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Journal of Zhejiang University SCIENCE A 2007 Vol.8 No.2 P.171~175


Capillary method for measuring near-infrared spectra of microlitre volume liquids

Author(s):  YUAN Bo, MURAYAMA Koichi

Affiliation(s):  Department of Information Science and Optoelectronic Engineering, State Key Laboratory of Modern Optical Instrumentation, CNERC for Optical Instrument, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   yyylaw@263.net

Key Words:  Near-infrared (NIR) spectroscopy, Capillary, Cuvette cell, Numerical analysis

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YUAN Bo, MURAYAMA Koichi. Capillary method for measuring near-infrared spectra of microlitre volume liquids[J]. Journal of Zhejiang University Science A, 2007, 8(2): 171~175.

@article{title="Capillary method for measuring near-infrared spectra of microlitre volume liquids",
author="YUAN Bo, MURAYAMA Koichi",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Capillary method for measuring near-infrared spectra of microlitre volume liquids
%J Journal of Zhejiang University SCIENCE A
%V 8
%N 2
%P 171~175
%@ 1673-565X
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.A0171

T1 - Capillary method for measuring near-infrared spectra of microlitre volume liquids
A1 - YUAN Bo
A1 - MURAYAMA Koichi
J0 - Journal of Zhejiang University Science A
VL - 8
IS - 2
SP - 171
EP - 175
%@ 1673-565X
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.A0171

The present study theoretically explored the feasibility of the capillary method for measuring near-infrared (NIR) spectra of liquid or solution samples with microlitre volume, which was proposed in our previous studies. Lambert-Beer absorbance rule was applied to establish a model for the integral absorbance of capillary, which was then implemented in numerical analyses of the effects of capillary on various spectral features and dynamic range of absorption measurement. The theoretical speculations indicated that the capillary method might be used in NIR spectroscopy, which was further supported by the empirical data collected from our experiments by comparison between capillary NIR spectra of several organic solvents and cuvette cell NIR spectra.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Dou, X.M., Yuan, B., Zhao, H.Y., Yin, G.Z., Ozaki, Y., 2004. Generalized two-dimensional correlation spectroscopy: theory and applications in analytical field. Science in China Ser. B, 47(3):257-266.

[2] Hirschfeld, T., 1985. Lens and wedge absorption cells for FT-IR spectroscopy. Applied Spectroscopy, 39(3):426-430.

[3] Katsumoto, Y., Adachi, D., Sato, H., Ozaki, Y., 2002. Usefulness of a curve fitting method in the analysis of overlapping overtones and combinations of CH stretching modes. Journal of Near-Infrared Spectroscopy, 10:85-91.

[4] Maeda, H., Wang, Y., Ozaki, Y., Suzuki, M., Czarnecki, M.A., Iwahashi, M., 1999. A near-infrared study of hydrogen bonds in alcohols—comparison of chemometrics and spectral analysis. Chemometrics and Intelligent Laboratory Systems, 45(1-2):121-130.

[5] Murayama, K., Yuan, B., Tomida, M., Ozaki, Y., Era, S., 2003a. Capillary Near-infrared Spectroscopy for Microliter Solutions: Basic and Bio-medical Application. 11th International Conference on Near Infrared Spectroscopy, Cordoba, Spain.

[6] Murayama, K., Yuan, B., Ozaki, Y., Tomida, M., Era, S., 2003b. Near-infrared spectroscopy for liquids of microliter volume using capillaries with wall transmission. Analyst, 128(2):957-959.

[7] Noda, I., 2000. Determination of two-dimensional correlation spectra using the Hilbert transform. Applied Spectroscopy, 54(7):994-999.

[8] Ozaki, Y., Šasic, S., Jiang, J.H., 2001. How can we unravel complicated near infrared spectra?—Recent progress in spectral analysis methods for resolution enhancement and band assignments in the near infrared region. Journal of Near-Infrared Spectroscopy, 9:63-95.

[9] Scarff, M., Arnold, S.A., Harvey, L.M., McNeil, B., 2006. Near-infrared spectroscopy for bioprocess monitoring and control: current status and future trends. Critical Reviews in Biotechnology, 26(1):17-39.

[10] Siesler, H.W., Ozaki, Y., Kawata, S., Heise, H.W., 2002. Near-infrared Spectroscopy: Principles, Instruments, Applications. Wiley-VCH, Weinheim, p.75-84, 179-269.

[11] van der Greef, J., Smilde, A.K., 2005. Symbiosis of chemometrics and metabolomics: past, present, and future. Journal of Chemometrics, 19(5-7):376-386.

[12] Ward, K.R., Ivatury, R.R., Barbee, R.W., Terner, J., Pittman, R., Torres, I.P., Spiess, B., 2006. Near-infrared spectroscopy for evaluation of the trauma patient: A technology review. Resuscitation, 68(1):27-44.

[13] Wu, X.H., Chen, D.Z., 2004. Recent development of non-linear patrital least squares in chemometrics. Chinese Journal of Analytical Chemistry, 32(4):534-540 (in Chinese).

[14] Yuan, B., Dou, X.M., 2004. Near-infrared spectral studies of hydrogen-bond in water-methanol mixtures. Spectroscopy and Spectral Analysis, 24(11):1319-1322 (in Chinese).

[15] Yuan, B., Murayama, K., Wu, Y., Tsenkova, R., Dou, X.M., Era, S., Ozaki, Y., 2003. Temperature-dependent near-infrared spectra of bovine serum albumin in aqueous solution: spectral analysis by principal component analysis and evolving factor analysis. Applied Spectroscopy, 57(10):1223-1229.

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