CLC number: Q291
On-line Access: 2019-09-06
Received: 2018-12-18
Revision Accepted: 2019-05-14
Crosschecked: 2019-08-14
Cited: 0
Clicked: 3983
Hui Zhu, Yun Li, Mao-Xian Wang, Ju-Hong Wang, Wen-Xin Du, Fei Zhou. Analysis of cardiovascular disease-related NF-κB-regulated genes and microRNAs in TNFα-treated primary mouse vascular endothelial cells[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1800631 @article{title="Analysis of cardiovascular disease-related NF-κB-regulated genes and microRNAs in TNFα-treated primary mouse vascular endothelial cells", %0 Journal Article TY - JOUR
基于TNFα处理的小鼠原代血管内皮细胞鉴定心血管疾病相关的NF-κB调控的基因和microRNA创新点:构建心血管疾病相关的NF-κB调控网络. 方法:基于NF-κB转录活性差异的小鼠原代血管内皮细胞模型,采用基因芯片(Genechip)检测NF-κB调控的基因和microRNA.再通过实时荧光定量聚合酶链反应(qRT-PCR)和生物信息学方法进行差异基因和microRNA的筛选、验证、功能注释,从而发现与心血管疾病相关的NF-κB调控的基因和microRNA. 结论:在肿瘤坏死因子α(TNFα)处理的小鼠原代血管内皮细胞中:NF-κB调控77个基因,其中45个基因上调,32个基因下调.NF-κB还在TNFα处理的HeLa细胞中调控其中10个基因.通过qRT-PCR验证了NF-κB上调Egr1、Tnf和Btg2的表达.基因功能注释表明,许多NF-κB调节的基因聚类到经典的NF-κB参与的生物学过程.在TNFα处理的小鼠原代血管内皮细胞中还发现:NF-κB调控26个microRNA,其中21个上调,5个下调.进一步研究发现,7个NF-κB调控的microRNA还可能调控9个NF-κB调控的基因.最后通过检索数据库发现,5个NF-κB调控的基因和12个NF-κB调控的microRNA与心血管疾病相关.因此,本研究提升了对心血管疾病进展分子机制的理解. 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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