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On-line Access: 2022-12-15
Received: 2022-03-07
Revision Accepted: 2022-07-19
Crosschecked: 2022-12-15
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Zhibiao BAI, Kai HU, Jiahuan YU, Yizhe SHEN, Chun CHEN. Macrophage migration inhibitory factor protects bone marrow mesenchymal stem cells from hypoxia/ischemia-induced apoptosis by regulating lncRNA MEG3[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2200110 @article{title="Macrophage migration inhibitory factor protects bone marrow mesenchymal stem cells from hypoxia/ischemia-induced apoptosis by regulating lncRNA MEG3", %0 Journal Article TY - JOUR
巨噬细胞迁移抑制因子通过调控长链非编码RNA MEG3保护骨髓间充质干细胞免受缺氧/缺血诱导的细胞凋亡1温州医科大学第一临床医学院,中国温州市,325006 2温州医科大学附属第一医院骨科,中国温州市,325006 目的:探讨巨噬细胞迁移抑制因子(MIF)在缺氧/缺血(H/I)环境下对骨髓间充质干细胞(BMSCs)凋亡的影响。 创新点:(1)阐明了长链非编码RNA(lncRNA)MEG3在H/I诱导的小鼠BMSCs凋亡中的作用;(2)发现MIF可以通过调节lncRNA MEG3/p53信号通路缓解H/I介导的BMSCs凋亡;(3)MIF的细胞保护作用还与激活Wnt/β-连环蛋白(β-catenin)信号通路和降低活性氧(ROS)水平相关。 方法:采用细胞计数试剂8(CCK-8)法检测H/I条件下BMSCs有或无MIF或异氰脲酸甘油三酯(TGIC)预处理对细胞活力的影响。利用含有lncRNAMEG3的质粒或β-catenin小干扰RNA过表达或下调相应基因,并使用TGIC预处理激活p53信号通路,然后进一步检测MIF对H/I环境下BMSCs的保护作用。 结果:CCK-8、蛋白质印迹法和流式细胞术检测结果显示:MIF预处理可显著降低细胞内的ROS水平,改善H/I诱导的BMSCs凋亡;LncRNA MEG3过表达时细胞核内β-catenin的含量明显减少,而p-p53的表达上调;当p53信号通路被激活,β-catenin的表达被抑制时,MIF对H/I环境下BMSCs的保护作用明显减弱。 结论:MIF通过下调lncRNA MEG3/p53信号通路、激活Wnt/β-catenin信号通路和降低ROS水平来保护BMSCs免受H/I诱导的凋亡。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
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