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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.9 P.686~692


In vitro assessment of gastrointestinal viability of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaeroides

Author(s):  ZHOU Xu-xia, PAN Yuan-jiang, WANG Yan-bo, LI Wei-fen

Affiliation(s):  Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   wfli@zju.edu.cn

Key Words:  Photosynthetic bacteria, Probiotics, Primary culture, Intestinal epithelial cell, Oreochromis nilotica

ZHOU Xu-xia, PAN Yuan-jiang, WANG Yan-bo, LI Wei-fen. In vitro assessment of gastrointestinal viability of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaeroides[J]. Journal of Zhejiang University Science B, 2007, 8(9): 686~692.

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author="ZHOU Xu-xia, PAN Yuan-jiang, WANG Yan-bo, LI Wei-fen",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T In vitro assessment of gastrointestinal viability of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaeroides
%A ZHOU Xu-xia
%A PAN Yuan-jiang
%A WANG Yan-bo
%A LI Wei-fen
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 9
%P 686~692
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0686

T1 - In vitro assessment of gastrointestinal viability of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaeroides
A1 - ZHOU Xu-xia
A1 - PAN Yuan-jiang
A1 - WANG Yan-bo
A1 - LI Wei-fen
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 9
SP - 686
EP - 692
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0686

The objectives of this study were to assess the potential of two photosynthetic bacteria (PSB), Rhodopseudomonas palustris HZ0301 and Rhodobacter sphaeroides HZ0302, as probiotics in aquaculture. The viability of HZ0301 and HZ0302 in simulated gastric transit conditions (pH 2.0, pH 3.0 and pH 4.0 gastric juices) and in simulated small intestinal transit conditions (pH 8.0, with or without 0.3% bile salts) was tested. The effects of HZ0301 and HZ0302 on the viability and permeability of intestinal epithelial cell in primary culture of tilapias, Oreochromis nilotica, were also detected. All the treatments were determined with three replicates. The simulated gastric transit tolerance of HZ0301 and HZ0302 strains was pH-dependent and correspondingly showed lower viability at pH 2.0 after 180 min compared with pH 3.0 and pH 4.0. Both HZ0301 and HZ0302 were tolerant to simulated small intestine transit with or without bile salts in our research. Moreover, there was no significant difference (P>0.05) among three treatments including the control and the groups treated with HZ0301 or HZ0302 both in intestinal epithelial cell viability and membrane permeability, showing no cell damage. In summary, this study demonstrated that HZ0301 and HZ0302 had high capacity of upper gastrointestinal transit tolerance and were relatively safe for intestinal epithelial cells of tilapias.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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