CLC number: S91
On-line Access: 2019-08-05
Received: 2019-01-16
Revision Accepted: 2019-04-24
Crosschecked: 2019-07-09
Cited: 0
Clicked: 3903
Sun-jian Lyu, Xue-mei Yuan, Hai-qi Zhang, Wei-da Shi, Xiao-ying Hang, Li Liu, Ying-lei Wu. Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1900027 @article{title="Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system", %0 Journal Article TY - JOUR
一株新的大口黑鲈弹状病毒(YH01)的分离、鉴定及其糖蛋白的杆状病毒表达系统的构建创新点:基于获得的大口黑鲈弹状病毒的全基因组序列,并利用杆状病毒表达系统获得了高丰度的病毒糖蛋白,可结合家蚕用于后续口服疫苗的研发. 方法:利用cDNA末端快速扩增法(RACE)及逆转录聚合酶链反应(RT-PCR)等技术获得了病毒的部分序列,拼接获得了病毒的全基因组序列.通过ClustalW比较该病毒与已知弹状病毒的3’ leader及5’ trailer序列差异,进一步利用MEGA软件比较该病毒全基因组序列与其他鱼类弹状病毒的差异,确定其系统进化树中的分支及地位.基于糖蛋白序列分析结果,利用杆状病毒表达载体构建该病毒的糖蛋白表达系统,感染昆虫卵巢细胞(SF9)后,借助荧光定量PCR(qRT-PCR)、免疫荧光及蛋白质印迹法(western blot)等技术确定病毒增殖及糖蛋白的表达情况. 结论:该大口黑鲈弹状病毒株全长11 526 bp,且与鳜鱼弹状病毒为同一分支,属于水泡病毒属.构建的病毒糖蛋白杆状病毒表达系统成功在SF9细胞中表达了高丰度的糖蛋白. 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
Reference[1]Alonso M, Leong JAC, 2013. Licensed DNA vaccines against infectious hematopoietic necrosis virus (IHNV). Recent Pat DNA Gene Seq, 7(1):62-65. [2]Amarasinghe GK, Bào Y, Basler CF, et al., 2017. Taxonomy of the order Mononegavirales: update 2017. Arch Virol, 162(8):2493-2504. [3]Anderson ED, Mourich DV, Fahrenkrug SC, et al., 1996. Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus. Mol Mar Biol Biotechnol, 5(2):114-122. [4]Assenberg R, Delmas O, Morin B, et al., 2010. Genomics and structure/function studies of Rhabdoviridae proteins involved in replication and transcription. Antiviral Res, 87(2):149-161. [5]Barr JN, Whelan SPJ, Wertz GW, 2002. Transcriptional control of the RNA-dependent RNA polymerase of vesicular stomatitis virus. Biochim Biophys Acta, 1577(2):337-353. [6]Bauer EF, 2013. Cascades of enemy release: impacts of an invasive species (Neogobius melanostomus) on the parasite communities of two native predators (Micropterus dolomieu and Micropterus salmoides). MS Thesis, University of New York College of Environmental Science and Forestry, Syracuse, New York, USA. [7]Castric J, Rasschaert D, Bernard J, 1984. Evidence of lyssaviruses among rhabdovirus isolates from the European EEL Anguilla Anguilla. Ann Inst Pasteur/Virol, 135(1):35-55. [8]Chen ZY, Lei XY, Zhang QY, 2012. The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus. Vet Microbiol, 157(3-4):264-275. [9]Compans RW, Klenk HD, Caliguiri LA, et al., 1970. Influenza virus proteins: I. Analysis of polypeptides of the virion and identification of spike glycoproteins. Virology, 42(4):880-889. [10]Dorson M, Torchy C, Chilmonczyk S, et al., 1984. A rhabdovirus pathogenic for perch, Perca fluviatilis L.: isolation and preliminary study. J Fish Dis, 7(3):241-245. [11]Emmenegger EJ, Kurath G, 2008. DNA vaccine protects ornamental koi (Cyprinus carpio koi) against North American spring viremia of carp virus. Vaccine, 26(50):6415-6421. [12]Fu XZ, Lin Q, Liang HR, et al., 2017. The biological features and genetic diversity of novel fish rhabdovirus isolates in China. Arch Virol, 162(9):2829-2834. [13]Galinier R, van Beurden S, Amilhat E, et al., 2012. Complete genomic sequence and taxonomic position of eel virus European X (EVEX), a rhabdovirus of European eel. Virus Res, 166(1-2):1-12. [14]Gao EB, Chen GF, 2018. Micropterus salmoides rhabdovirus (MSRV) infection induced apoptosis and activated interferon signaling pathway in largemouth bass skin cells. Fish Shellfish Immunol, 76:161-166. [15]Grabowska AK, Lipińska AD, Rohde J, et al., 2009. New baculovirus recombinants expressing Pseudorabies virus (PRV) glycoproteins protect mice against lethal challenge infection. Vaccine, 27(27):3584-3591. [16]Ho PY, Chen YC, Maekawa S, et al., 2018. Efficacy of recombinant protein vaccines for protection against Nocardia seriolae infection in the largemouth bass Micropterus salmoides. Fish Shellfish Immunol, 78:35-41. [17]Hoffmann B, Beer M, Schütze H, et al., 2005. Fish rhabdoviruses: molecular epidemiology and evolution. In: Fu ZF (Ed.), The World of Rhabdoviruses. Springer, Berlin, Heidelberg, p.81-117. [18]Kim CH, Johnson MC, Drennan JD, et al., 2000. DNA vaccines encoding viral glycoproteins induce nonspecific immunity and Mx protein synthesis in fish. J Virol, 74(15):7048-7054. [19]Ksenofontov AL, Badun GA, Fedorova NV, et al., 2008. Quantitation of the glycoprotein spike area on the surface of enveloped viruses. Mol Biol, 42(6):973-975. [20]Lei Y, Qi RR, Cui LB, et al., 2015. Diagnosis of rhabdovirus disease in juvenile largemouth bass Micropterus salmonides. J Dalian Fish Univ, 30(3):305-308 (in Chinese). [21]Lorenzen N, Lorenzen E, Einer-Jensen K, et al., 1998. Protective immunity to VHS in rainbow trout (Oncorhynchus mykiss, Walbaum) following DNA vaccination. Fish Shellfish Immunol, 8(4):261-270. [22]Lorenzoni M, Dörr AJM, Erra R, et al., 2002. Growth and reproduction of largemouth bass (Micropterus salmoides Lacépède, 1802) in Lake Trasimeno (Umbria, Italy). Fish Res, 56(1):89-95. [23]Luo X, Deng GC, Zhao CC, et al., 2013. Isolation and preliminary identification of rhabdovirus from Channa maculata cultured in pond. Acta Hydrobiol Sin, 37(4):620-625 (in Chinese). [24]Lv SJ, Lu BJ, Xu JH, et al., 2015. Immune response of peroxinectin of Chinese mitten crab Eriocheir sinensis to exterior stimulation. Dev Comp Immunol, 51(1):56-64. [25]Ma DM, Deng GC, Bai JJ, et al., 2013. A strain of Siniperca chuatsi rhabdovirus causes high mortality among cultured largemouth bass in south China. J Aquat Anim Health, 25(3):197-204. [26]Sakai K, Hagiwara K, Omatsu T, et al., 2015. Isolation and characterization of a novel rhabdovirus from a wild boar (Sus scrofa) in Japan. Vet Microbiol, 179(3-4):197-203. [27]Sano T, Nishimura T, Okamoto N, et al., 1976. Isolation of rhabdovirus from European eels (Anguilla anguilla) at Japanese port of entry. Fish Health News, 5:5-6. [28]Seo JY, Kim KH, Kim SG, et al., 2006. Protection of flounder against hirame rhabdovirus (HIRRV) with a DNA vaccine containing the glycoprotein gene. Vaccine, 24(7):1009-1015. [29]Serena MS, Geisler C, Metz GE, et al., 2013. Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs. Protein Expr Purif, 90(1):1-8. [30]Toth AM, Geisler C, Aumiller JJ, et al., 2011. Factors affecting recombinant Western equine encephalitis virus glycoprotein production in the baculovirus system. Protein Expr Purif, 80(2):274-282. [31]Walker PJ, Wang YH, Cowley JA, et al., 1994. Structural and antigenic analysis of the nucleoprotein of bovine ephemeral fever rhabdovirus. J Gen Virol, 75(8):1889-1899. [32]Xu XG, Wang ZS, Zhang Q, et al., 2011. Baculovirus surface display of E envelope glycoprotein of Japanese encephalitis virus and its immunogenicity of the displayed proteins in mouse and swine models. Vaccine, 29(4):636-643. [33]Xu XG, Wang ZS, Zhang Q, et al., 2012. Baculovirus as a PRRSV and PCV2 bivalent vaccine vector: baculovirus virions displaying simultaneously GP5 glycoprotein of PRRSV and capsid protein of PCV2. J Virol Methods, 179(2):359-366. [34]Zeng WW, Wang Q, Wang YY, 2013. Isolation and characterization of a rhabdovirus from snakehead fish (Ophicephalus striatus). J Fish China, 37(9):1416-1424 (in Chinese). [35]Zhang LJ, Li NQ, Lin Q, et al., 2018. An avirulent Micropterus salmoides rhabdovirus vaccine candidate protects Chinese perch against rhabdovirus infection. Fish Shellfish Immunol, 77:474-480. [36]Zhang S, Huang YH, Zhu JL, et al., 2018. Expression of hNeuritin protein in a baculovirus expression system and the analysis of its activity. Gene, 647:129-135. [37]Zhang XL, Xiao F, Li SM, et al., 2018. China Fishery Statistical Yearbook 2018. China Agriculture Press, Beijing, China, p.33 (in Chinese). [38]Zhang ZY, You Y, Meng FL, et al., 2011. A primary research of using silkworm larvae as substitutes of feed proteins. J Changsha Univ, 25(2):68-69 (in Chinese). Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou
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