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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.11 P.868~872

http://doi.org/10.1631/jzus.2006.B0868


Species-specific SCAR markers for authentication of Sinocalycanthus chinensis


Author(s):  YE Qian, QIU Ying-xiong, QUO Yan-qi, CHEN Jian-xin, YANG Shu-zhen, ZHAO Ming-shui, FU Cheng-xin

Affiliation(s):  Laboratory of Systematic and Evolutionary Botany and Biodiversity, School of Life Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   cxfu@zju.edu.cn

Key Words:  Authentication, Diagnostic PCRs, ISSR, SCAR, Sinocalycanthus chinensis, Specie-specific


YE Qian, QIU Ying-xiong, QUO Yan-qi, CHEN Jian-xin, YANG Shu-zhen, ZHAO Ming-shui, FU Cheng-xin. Species-specific SCAR markers for authentication of Sinocalycanthus chinensis[J]. Journal of Zhejiang University Science B, 2006, 7(11): 868~872.

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author="YE Qian, QIU Ying-xiong, QUO Yan-qi, CHEN Jian-xin, YANG Shu-zhen, ZHAO Ming-shui, FU Cheng-xin",
journal="Journal of Zhejiang University Science B",
volume="7",
number="11",
pages="868~872",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0868"
}

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%T Species-specific SCAR markers for authentication of Sinocalycanthus chinensis
%A YE Qian
%A QIU Ying-xiong
%A QUO Yan-qi
%A CHEN Jian-xin
%A YANG Shu-zhen
%A ZHAO Ming-shui
%A FU Cheng-xin
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 11
%P 868~872
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0868

TY - JOUR
T1 - Species-specific SCAR markers for authentication of Sinocalycanthus chinensis
A1 - YE Qian
A1 - QIU Ying-xiong
A1 - QUO Yan-qi
A1 - CHEN Jian-xin
A1 - YANG Shu-zhen
A1 - ZHAO Ming-shui
A1 - FU Cheng-xin
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 11
SP - 868
EP - 872
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0868


Abstract: 
Sinocalycanthus chinensis, an endangered species endemic to China, is cultivated as an ornamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. diagnostic PCRs were performed using the primer pair with the total DNAs of S. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chinensis in seedling market.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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