CLC number: R562.2
On-line Access: 2019-09-06
Received: 2019-04-11
Revision Accepted: 2019-05-23
Crosschecked: 2019-08-08
Cited: 0
Clicked: 3614
Ning Zhang, Hong-Tao Lu, Rong-Jia Zhang, Xue-Jun Sun. Protective effects of methane-rich saline on mice with allergic asthma by inhibiting inflammatory response, oxidative stress and apoptosis[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1900195 @article{title="Protective effects of methane-rich saline on mice with allergic asthma by inhibiting inflammatory response, oxidative stress and apoptosis", %0 Journal Article TY - JOUR
甲烷生理盐水通过抗氧化、抗炎症和抗凋亡发挥对哮喘小鼠的保护作用创新点:通常我们认为甲烷生理盐水对人体并不发挥生理性的影响,但近年来涌现出的研究发现甲烷生理盐水可以发挥对多种脏器缺血再灌注损伤的保护作用.我们采用卵清蛋白刺激的小鼠哮喘模型,发现腹腔注射甲烷生理盐水的方式可以发挥对哮喘小鼠的保护作用,减轻哮喘小鼠氧化应激指标,缓解炎症和凋亡水平. 方法:通过卵清蛋白刺激诱导小鼠气道高反应性的方式建立小鼠哮喘模型,治疗组小鼠给予甲烷生理盐水腹腔注射.通过测量小鼠气道阻力指数(RI)和动态肺顺应性(Cdyn)来检测小鼠气道高反应性;通过苏木精-伊红染色法(H&E)检测小鼠肺组织形态学;对小鼠肺泡灌洗液进行细胞测量;通过酶联免疫吸附试验(ELISA)测定灌洗液和采集的血清中白介素4(IL-4)、IL-5、IL-13和肿瘤坏死因子(TNF-α);通过生物化学的方式检测氧化应激指标(如丙二醛(MDA)、超氧歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GSH)、髓过氧化物酶(MPO)和8羟基脱氧鸟苷(8-OHdG));通过蛋白免疫印迹实验、实时定量聚合酶链式反应(qRT-PCR)和生化检测试剂盒检测凋亡相关蛋白. 结论:甲烷生理盐水可以改善哮喘小鼠的气道功能,减少肺组织中浸润的炎性细胞.其保护作用有可能是通过甲烷抗氧化、抗炎和抗凋亡的生物学特性发挥的. 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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