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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.6 P.743~748

http://doi.org/10.1631/jzus.2004.0743


Expression and proteolytic activity of calpain in lens epithelial cells of oxidative cataract


Author(s):  XU Wen, YAO Ke, SUN Zhao-hui, WANG Kai-jun, SHENTU Xing-chao

Affiliation(s):  Eye Center, Second Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310009, China

Corresponding email(s):   xlren@zju.edu.cn

Key Words:  Cataract, Lens epithelial cell, Hydrogen peroxide, Calpain, Calcium


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XU Wen, YAO Ke, SUN Zhao-hui, WANG Kai-jun, SHENTU Xing-chao. Expression and proteolytic activity of calpain in lens epithelial cells of oxidative cataract[J]. Journal of Zhejiang University Science A, 2004, 5(6): 743~748.

@article{title="Expression and proteolytic activity of calpain in lens epithelial cells of oxidative cataract",
author="XU Wen, YAO Ke, SUN Zhao-hui, WANG Kai-jun, SHENTU Xing-chao",
journal="Journal of Zhejiang University Science A",
volume="5",
number="6",
pages="743~748",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0743"
}

%0 Journal Article
%T Expression and proteolytic activity of calpain in lens epithelial cells of oxidative cataract
%A XU Wen
%A YAO Ke
%A SUN Zhao-hui
%A WANG Kai-jun
%A SHENTU Xing-chao
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 6
%P 743~748
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0743

TY - JOUR
T1 - Expression and proteolytic activity of calpain in lens epithelial cells of oxidative cataract
A1 - XU Wen
A1 - YAO Ke
A1 - SUN Zhao-hui
A1 - WANG Kai-jun
A1 - SHENTU Xing-chao
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 6
SP - 743
EP - 748
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0743


Abstract: 
Objective: To study the role of calpain in the mechanism of oxidative cataract through detecting the level of intracellular free Ca2+, the expression and proteolytic activity of calpain in the lens epithelial cells (LECs) of H2O2-induced cataract. Methods: Rat lenses were cultured in vitro and cataract was induced by H2O2. The level of intracellular free Ca2+was measured by fluorescence determination with fura-2/AM. The expression of m-calpain protein in LECs was detected with immunohistochemical method. The proteolytic activity in LECs was measured using a fluorogenic synthetic substrate. Results: There were significant differences of the level of intracellular free Ca2+ (P=0.001, 0.000, 0.000), the expression of m-calpain (P=0.001, 0.000, 0.000) and the proteolytic activity of calpain (P=0.001, 0.000, 0.000) between H2O2-induced and control group at 6, 12 and 24 h, respectively. Conclusions: H2O2 can increase intracellular free Ca2+, then enhance the expression and proteolytic activity of calpain which may play a role in the mechanism of oxidative cataract of rat.

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