLC number:
On-line Access: 2022-06-16
Received: 2022-01-27
Revision Accepted: 2022-05-27
Crosschecked: 2022-09-16
Cited: 0
Clicked: 1457
Citations: Bibtex RefMan EndNote GB/T7714
Xiajing LI, Yiyu ZHANG, Ning WANG, Zhaohu YUAN, Xiaojie CHEN, Qicong CHEN, Hui DENG, Xinxin TONG, Honglin CHEN, Yuyou DUAN, Yaming WEI. CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells[J]. Journal of Zhejiang University Science B, 2022, 23(9): 732-746.
@article{title="CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells",
author="Xiajing LI, Yiyu ZHANG, Ning WANG, Zhaohu YUAN, Xiaojie CHEN, Qicong CHEN, Hui DENG, Xinxin TONG, Honglin CHEN, Yuyou DUAN, Yaming WEI",
journal="Journal of Zhejiang University Science B",
volume="23",
number="9",
pages="732-746",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200048"
}
%0 Journal Article
%T CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells
%A Xiajing LI
%A Yiyu ZHANG
%A Ning WANG
%A Zhaohu YUAN
%A Xiaojie CHEN
%A Qicong CHEN
%A Hui DENG
%A Xinxin TONG
%A Honglin CHEN
%A Yuyou DUAN
%A Yaming WEI
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 9
%P 732-746
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200048
TY - JOUR
T1 - CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells
A1 - Xiajing LI
A1 - Yiyu ZHANG
A1 - Ning WANG
A1 - Zhaohu YUAN
A1 - Xiaojie CHEN
A1 - Qicong CHEN
A1 - Hui DENG
A1 - Xinxin TONG
A1 - Honglin CHEN
A1 - Yuyou DUAN
A1 - Yaming WEI
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 9
SP - 732
EP - 746
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200048
Abstract: BackgroundCircular RNAs (circRNAs) are covalently closed single-stranded RNAs with multiple biological functions. circRNA.0007127 is derived from the carbon catabolite repression 4-negative on TATA-less (CCR4-NOT) complex subunit 2 (CNOT2), which was found to regulate tumor cell apoptosis through caspase pathway.
MethodsPotential circRNA.0007127 target microRNAs (miRNAs) were analyzed by miRanda, TargetScan, and RNAhybrid software, and the miRNAs with binding sites for apoptosis-related genes were screened. The roles of circRNA.0007127 and its downstream target, microRNA (miR)-513a-5p, were validated by quantitative real-time polymerase chain reaction (qPCR), flow cytometry, mitochondrial membrane potential, immunofluorescence, western blot, and caspase-8 (CASP8) protein activity in vitro in H2O2-induced k-562 cells. The circRNA.0007127‒miR-513a-5p and CASP8‒miR-513a-5p interactions were verified by luciferase reporter assays.
ResultsSilencing circRNA.0007127 decreased cell apoptosis by inhibiting CASP8 pathway activation in k-562 cells. Compared with the control group, the expression of CASP8 was reduced by 50% and the 43-kD fragment of CASP8 protein was significantly reduced (P≤0.05). The luciferase reporting assay showed that circRNA.0007127 combined with miR-513a-5p or CASP8, with extremely significant differences (P≤0.001). The overexpression of miR-513a-5p inhibited the gene expression level of CASP8 in a human myeloid leukemia cell model (75% change) and the level of a 43-kD fragment of CASP8 protein (P≤0.01). The rescue experiment showed that cotransfection with circRNA.0007127 small-interfering RNA (siRNA) and the miR-513a-5p inhibitor increased CASP8 gene expression and the apoptosis rate, suggesting that the miR-513a-5p inhibitor is a circRNA.0007127 siRNA antagonist.
ConclusionscircRNA.0007127 regulates K-562 cell apoptosis through the miR-513a-5p/CASP8 axis, which can serve as a novel powerful molecular target for k-562 cells.
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