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On-line Access: 2024-12-03

Received: 2024-06-06

Revision Accepted: 2024-09-25

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Xiaofeng REN

https://orcid.org/0000-0003-4518-2058

Huimin WANG

https://orcid.org/0009-0001-6574-5156

Xiaoman LV

https://orcid.org/0009-0002-5779-0237

Yingzhou HU

https://orcid.org/0000-0001-7220-7607

Hao LI

https://orcid.org/0000-0002-3160-8719

Wenchao WANG

https://orcid.org/0000-0002-5021-8660

Dongdong QIN

https://orcid.org/0000-0002-2005-8408

Xiaoli FENG

https://orcid.org/0009-0003-8523-290X

Xintian HU

https://orcid.org/0000-0003-1216-4806

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Journal of Zhejiang University SCIENCE B 2024 Vol.25 No.11 P.972-995

http://doi.org/10.1631/jzus.B2400294


Establishment of a standardized daily behavior collection and analysis system for brain disease models of rhesus and cynomolgus monkeys and its application in autism spectrum disorder


Author(s):  Xiaofeng REN, Huimin WANG, Xiaoman LV, Yi ZHOU, Yingyin FAN, Yanjun YU, Christoph W. TURCK, Yuhui CHEN, Longbao LV, Yingzhou HU, Hao LI, Wenchao WANG, Dongdong QIN, Xiaoli FENG, Xintian HU

Affiliation(s):  National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Key Laboratory of Genetic Evolution & Animal Models, National Resource Center for Non-human Primates, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming650107, China; more

Corresponding email(s):   xthu@mail.kiz.ac.cn, virgo1985712@163.com, qindong108@163.com, wangwenchao@mail.kiz.ac.cn, lihao@mail.kiz.ac.cn, huyingzhou@mail.kiz.ac.cn

Key Words:  Monkey daily behavior ethogram, Monkey behavior collection protocol, Rhesus monkey, Cynomolgus monkey, Autism spectrum disorder (ASD)


Xiaofeng REN, Huimin WANG, Xiaoman LV, Yi ZHOU, Yingyin FAN, Yanjun YU, Christoph W. TURCK, Yuhui CHEN, Longbao LV, Yingzhou HU, Hao LI, Wenchao WANG, Dongdong QIN, Xiaoli FENG, Xintian HU. Establishment of a standardized daily behavior collection and analysis system for brain disease models of rhesus and cynomolgus monkeys and its application in autism spectrum disorder[J]. Journal of Zhejiang University Science B, 2024, 25(11): 972-995.

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author="Xiaofeng REN, Huimin WANG, Xiaoman LV, Yi ZHOU, Yingyin FAN, Yanjun YU, Christoph W. TURCK, Yuhui CHEN, Longbao LV, Yingzhou HU, Hao LI, Wenchao WANG, Dongdong QIN, Xiaoli FENG, Xintian HU",
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%A Xiaofeng REN
%A Huimin WANG
%A Xiaoman LV
%A Yi ZHOU
%A Yingyin FAN
%A Yanjun YU
%A Christoph W. TURCK
%A Yuhui CHEN
%A Longbao LV
%A Yingzhou HU
%A Hao LI
%A Wenchao WANG
%A Dongdong QIN
%A Xiaoli FENG
%A Xintian HU
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A1 - Yingyin FAN
A1 - Yanjun YU
A1 - Christoph W. TURCK
A1 - Yuhui CHEN
A1 - Longbao LV
A1 - Yingzhou HU
A1 - Hao LI
A1 - Wenchao WANG
A1 - Dongdong QIN
A1 - Xiaoli FENG
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DOI - 10.1631/jzus.B2400294


Abstract: 
Complex brain diseases seriously endanger human health, and early diagnostic biomarkers and effective treatments are currently lacking. Due to ethical constraints on human research, establishing monkey models is crucial to address these issues. With the rapid development of technology, transgenic monkey models of a range of brain diseases, especially autism spectrum disorder (ASD), have been successfully established. However, to establish practical and effective brain disease models and subsequently apply them to disease mechanism and treatment studies, there is still a lack of a standard tool, i.e., a system for collecting and analyzing the daily behaviors of brain disease model monkeys. Therefore, with the goal of undertaking a comprehensive and quantitative study of behavioral phenotypes, we established a standard daily behavior collection and analysis system, including behavioral data collection protocols and a monkey daily behavior ethogram (MDBE) for rhesus and cynomolgus monkeys, which are the most commonly used non-human primates in model construction. Then, we used ASD as an application example after referring to the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, Text Revision (DSM-5-TR), which is widely used in clinical disease diagnosis to obtain ASD core clinical symptoms. We then established a sub-ethogram (ASD monkey core behavior ethogram (MCBE-ASD)) specifically for quantitative assessment of the core clinical symptoms of an ASD monkey model based on MDBE. Subsequently, we demonstrated the high reproducibility of the system.

恒河猴和食蟹猴脑疾病模型的标准化日常行为采集和分析系统的建立及其在孤独症谱系障碍中的应用

任晓峰1,2,5,王慧敏3,吕小满4,周仪3,范颖尹1,2,5,余妍君1,2,5,Christoph W. TURCK1,2,陈雨慧3,吕龙宝1,胡英周1,2,李浩1,2,王文超1,2,秦冬冬4,冯晓丽3,胡新天1,2
1中国科学院昆明动物研究所模式动物表型与遗传研究国家重大科技基础设施(灵长类设施),遗传进化与动物模型全国重点实验室,国家非人灵长类实验动物资源库,中国昆明市,650107
2中国科学院昆明动物研究所云南省动物模型与人类疾病机理重点实验室,昆明动物研究所/香港中文大学生物资源与疾病分子机理联合实验室,中国昆明市,650201
3昆明医科大学基础医学院生理学系,中国昆明市,650500
4云南中医药大学中医药防治神经精神疾病重点实验室,中国昆明市,650500
5中国科学院大学,中国北京市,101408
摘要:复杂脑疾病严重危害人类健康,目前缺乏早期诊断生物标志物和有效的治疗方法。由于人类研究受到伦理的限制,猴模型的建立对解决这些问题至关重要。随着转基因技术的快速发展,一系列脑疾病,尤其是孤独症谱系障碍(ASD)的转基因猴模型已被成功建立。然而,要建立实用、有效的脑疾病模型,并将其应用于疾病机理和治疗研究中,目前还缺乏一个标准的工具,即脑疾病模型猴的日常行为采集和分析系统。因此,我们以行为表型的全面、定量研究为目标,针对模型构建中常用的恒河猴和食蟹猴,建立了一个标准的日常行为采集和分析系统,包括行为数据采集方法和猴日常行为目录(MDBE)。然后,我们以ASD为应用实例,参考广泛用于临床疾病和模型研究的《精神障碍诊断与统计手册》(第五版,修订版)(DSM-5-TR)中的ASD核心临床症状,基于MDBE建立了一个用于ASD猴模型核心临床症状定量评估的子行为目录(ASD猴核心行为目录(MCBE-ASD))。随后,我们通过实验数据证明了该系统的高度可重复性,并比较了不同恒河猴个体间行为模式的差异。

关键词:猴日常行为目录;猴行为采集方法;恒河猴;食蟹猴;孤独症谱系障碍(ASD)

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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