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Journal of Zhejiang University SCIENCE B 1998 Vol.-1 No.-1 P.

http://doi.org/10.1631/jzus.B2300928


Mitochondria derived from hESC-MSCs alleviate inflammatory response in HGFs


Author(s):  Bicong GAO, Chenlu SHEN, Kejia LV, Xuehui LI, Yongting ZHANG, Fan SHI, Hongyan DIAO, Hua YAO

Affiliation(s):  Department of Stomatology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   yaohua@zju.edu.cn, diaohy@zju.edu.cn

Key Words:  hESC-MSCs, Mitochondrial transfer, Inflammatory response, Mitochondrial dysfunction, Periodontal disease


Bicong GAO, Chenlu SHEN, Kejia LV, Xuehui LI, Yongting ZHANG, Fan SHI, Hongyan DIAO, Hua YAO. Mitochondria derived from hESC-MSCs alleviate inflammatory response in HGFs[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .

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publisher="Zhejiang University Press & Springer",
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%A Bicong GAO
%A Chenlu SHEN
%A Kejia LV
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%A Yongting ZHANG
%A Fan SHI
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%I Zhejiang University Press & Springer
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A1 - Fan SHI
A1 - Hongyan DIAO
A1 - Hua YAO
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Abstract: 
Periodontitis is a common oral disease caused by bacteria coupled with an excessive host immune response. Stem cell therapy can be a promising treatment strategy for periodontitis, but the relevant mechanism is complicated. This study aimed to explore the therapeutic potential of mitochondria from human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs) for the treatment of periodontitis. The gingival tissues of periodontitis patients are characterized by abnormal mitochondrial structure. Human gingival fibroblasts (HGFs) were exposed to 5 μg/mL of lipopolysaccharide (LPS) for 24 h to establish a cell injury model. Either when treated with hESC-MSCs or with mitochondria derived from hESC-MSCs, HGF showed a reduced expression of inflammatory genes, increased ATP levels, decreased ROS production, and enhanced mitochondrial function compared to the control. The average efficiency of isolated mitochondrial transfer by hESC-MSCs was determined to be 8.93%. Besides, a therapy of local mitochondrial injection in mice with LPS-induced periodontitis showed a reduction in inflammatory gene expression, as well as an increase in both mitochondrial number and aspect ratio in gingival tissues. In conclusion, our results indicate that mitochondria derived from hESC-MSCs can reduce the inflammatory response and improve mitochondrial function in HGFs, showcasing that the transfer of mitochondria between hESC-MSCs and HGFs serves as a potential mechanism underlying the stem cell therapeutic effect.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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