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CLC number: TP212.3

On-line Access: 2014-05-06

Received: 2013-10-16

Revision Accepted: 2014-03-06

Crosschecked: 2014-04-11

Cited: 6

Clicked: 3559

Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE C 2014 Vol.15 No.5 P.383-389


A micro-machined thin film electro-acoustic biosensor for detection of pesticide residuals

Author(s):  Jing-jing Wang, Wei-hui Liu, Da Chen, Yan Xu, Lu-yin Zhang

Affiliation(s):  Jinan Campus, Shandong University of Science and Technology, Jinan 250010, China; more

Corresponding email(s):   phychenda@163.com, x1y5@hotmail.com

Key Words:  Biosensors, Electro-acoustic resonator, Pesticide residues, Immunoreactions

Jing-jing Wang, Wei-hui Liu, Da Chen, Yan Xu, Lu-yin Zhang. A micro-machined thin film electro-acoustic biosensor for detection of pesticide residuals[J]. Journal of Zhejiang University Science C, 2014, 15(5): 383-389.

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publisher="Zhejiang University Press & Springer",

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%A Jing-jing Wang
%A Wei-hui Liu
%A Da Chen
%A Yan Xu
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T1 - A micro-machined thin film electro-acoustic biosensor for detection of pesticide residuals
A1 - Jing-jing Wang
A1 - Wei-hui Liu
A1 - Da Chen
A1 - Yan Xu
A1 - Lu-yin Zhang
J0 - Journal of Zhejiang University Science C
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.C1300289

Increasing awareness concerning food safety problems has been driving the search for simple and efficient biochemical analytical methods. In this paper, we develop a portable electro-acoustic biosensor based on a film bulk acoustic resonator for the detection of pesticide residues in agricultural products. A shear mode ZnO film bulk acoustic resonator with a micro-machining structure was fabricated as a mass-sensitive transducer for the real-time detection of antibody-antigen reactions in liquids. In order to obtain an ultra-low detection level, the artificial antigens were immobilized on the sensing surface of the resonator to employ a competitive format for the immunoassays. The competitive immunoreactions can be observed clearly through monitoring the frequency changes. The presence of pesticides was detected through the diminution of the frequency shift compared with the level without pesticides. The limit of detection for carbaryl (a widely used pesticide for vegetables and crops) is 2×10−10 M. The proposed device represents a potential alternative to the complex optical systems and electrochemical methods that are currently being used, and represents a significant opportunity in terms of simplicity of use and portability for on-site food safety testing.


研究目的:过量农药残留是目前食品安全中的重要问题之一。现有的标准色谱、质谱方法和酶联免疫等生物传感器无法进行高灵敏的、普及化的、在线和迅速的农药残留检测。本文基于微机电系统技术,提出基于薄膜体声波电声谐振器(film bulk acoustic resonator, FBAR)的高灵敏生物传感器,并将其应用于农药残留检测。对该传感器的灵敏性、特异性、可靠性等应用特性进行研究。
创新要点:将ZnO压电薄膜作为产生GHz频段体声波谐振的功能层,通过设计激励电极,实现了在水中具有高Q值和高灵敏度的剪切波模式质量敏感换能器。在器件表面组装具有特异敏感性的生物抗体,实现对待测物质的生物识别;采用基于竞争性免疫反应的检测方法,达到对典型农药2×10−10 M的检测极限。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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