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CLC number: R605.971

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Received: 2005-06-02

Revision Accepted: 2005-07-17

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.9 P.907~912

http://doi.org/10.1631/jzus.2005.B0907


Effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic shock


Author(s):  LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, FAN Yu-jing, WANG Qi, BAO De-guo

Affiliation(s):  Department of Emergency, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; more

Corresponding email(s):   yuanqiang609@sina.com

Key Words:  Shock, Hemorrhagic, Resuscitation, Apoptosis


LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, FAN Yu-jing, WANG Qi, BAO De-guo. Effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic shock[J]. Journal of Zhejiang University Science B, 2005, 6(9): 907~912.

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author="LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, FAN Yu-jing, WANG Qi, BAO De-guo",
journal="Journal of Zhejiang University Science B",
volume="6",
number="9",
pages="907~912",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0907"
}

%0 Journal Article
%T Effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic shock
%A LU Yuan-qiang
%A CAI Xiu-jun
%A GU Lin-hui
%A FAN Yu-jing
%A WANG Qi
%A BAO De-guo
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 9
%P 907~912
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0907

TY - JOUR
T1 - Effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic shock
A1 - LU Yuan-qiang
A1 - CAI Xiu-jun
A1 - GU Lin-hui
A1 - FAN Yu-jing
A1 - WANG Qi
A1 - BAO De-guo
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 9
SP - 907
EP - 912
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0907


Abstract: 
Objective: To observe the effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock and active bleeding was established in 32 SD (Sprague-Dawley) rats. The rats were randomly divided into control group, no fluid resuscitation group (NF group), controlled fluid resuscitation group (NS40 group) and rapid large scale fluid resuscitation group (NS80 group). Each group contained 8 rats. The curative effects were compared. At the same time, the apoptosis in liver, kidney, lung and small intestinal mucosa of survivors after hemorrhage and resuscitation was detected by light microscopy in HE (hematoxylin and eosin) stained tissue sections, flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Results: The survival rate of early fluid resuscitation (14/16) was markedly higher than that of NF group (3/8). There was some apoptosis in liver, kidney, lung and small intestinal mucosa of all survivors. Compared with NF and NS40 groups, the apoptosis of liver, kidney and small intestinal mucosa of NS80 group was obviously increased. Conclusions: Among three fluid resuscitation methods, controlled fluid resuscitation can obviously improve the early survival rate and the apoptosis of liver, kidney and small intestinal mucosa in rats with severe and uncontrolled hemorrhagic shock, and may benefit improvement of prognosis.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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