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CLC number: Q492.5

On-line Access: 2010-01-06

Received: 2010-05-11

Revision Accepted: 2010-07-23

Crosschecked: 2010-12-13

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.1 P.55-64

http://doi.org/10.1631/jzus.B1000174


Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats


Author(s):  Wei Zhang, Quan-wei Wei, Zheng-chao Wang, Wei Ding, Wei Wang, Fang-xiong Shi

Affiliation(s):  Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Corresponding email(s):   fxshi@njau.edu.cn

Key Words:  Nitric oxide synthase, Nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) pathway, Ovary, Neonatal rats, Prepuberty


Wei Zhang, Quan-wei Wei, Zheng-chao Wang, Wei Ding, Wei Wang, Fang-xiong Shi. Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats[J]. Journal of Zhejiang University Science B, 2011, 12(1): 55-64.

@article{title="Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats",
author="Wei Zhang, Quan-wei Wei, Zheng-chao Wang, Wei Ding, Wei Wang, Fang-xiong Shi",
journal="Journal of Zhejiang University Science B",
volume="12",
number="1",
pages="55-64",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000174"
}

%0 Journal Article
%T Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats
%A Wei Zhang
%A Quan-wei Wei
%A Zheng-chao Wang
%A Wei Ding
%A Wei Wang
%A Fang-xiong Shi
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 1
%P 55-64
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000174

TY - JOUR
T1 - Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats
A1 - Wei Zhang
A1 - Quan-wei Wei
A1 - Zheng-chao Wang
A1 - Wei Ding
A1 - Wei Wang
A1 - Fang-xiong Shi
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 1
SP - 55
EP - 64
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000174


Abstract: 
Objective: The present study is designed to investigate the cellular expressions and immunolocalizations of three different nitric oxide synthase (NOS) isoforms and the related nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) signaling pathway in the ovaries of neonatal and immature rats. Methods: The ovaries were obtained from ICR (Institute for Cancer Research) female Sprague-Dawley rats at postnatal days 1, 5, 7, 10, and 19. Then we carried out the histologic examination, immunohistochemistry, measurement of NOS activity, and modifications within the NO/cGMP pathway. Results: During postnatal days 1, 5, 7, 10, and 19, all three isoforms of NOS were mainly localized to the oocytes and expressed as a gradual increase in granulosa cells and theca cells within the growing follicle. The ovarian total NOS activities and NO levels were increased at postnatal days 7 and 10 compared with other days. Conclusions: Our findings suggest that the locally produced NO and the NO/NOS signaling systems are involved in the follicular development to puberty.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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