Full Text:   <2713>

CLC number: Q789; Q94

On-line Access: 

Received: 2005-10-08

Revision Accepted: 2006-05-10

Crosschecked: 0000-00-00

Cited: 11

Clicked: 4878

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.11 P.868-872


Species-specific SCAR markers for authentication of Sinocalycanthus chinensis

Author(s):  YE Qian, QIU Ying-xiong, QUO Yan-qi, CHEN Jian-xin, YANG Shu-zhen, ZHAO Ming-shui, FU Cheng-xin

Affiliation(s):  Laboratory of Systematic and Evolutionary Botany and Biodiversity, School of Life Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   cxfu@zju.edu.cn

Key Words:  Authentication, Diagnostic PCRs, ISSR, SCAR, Sinocalycanthus chinensis, Specie-specific

YE Qian, QIU Ying-xiong, QUO Yan-qi, CHEN Jian-xin, YANG Shu-zhen, ZHAO Ming-shui, FU Cheng-xin. Species-specific SCAR markers for authentication of Sinocalycanthus chinensis[J]. Journal of Zhejiang University Science B, 2006, 7(11): 868-872.

@article{title="Species-specific SCAR markers for authentication of Sinocalycanthus chinensis",
author="YE Qian, QIU Ying-xiong, QUO Yan-qi, CHEN Jian-xin, YANG Shu-zhen, ZHAO Ming-shui, FU Cheng-xin",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Species-specific SCAR markers for authentication of Sinocalycanthus chinensis
%A YE Qian
%A QIU Ying-xiong
%A QUO Yan-qi
%A CHEN Jian-xin
%A YANG Shu-zhen
%A ZHAO Ming-shui
%A FU Cheng-xin
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 11
%P 868-872
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0868

T1 - Species-specific SCAR markers for authentication of Sinocalycanthus chinensis
A1 - YE Qian
A1 - QIU Ying-xiong
A1 - QUO Yan-qi
A1 - CHEN Jian-xin
A1 - YANG Shu-zhen
A1 - ZHAO Ming-shui
A1 - FU Cheng-xin
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 11
SP - 868
EP - 872
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0868

Sinocalycanthus chinensis, an endangered species endemic to China, is cultivated as an ornamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. diagnostic PCRs were performed using the primer pair with the total DNAs of S. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chinensis in seedling market.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Bandana, D., Mahipal, S., 2003. Molecular detection of cashew husk (Anacardium occidentale) adulteration in market samples of dry tea (Camellia sinensis). Planta Med., 69(9):882-884.

[2] Camacho, F.J., Liston, A., 2001. Population structure and genetic diversity of Botrychium pumicola (Ophioglossaceae) based on ISSR. Am. J. Bot., 88(6):1065-1070.

[3] Doyle, J.J., 1991. DNA Protocols for Plants—CTAB Total DNA Isolation. In: Hewitt, G.M., Johnston, A. (Eds.), Molecular Techniques in Taxonomy. Springer-Verlag, Berlin, Germany, p.283-293.

[4] Elizabeth, A.S., Michael, D.C., Geunhwa, J., 2003. Development of species-specific SCAR markers in Bentgrass. Crop Sci., 43:345-349.

[5] Esselman, E.J., Li, J.Q., Crawford, D.J., Windus, J.L., Wolfe, A.D., 1999. Clonal diversity in the rare Calamagrostis porteri ssp. insperata (Placeae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Mol. Ecol., 8(3):443-443.

[6] Fu, L.G., 1992. Chinese Plant Red Book. Science Press, Beijing, China (in Chinese).

[7] Hernández, P., Martin, A., Dorado, G., 1999. Development of SCARs by direct sequencing of RAPD products: a practical tool for the introgression and marker-assisted selection of wheat. Mol. Breed., 5(3):245-253.

[8] Micheli, M.R., Bova, R., Pascale, E., D'Ambrosio, E., 1994. Reproducible DNA fingerprint with the random amplified polymorphic DNA (RAPD) method. Nucl. Acids Res., 22:1921-1922.

[9] Paran, I., Michelmore, R.W., 1993. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet., 85(8):985-993.

[10] Welsh, J., McClelland, M., 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucl. Acids Res., 18:7213-7218.

[11] Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S., 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res., 18:6531-6535.

[12] Zhang, R.H., Liu, H.E., 1998. Wax Shrubs in World (Calycanthaceae). China Science and Technology Press, Beijing, China (in Chinese).

[13] Zietkiewicz, E., Rafalski, A., Labuda, D., 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20(2):176-183.

[14] Zou, Y.P., Ge, S., Wang, X.D., 2001. Molecular Markers in Systemic and Evolutionary Botany. Science Press, Beijing, China (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE