CLC number: R587.2
On-line Access: 2019-09-06
Received: 2018-12-31
Revision Accepted: 2019-05-04
Crosschecked: 2019-08-08
Cited: 0
Clicked: 3536
Zhi-Da Zhang, Hui Ren, Wei-Xi Wang, Geng-Yang Shen, Jin-Jing Huang, Mei-Qi Zhan, Jing-Jing Tang, Xiang Yu, Yu-Zhuo Zhang, De Liang, Zhi-Dong Yang, Xiao-Bing Jiang. IGF-1R/β-catenin signaling axis is involved in type 2 diabetic osteoporosis[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1800648 @article{title="IGF-1R/β-catenin signaling axis is involved in type 2 diabetic osteoporosis", %0 Journal Article TY - JOUR
IGF-1R/β-catenin信号通路在2型糖尿病性骨质疏松中的作用创新点:发现IGF-1R/β-catenin信号通路在DOP病理机制中起作用,可能是DOP潜在的治疗靶点. 方法:收集DOP患者血清,使用酶联免疫吸附测定(ELISA)法检测IGF-1R水平.DOP大鼠在4周高脂饲料喂养后给予链脲佐菌素建模,对照组大鼠在普通饲料喂养4周后再给予链脲佐菌素溶媒(柠檬酸钠缓冲液).应用双能X线吸收法、生物力学测试和苏木精-伊红(HE)染色法分别评估椎体骨量、骨强度和骨组织形态.使用实时定量聚合酶链反应(qRT-PCR)和蛋白印迹法(western blotting)测定IGF-1R、糖原合成酶激酶-3β(GSK-3β)和β-catenin表达及其蛋白磷酸化水平. 结论:DOP患者血清IGF-1R较对照组高.DOP大鼠骨量、压缩强度明显减小,HE染色显示DOP椎体骨组织形态明显受损,IGF-1R信使RNA(mRNA)表达上调,IGF-1R、GSK-3β和β-catenin蛋白磷酸化增加.由此可见,IGF-1R/β-catenin信号通路在DOP的病理机制中起作用,该发现将有利于后期DOP治疗靶点的开发. 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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