JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Molecular identification of clinical isolates in China

Author(s): Ting-ting Liu¹, Ke Zhang², Xun Zhou¹
Affiliation(s): 1. Department of Dermatology, Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;2. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Corresponding email(s): zhouxun123@sina.com
Key Words: Internal transcribed spacer (ITS), Diagnosis, , Taxonomy, Phylogeny

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Ting-ting Liu, Ke Zhang, Xun Zhou. Molecular identification of Sporothrix clinical isolates in China[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1300136

@article{title="Molecular identification of Sporothrix clinical isolates in China",
author="Ting-ting Liu, Ke Zhang, Xun Zhou",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B1300136"
}

%0 Journal Article
%T Molecular identification of Sporothrix clinical isolates in China
%A Ting-ting Liu
%A Ke Zhang
%A Xun Zhou
%J Journal of Zhejiang University SCIENCE B
%P 100-108
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B1300136"

TY - JOUR
T1 - Molecular identification of Sporothrix clinical isolates in China
A1 - Ting-ting Liu
A1 - Ke Zhang
A1 - Xun Zhou
J0 - Journal of Zhejiang University Science B
SP - 100
EP - 108
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B1300136"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: In this study, we investigated the molecular phylogeny of 64 clinical isolates which were identified as Sporothrix schenckii sensu lato by morphological identification. All of the strains were isolates from patients from several provinces in China. The phylogeny was inferred by DNA sequence analyses based on datasets of the ribosomal internal transcribed spacer (ITS) and a combined ITS and partial β-tubulin region. Reference sequences were retrieved from GenBank. Results showed that all of the isolates were clustered in a distinct clade with a type of Sporothrix globosa. Our analysis showed that S. globosa is the causal agent of the tested sporotrichosis in China, rather than S. schenckii that was generally believed to be the case. The existence of S. schenckii in China remains to be confirmed. This study improved our understanding of the distribution of the species in S. schenckii complex.

中国地区孢子丝菌临床分离株的分子鉴定

研究目的：明确中国地区孢子丝菌病的致病菌种。
创新方法：构建中国地区临床孢子丝菌系统进化树，用分子生物学方法对致病菌种进行重新鉴定。
研究手段：多基因测序和系统发育分析。
重要结论：球形孢子丝菌可能为中国地区孢子丝菌病的主要病原菌，申克孢子丝菌在中国地区是否存在尚需进一步验证。

关键词组：内含子转录间隔区；球形孢子丝菌；分类学；系统发育

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中国地区孢子丝菌临床分离株的分子鉴定

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References