JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.5 P.421-430

http://doi.org/10.1631/jzus.B2000771

Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system

Author(s): Rongbo ZHANG, Jin LIU, Bin XU, You WU, Shunli LIANG, Qiang YUAN
Affiliation(s): Department of Neurology, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310005, China; more
Corresponding email(s): yuanqiang0825@163.com
Key Words: Cornuside, Experimental autoimmune encephalomyelitis, Multiple sclerosis, Inflammation

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Abstract: The present study was conducted to clarify the therapeutic effect of cornuside on experimental autoimmune encephalomyelitis (EAE) and its influence on T helper 17 (Th17) cell and regulatory T (Treg) cell infiltration into the central nervous system. Rats were randomly placed into four treatment groups: control, EAE, EAE+cornuside, and EAE+prednisolone. The neurological function scores of rats were assessed daily. On the second day after EAE rats began to show neurological deficit symptoms, the four groups were treated with normal saline, normal saline, cornuside (150 mg/kg), and prednisolone (5 mg/kg), respectively. The treatment was discontinued after two weeks, and the spinal cord was obtained for hematoxylin and eosin (H&E) and luxol fast blue staining, as well as retinoic acid receptor-related orphan receptor γ (RORγ) and forkhead box protein P3 (Foxp3) immunohistochemical staining. Blood was collected for Th17 and Treg cell flow cytometry testing, and the serum levels of interleukin (IL)-17A, IL-10, transforming growth factor-β (TGF-β), IL-6, IL-23, and IL-2 were measured via enzyme-linked immunosorbent assay (ELISA). Compared with rats in the EAE group, rats in the EAE+cornuside and EAE+prednisolone groups began to recover from neurological deficits earlier, and had a greater degree of improvement of symptoms. Focal inflammation, demyelination, and RORγ-positive cell infiltration were reduced by cornuside or prednisolone treatment, whereas the Foxp3-positive cell numbers were not significantly different. Meanwhile, the number of Th17 cells and the IL-17A, IL-6, and IL-23 levels were lower in the blood after cornuside or prednisolone treatment, whereas the number of Treg cells or the levels of IL-10, TGF-β, and IL-2 were not markedly different. cornuside can alleviate symptoms of EAE neurological deficits through its anti-inflammatory and immunosuppressive effects, and Th17 cells may be one of its therapeutic targets.

山茱萸新苷通过抑制Th17细胞浸润中枢神经系统缓解实验性自身免疫性脑脊髓炎

目的：为了明确山茱萸新苷对实验性自身免疫性脑脊髓炎（EAE）的治疗作用，以及对Th17和Treg细胞浸润中枢神经系统（CNS）的影响。
创新点：本研究提示山茱萸新苷可以改善EAE大鼠的神经功能缺损症状，减轻EAE大鼠炎症浸润及脱髓鞘，并抑制Th17细胞浸润。
方法：使用豚鼠脊髓匀浆乳剂皮下注射Lewis大鼠诱导EAE，每天进行神经功能评分。待EAE大鼠开始出现神经功能缺损症状的第2天，4组大鼠（对照组、EAE组、EAE/山茱萸新苷组和EAE/泼尼松龙组）分别接受生理盐水、生理盐水、山茱萸新苷（150 mg/kg）、泼尼松龙（5 mg/kg）治疗，2周后停止治疗。对大鼠脊髓进行HE和LFB染色，以及RORγ和Foxp3免疫组化染色，并通过流式细胞检测血液中Th17和Treg细胞数量，用酶联免疫吸附法（ELISA）检测血清中白介素17A（IL-17A）、IL-10、转化生长因子β（TGF-β）、IL-6、IL-23和IL-2水平。
结论：山茱萸新苷可以缓解EAE症状，这可能通过抗炎抗免疫作用产生，而Th17细胞可能是其发挥作用的靶标之一。因此，山茱萸新苷存在治疗多发性硬化（MS）的潜在可能性。

关键词：山茱萸新苷；实验性自身免疫性脑脊髓炎；多发性硬化；炎症

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山茱萸新苷通过抑制Th17细胞浸润中枢神经系统缓解实验性自身免疫性脑脊髓炎

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