CLC number:
On-line Access: 2022-04-11
Received: 2021-08-01
Revision Accepted: 2021-12-19
Crosschecked: 2022-04-19
Cited: 0
Clicked: 1390
Citations: Bibtex RefMan EndNote GB/T7714
Hao WEN, Xi JIANG, Wenqiang WANG, Minyu WU, Hongjin BAI, Cuiyun WU, Lirong SHEN. Comparative transcriptome analysis of candidate genes involved in chlorogenic acid biosynthesis during fruit development in three pear varieties of Xinjiang Uygur Autonomous Region[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2100673 @article{title="Comparative transcriptome analysis of candidate genes involved in chlorogenic acid biosynthesis during fruit development in three pear varieties of Xinjiang Uygur Autonomous Region", %0 Journal Article TY - JOUR
三种新疆梨果发育过程中与绿原酸生物合成相关候选基因的转录组比较分析创新点:明确了在三种新疆梨果发育过程中,与绿原酸含量变化趋势和绿原酸生物合成相关的差异基因及关键限制性酶。 方法:在鸭梨、库尔勒香梨和圆黄等三种梨果的不同发育阶段采集果实样品,经液相色谱法分析不同发育期果品的绿原酸含量和熊果苷含量差异,然后选择花后88、103和163天这三个绿原酸含量变化典型的时间点样品提取RNA,进行转录组测序,采取无参转录组学方法挖掘梨果中绿原酸生物合成途径的关键酶基因。 结论:绿原酸含量总体随着梨果的发育呈下降趋势。筛选得到10个与绿原酸生物合成相关的差异基因,分别为2个4CL基因、1个C4H基因、2个C3’H基因和5个HCT基因,其中绿原酸生物合成下游途径的C3’H和HCT是关键的限速酶基因。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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