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On-line Access: 2022-07-23
Received: 2021-12-22
Revision Accepted: 2022-05-27
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In vitro 3D malignant melanoma model for the evaluation of hypericin-loaded oil-in-water microemulsion in photodynamic therapy
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Hui L. Ma, Wanlu Li, Mian Wang, Laudemir C. Varanda, Janice R. Perussi, Y. Shrike Zhang & Emanuel Carrilho. In vitro 3D malignant melanoma model for the evaluation of hypericin-loaded oil-in-water microemulsion in photodynamic therapy[J]. Journal of Zhejiang University Science D, 2022, 5(4): 660-673.
@article{title="In vitro 3D malignant melanoma model for the evaluation of hypericin-loaded oil-in-water microemulsion in photodynamic therapy",
author="Hui L. Ma, Wanlu Li, Mian Wang, Laudemir C. Varanda, Janice R. Perussi, Y. Shrike Zhang & Emanuel Carrilho",
journal="Journal of Zhejiang University Science D",
volume="5",
number="4",
pages="660-673",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-022-00202-6"
}
%0 Journal Article
%T In vitro 3D malignant melanoma model for the evaluation of hypericin-loaded oil-in-water microemulsion in photodynamic therapy
%A Hui L. Ma
%A Wanlu Li
%A Mian Wang
%A Laudemir C. Varanda
%A Janice R. Perussi
%A Y. Shrike Zhang & Emanuel Carrilho
%J Journal of Zhejiang University SCIENCE D
%V 5
%N 4
%P 660-673
%@ 1869-1951
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-022-00202-6
TY - JOUR
T1 - In vitro 3D malignant melanoma model for the evaluation of hypericin-loaded oil-in-water microemulsion in photodynamic therapy
A1 - Hui L. Ma
A1 - Wanlu Li
A1 - Mian Wang
A1 - Laudemir C. Varanda
A1 - Janice R. Perussi
A1 - Y. Shrike Zhang & Emanuel Carrilho
J0 - Journal of Zhejiang University Science D
VL - 5
IS - 4
SP - 660
EP - 673
%@ 1869-1951
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-022-00202-6
Abstract: Advances in biomimetic three-dimensional (3D) melanoma models have brought new prospects of drug screening and disease modeling, since their physiological relevancy for recapitulating in vivo tumor architectures is more accurate than traditional two-dimensional (2D) cell culture. Gelatin methacryloyl (GelMA) is widely used as a tissue-engineered scaffold hydrogel for 3D cell culture. In the present study, an in vitro 3D malignant melanoma model based on GelMA was fabricated to evaluate the efficiency of hypericin (Hy)-loaded microemulsion (ME) in photodynamic therapy against melanoma. The ME was produced by the spontaneous emulsification method to enhance the bioavailability of Hy at tumor sites. Hy-loaded MEs were applied to a 3D malignant melanoma model made using 6% GelMA and the co-culture of B16F10 and Balb/c 3T3 cells, followed by crosslinking using violet light (403 nm). The observation revealed excellent cell viability and the presence of F-actin cytoskeleton network. Hy-loaded MEs exhibited higher phototoxicity and cell accumulation (about threefold) than free Hy, and the cells cultured in the 3D system displayed lower susceptibility (about 2.5-fold) than those in 2D culture. These findings indicate that the developed MEs are potential delivery carriers for Hy; furthermore, GelMA hydrogel-based modeling in polydimethylsiloxane (PDMS) molds is a user-friendly and cost-effective in vitro platform to investigate drug penetration and provide a basis for evaluating nanocarrier efficiency for skin cancer and other skin-related diseases.
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