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CLC number: R64

On-line Access: 2010-01-01

Received: 2009-09-06

Revision Accepted: 2009-11-27

Crosschecked: 2009-12-11

Cited: 35

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.1 P.10-16

http://doi.org/10.1631/jzus.B0900163


Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing


Author(s):  Tie-lian LIU, Jing-cheng MIAO, Wei-hua SHENG, Yu-feng XIE, Quan HUANG, Yun-bo SHAN

Affiliation(s):  Cell and Molecular Biology Institute, College of Medicine, Soochow University, Suzhou 215123, China; more

Corresponding email(s):   jcyang@suda.edu.cn

Key Words:  Regenerated silk fibroin film, Cytocompatibility, Cytotoxicity


Tie-lian LIU, Jing-cheng MIAO, Wei-hua SHENG, Yu-feng XIE, Quan HUANG, Yun-bo SHAN. Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing[J]. Journal of Zhejiang University Science B, 2010, 11(1): 10-16.

@article{title="Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing",
author="Tie-lian LIU, Jing-cheng MIAO, Wei-hua SHENG, Yu-feng XIE, Quan HUANG, Yun-bo SHAN",
journal="Journal of Zhejiang University Science B",
volume="11",
number="1",
pages="10-16",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0900163"
}

%0 Journal Article
%T Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing
%A Tie-lian LIU
%A Jing-cheng MIAO
%A Wei-hua SHENG
%A Yu-feng XIE
%A Quan HUANG
%A Yun-bo SHAN
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 1
%P 10-16
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900163

TY - JOUR
T1 - Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing
A1 - Tie-lian LIU
A1 - Jing-cheng MIAO
A1 - Wei-hua SHENG
A1 - Yu-feng XIE
A1 - Quan HUANG
A1 - Yun-bo SHAN
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 1
SP - 10
EP - 16
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900163


Abstract: 
Objective: To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility. Methods: The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor (VEGF) expression of ECV304 cells, and VEGF, angiopoietin-1 (Ang-1), platelet-derived growth factor (PDGF) and fibroblast growth factor 2 (FGF2) expression of WI-38 cells were assessed by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, viable cell counting, flow cytometry (FCM), and enzyme-linked immunosorbant assay (ELISA). Results: We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells. Conclusion: The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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