CLC number: R512.6
On-line Access: 2019-07-05
Received: 2018-08-30
Revision Accepted: 2019-02-27
Crosschecked: 2019-06-10
Cited: 0
Clicked: 7018
Reinhard H. Dennin, Jian-Er Wo. DNA sequences homologous to hepatitis C virus (HCV) in the extrachromosomal circular DNA in peripheral blood mononuclear cells of HCV-negative subjects[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1800453 @article{title="DNA sequences homologous to hepatitis C virus (HCV) in the extrachromosomal circular DNA in peripheral blood mononuclear cells of HCV-negative subjects", %0 Journal Article TY - JOUR
HCV阴性者外周血单核细胞的染色体外环状DNA中与HCV同源的DNA序列创新点:首次从HCV阴性者eccDNA中检测到HCV 5’-非编码区(5’-NCR)基因组RNA序列,验证了我们的假设: HCV同源DNA序列存在于人的外周血单核细胞的eccDNA组分中. 方法:用分离的eccDNA进行HCV特异的聚合酶链反应(PCR),采用核苷酸序列同源性搜索分析软件(BLASTn)对测序结果进行比对分析,并检测其甲基化模式. 结论:实验结果证实了我们的假设:即部分HCV 5’-NCR基因组RNA序列存在于外周血单核细胞的eccDNA组分.同时,甲基化分析结果显示了个体间的甲基化模式所代表的受遗传调控的表观遗传特征. 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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