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Bicong GAO, Chenlu SHEN, Kejia LV, Xuehui LI, Yongting ZHANG, Fan SHI, Hongyan DIAO, Hua YAO. Mitochondria derived from hESC-MSCs alleviate inflammatory response in HGFs[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="Mitochondria derived from hESC-MSCs alleviate inflammatory response in HGFs",
author="Bicong GAO, Chenlu SHEN, Kejia LV, Xuehui LI, Yongting ZHANG, Fan SHI, Hongyan DIAO, Hua YAO",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2300928"
}
%0 Journal Article
%T Mitochondria derived from hESC-MSCs alleviate inflammatory response in HGFs
%A Bicong GAO
%A Chenlu SHEN
%A Kejia LV
%A Xuehui LI
%A Yongting ZHANG
%A Fan SHI
%A Hongyan DIAO
%A Hua YAO
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300928
TY - JOUR
T1 - Mitochondria derived from hESC-MSCs alleviate inflammatory response in HGFs
A1 - Bicong GAO
A1 - Chenlu SHEN
A1 - Kejia LV
A1 - Xuehui LI
A1 - Yongting ZHANG
A1 - Fan SHI
A1 - Hongyan DIAO
A1 - Hua YAO
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300928
Abstract: Periodontitis is a common oral disease caused by bacteria coupled with an excessive host immune response. Stem cell therapy can be a promising treatment strategy for periodontitis, but the relevant mechanism is complicated. This study aimed to explore the therapeutic potential of mitochondria from human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs) for the treatment of periodontitis. The gingival tissues of periodontitis patients are characterized by abnormal mitochondrial structure. Human gingival fibroblasts (HGFs) were exposed to 5 μg/mL of lipopolysaccharide (LPS) for 24 h to establish a cell injury model. Either when treated with hESC-MSCs or with mitochondria derived from hESC-MSCs, HGF showed a reduced expression of inflammatory genes, increased ATP levels, decreased ROS production, and enhanced mitochondrial function compared to the control. The average efficiency of isolated mitochondrial transfer by hESC-MSCs was determined to be 8.93%. Besides, a therapy of local mitochondrial injection in mice with LPS-induced periodontitis showed a reduction in inflammatory gene expression, as well as an increase in both mitochondrial number and aspect ratio in gingival tissues. In conclusion, our results indicate that mitochondria derived from hESC-MSCs can reduce the inflammatory response and improve mitochondrial function in HGFs, showcasing that the transfer of mitochondria between hESC-MSCs and HGFs serves as a potential mechanism underlying the stem cell therapeutic effect.
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