Full Text:   <2020>

CLC number: Q93

On-line Access: 2011-05-06

Received: 2010-09-11

Revision Accepted: 2010-10-31

Crosschecked: 2011-04-10

Cited: 27

Clicked: 3966

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.5 P.385-392


Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis

Author(s):  Jin-long Cui, Shun-xing Guo, Pei-gen Xiao

Affiliation(s):  Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China

Corresponding email(s):   sxguo2006@yahoo.com.cn

Key Words:  Agar diffusion method, Agarwood, Antimicrobial bioactivity, Antitumor bioactivity, Endophytic fungi

Jin-long Cui, Shun-xing Guo, Pei-gen Xiao. Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis[J]. Journal of Zhejiang University Science B, 2011, 12(5): 385-392.

@article{title="Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis",
author="Jin-long Cui, Shun-xing Guo, Pei-gen Xiao",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis
%A Jin-long Cui
%A Shun-xing Guo
%A Pei-gen Xiao
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 5
%P 385-392
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000330

T1 - Antitumor and antimicrobial activities of endophytic fungi from medicinal parts of Aquilaria sinensis
A1 - Jin-long Cui
A1 - Shun-xing Guo
A1 - Pei-gen Xiao
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 5
SP - 385
EP - 392
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000330

The purpose of this study was to isolate and characterize endophytic fungi from the stem tissue which can produce fragrant ingredients in Aquilaria sinensis (also called agarwood) to determine their antitumor and antimicrobial activities. Twenty-eight fungal endophytes were isolated from agarwood by strict sterile sample preparation and were classified into 14 genera and 4 taxonomic classes (Sordariomycetes, Dothideomycetes, Saccharomycetes, and Zygomycetes) based on molecular identification. Of the 28 isolates, 13 (46.4%) showed antimicrobial activity against at least one of the test strains by the agar well diffusion method, and 23 isolates (82.1%) displayed antitumor activity against at least one of five cancer cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The diameters of inhibition zones of YNAS07, YNAS14, HNAS04, HNAS05, HNAS08, and HNAS11 were equal to or higher than 14.0 mm against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, B. subtilis, Aspergillus fumigatus, and B. subtilis, respectively. The inhibition rates of YNAS06, YNAS08, and HNAS06 were not less than 60% to 293-T, 293-T, and SKVO3 cells, respectively. These results suggest that the endophytic fungi associated with agarwood will provide us with not only useful micro-ecological information, but also potential antimicrobial and antitumor agents.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Alley, M.C., Scudiero, D.A., Monks, A., Hursey, M.L., Czerwinski, M.J., Fine, D.L., 1988. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res., 48(3):589-601.

[2]Bacon, C.W., White, J.F., 2000. Microbial Endophytes. Marcel Dekker, New York, p.85-117.

[3]Gong, L.J., Guo, S.X., 2009. Endophytic fungi from Dracaena cambodiana and Aquilaria sinensis and their antimicrobial activity. Afr. J. Biotechnol., 8(5):731-736.

[4]Gunasekera, S.P., Kinghorn, A.D., Cordell, G.A., Farnsworth, N.R., 1981. Plant anticancer agents. XIX. Constituents of Aquilaria Malaccensis. J. Nat. Prod., 44(5):569-572.

[5]Guo, B., Wang, Y., Sun, X., Tang, K., 2008. Bioactive natural products from endophytes: a review. Appl. Biochem. Microbiol., 44(2):136-142.

[6]He, M.L., Qi, S.Y., Hu, L.J., 2005. Rapid in vitro propagation of medicinally important Aquilaria agallocha. J. Zhejiang Univ.-Sci. B, 6(8):849-852.

[7]Huang, W.Y., Cai, Y.Z., Hyde, K.D., Corke, H., Sun, M., 2007. Endophytic fungi from Nerium oleander L. (Apocynaceae): main constituents and antioxidant activity. World J. Microbiol. Biotechnol., 23(9):1253-1263.

[8]Kimura, M., 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol., 16(2):111-120.

[9]Kumar, S., Dudley, J., Nei, M., Tamura, K., 2008. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief. Bioinform., 9(4):299-306.

[10]Kusari, S., Zuhlke, S., Spiteller, M., 2009. An endophytic fungus from Camptotheca acuminata that produces camptothecin and analogues. J. Nat. Prod., 72(1):2-7.

[11]Li, J., Zhao, G.Z., Chen, H.H., Wang, H.B., Qin, S., Zhu, W.Y., Jiang, C.L., Li, W.J., 2008. Antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in rainfrest. Lett. Appl. Microbiol., 47(6):574-580.

[12]Liu, C.H., 1999. Chen-Xiang. In: Liu, C.H. (Ed.), Encyclopedia of Medicine of Li Shi Zhen, Ben Cao Gang Mu, Wood Part. Chinese Medicine publishing Inc., Beijing, p.1116-1118 (in Chinese).

[13]Lv, Y.L., Zhang, F.S., Chen, J., Cui, J.L., Xing, Y.M., Li, X.D., Guo, S.X., 2010. Diversity and antimicrobial activity of endophytic fungi associated with the alpine plant Saussurea involucrata. Biol. Pharm. Bull., 33(8):1300-1306.

[14]Mosmann, F., 1983. Rapid calorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J. Immunol. Methods, 65(1-2):55-63.

[15]Naruya, S., Masatoshi, N., 1987. The Neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol. Biol. Evol., 4(4):406-425.

[16]Okugawa, H., Ueda, R., Matsumoto, K., Kawanishi, K., Kato, A., 1993. Effects of agarwood extracts on the central nervous systems in mice. Planta Med., 59(1):32-36.

[17]Okugawa, H., Ueda, R., Matsumoto, K., Kawanishi, K., Kato, A., 1996. Effects of jinkoh-eremol and agarospirol from agarwood on the central nervous systems in mice. Planta Med., 62(1):2-6.

[18]Phongpaichit, S., Rungjindamai, N., Rukachaisirikul, N., Sakayaroj, J., 2006. Antimicrobial activity in cultures of endophytic fungi isolated from Garcinia species. FEMS Immunol. Med. Microbiol., 48(3):367-372.

[19]Qi, S.Y., 1995. III Aquilaria Species: In Vitro Culture and the Production of Eaglewood (Agarwood). In: Bajaji, Y.P.S. (Ed.), Biotechnology in Agriculture and Forestry. Medicine and Aromatic Plant VIII. Vol. 33, Springer-Verlag, Berlin Heidelberg, Germany, p.36-46.

[20]Rios, J.L., Recio, M.C., Villar, A., 1988. Screening methods for natural products with antimicrobial activity: a review of the literature. J. Ethnopharmacol., 23(2-3):127-149.

[21]Schulz, B., Boyle, C., 2005. The endophytic continuum. Mycol. Res., 109(6):661-686.

[22]Schulz, B., Wanke, U., Draeger, S., Aust, H.J., 1993. Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycol. Res., 97(12):1447-1450.

[23]Sieber, V.T., Hugentobler, C., 1987. Endophytic fungi in leaves and twigs of healthy and diseased beech trees (Fagus sylvatica L.). Eur. J. Forest Pathol., 17(7):411-425.

[24]Strobel, G.A., 2002. Rainforest endophytes and bioactive products. Crit. Rev. Biotechnol., 22(4):315-333.

[25]Tan, R.X., Zou, W.X., 2001. Endophytes: a rich source of functional metabolites. Nat. Prod. Rep., 18(4):448-459.

[26]Thompson, J.D., Higgins, D.G., Gibson, T.J., 1994. CLUSTAL W: improving the sensitivity of progressive multiple alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res., 22(22):4673-4680.

[27]Wang, L., Zhang, W.M., Pan, Q.L., Li, H.H., Tao, M.H., Gao, X.X., 2009. Isolation and molecular identification of endophytic fungi from Aquilaria sinensis. J. Fungal Res., 7(1):37-42 (in Chinese).

[28]Xu, L., Zhou, L., Zhao, J., Li, J., Li, X., Wang, J., 2008. Fungal endophytes from Dioscorea zingiberensis rhizomes and their antibacterial activity. Lett. Appl. Microbiol., 46(1):68-72.

[29]Yagura, T., Ito, M., Kiuchi, F., Honda, G., Shimada, Y., 2003. Four new 2-(2-phenylethyl) chromone derivatives from withered wood of Aquilaria sinensis. Chem. Pharm. Bull., 51(5):560-564.

[30]Yan, X.N., Sikora, I.R., Zheng, J.W., 2011. Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita. J. Zhejiang Univ.-Sci. B. (Biomed. & Biotechnol.), 12(3):219-225.

[31]Yang, J.S., 1998. Review of the chemical constituents isolated from Chen-Xiang. Nat. Prod. Res. Develop., 10(1):99-103 (in Chinese).

[32]Yang, J.S., Chen, Y.W., 1983. Studies on the constituents of Aquilaria sinensis (Lour.) Gilg. I. Isolation and structure elucidation of two new sesquiterpenes, baimuxinic acid and baimuxinal. Acta Pharm. Sin., 18(3):191-198 (in Chinese).

[33]Yang, J.S., Chen, Y.W., 1986. Studies on the chemical constituents of Aquilaria sinensis (Lour.) Gilg. II. Isolation and structure of baimuxinol and dehydrobaimuxinol. Acta Pharm. Sin., 21(7):516-520 (in Chinese).

[34]Yang, J.S., Wang, Y.L., Su, Y.L., 1989a. Studies on the chemical constituents of Aquilaria sinensis (Lour.) Gilg. IV. Isolation and characterization of 2-(2-phenylethyl) chromone derivatives. Acta Pharm. Sin., 24(9):678-683 (in Chinese).

[35]Yang, J.S., Wang, Y.L., Su, Y.L., He, C.H., Zheng, Q.T., Yang, J., 1989b. Studies on the chemical constituents of Aquilaria sinensis (Lour.) Gilg. III. Elucidation of the structure of isobaimuxinol and isolation and identification of the constituents of lower boiling fraction of the volatile oil. Acta Pharm. Sin., 24(4):264-268 (in Chinese).

[36]Yang, J.S., Wang, Y.L., Su, Y.L., 1990. Studies on the chemical constituents of Aquilaria sinensis (Lour.) Gilg. V. Isolation and characterization of three 2-(2-phenylethyl) chromone derivatives. Acta Pharm. Sin., 25(3):186-190 (in Chinese).

[37]Zhou, M.H., Wang, H.G., Suolang, J.B., Kou, J.P., Yu, B.Y., 2008. Antinociceptive and anti-inflammatory activities of Aquilaria sinensis (Lour.) Gilg. leaves extract. J. Ethnophamacol., 117(2):345-350.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE