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On-line Access: 2021-11-16
Received: 2021-02-24
Revision Accepted: 2021-05-24
Crosschecked: 0000-00-00
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Citations: Bibtex RefMan EndNote GB/T7714
Lisha ZHOU, Changsheng DONG, Zhuoming XU, Xinran WANG, Luyi ZHANG, Siyuan CHEN, Jiahao CHEN, Yingying ZHU. NEDD8-conjugating enzyme E2 UBE2F confers radiation resistance by protecting lung cancer cells from apoptosis[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2100170 @article{title="NEDD8-conjugating enzyme E2 UBE2F confers radiation resistance by protecting lung cancer cells from apoptosis", %0 Journal Article TY - JOUR
NEDD8结合酶(E2)UBE2F抑制肺癌细胞凋亡导致其放疗抵抗创新点:揭示灭活NEDD8结合酶(E2)UBE2F可以诱导凋亡蛋白NOXA介导的细胞凋亡,提高肺癌细胞的放疗敏感性。鉴于UBE2F在肺癌放射增敏治疗中的潜在作用,靶向UBE2F有望成为一种新型有效的肺癌放射增敏策略。 方法:利用免疫印迹检测UBE2F和NOXA的表达情况;用台盼蓝染色法检测放射和双氧水处理后肺癌细胞的存活率;通过裸鼠皮下瘤模型来评估动物体内UBE2F表达对肺癌细胞放疗敏感性的影响。 结论:放射或双氧水等氧化压力处理可以诱导UBE2F表达,而活性氧(ROS)清除剂可完全阻断UBE2F的表达上调。氧化压力诱导的UBE2F高表达促进NOXA降解,抑制细胞凋亡,进而导致肺癌细胞放疗抵抗。而灭活UBE2F可以促进NOXA蛋白积聚,促进细胞凋亡,显著提高肺癌细胞对放疗的敏感性。此外,TCGA癌症数据分析显示UBE2F在人肺腺癌中的过表达导致总体生存率较低,进一步提示UBE2F是潜在的放射增敏靶标。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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