Abstract: Toxicity regression equations and half lethal doses (LD₅₀) of LCNS treatment of O. furnacalis larvae at 24, 48, and 72 h are shown in Table 1. The LD₅₀ of LCNS (dissolved in phosphate buffered saline (PBS) solution containing 1% acetonitrile, pH=7.0) at 24, 48, and 72 h was 341.7, 112.1, and 16.3 mg/kg, respectively. These values were used as the toxic dose reference of drug-loaded nanoparticles. Our previous study suggested that increasing the LCNS dose from 10 to 400 mg/kg increased the mortality rate of free LCNS from 25% to 65%; a low dose of M₄/L (10 mg/kg) resulted in a higher mortality rate (70%) at 24 h (Xiao et al., 2022). Considering the easy photolysis of LCNS (Xiao et al., 2022), insecticidal experiments using 10 or 200 mg/kg LCNS or M₄/L in the diet of the O. furnacalis larvae with 12-h starvation were conducted under dark or light conditions to analyze the effect of light on larval mortality.

不同尺寸和表面修饰的介孔硅纳米颗粒负载三氯氟氰菊酯对玉米螟幼虫的杀虫机理

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