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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.8 P.618-626

http://doi.org/10.1631/jzus.B1001008


Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment


Author(s):  Kang-ting Ji, Jun-de Chai, Cheng Xing, Jin-liang Nan, Peng-lin Yang, Ji-fei Tang

Affiliation(s):  Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical College, Wenzhou 325027, China

Corresponding email(s):   jefftang@medmail.com.cn

Key Words:  Danshen, Endothelial progenitor cells, Oxidized low-density lipoprotein, Hypercholesterolemia


Kang-ting Ji, Jun-de Chai, Cheng Xing, Jin-liang Nan, Peng-lin Yang, Ji-fei Tang. Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment[J]. Journal of Zhejiang University Science B, 2010, 11(8): 618-626.

@article{title="Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment",
author="Kang-ting Ji, Jun-de Chai, Cheng Xing, Jin-liang Nan, Peng-lin Yang, Ji-fei Tang",
journal="Journal of Zhejiang University Science B",
volume="11",
number="8",
pages="618-626",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1001008"
}

%0 Journal Article
%T Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment
%A Kang-ting Ji
%A Jun-de Chai
%A Cheng Xing
%A Jin-liang Nan
%A Peng-lin Yang
%A Ji-fei Tang
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 8
%P 618-626
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1001008

TY - JOUR
T1 - Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment
A1 - Kang-ting Ji
A1 - Jun-de Chai
A1 - Cheng Xing
A1 - Jin-liang Nan
A1 - Peng-lin Yang
A1 - Ji-fei Tang
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 8
SP - 618
EP - 626
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1001008


Abstract: 
In this study, we examined the protective effects of danshen both on endothelial progenitor cells (EPCs) in patients with hypercholesterolemia and on in-vitro EPCs of healthy volunteers. In the clinical study, we randomly divided 24 subjects with hypercholesterolemia into two groups (the control group and the danshen-treated group). At the end of two weeks of treatment, the EPC cellular functions of both groups were tested. The results indicated that, compared to the control group, EPCs in the danshen-treated group showed significantly better cellular functions, which was manifested in the cloning number, the proliferation capacity, the number of EPC adhesions, and cell migration. In the subsequent in-vitro experiments, EPCs were treated with vehicle, oxidized low-density lipoprotein (Ox-LDL, 100 μg/ml), or Ox-LDL (100 μg/ml) plus different concentrations of danshen (danshensu 2, 10, or 50 μg/ml, respectively) for 24 h. The results showed that danshen treatments can prevent the detrimental effects of Ox-LDL on EPC cellular functions measured by proliferation capacity (0.24±0.08, 0.37±0.11, 0.30±0.04 vs. 0.13±0.02, P<0.05, P<0.01, and P<0.01, respectively), and adhesion ability (63.00±11.60, 70.00±10.80, 85.50±11.41 vs. 40.50±6.85, all P<0.01). Compared to the group treated with Ox-LDL alone, danshen treatment significantly decreased the lipid peroxidation end product malondialdehyde (MDA) [(4.34±0.54), (3.98±0.47), (3.46±0.31) vs. (5.57±0.64) nmol/ml, all P<0.01], increased the production of superoxide dismutase (SOD) [(29.74±0.71), (31.09±0.83), (30.41±0.65) vs. (14.76±3.99) U/ml, all P<0.01], and lowered the expression of interleukin-6 (IL-6) [(24.62±7.69), (27.04±3.14), (33.38±18.86) vs. (230.67±33.53) pg/ml, all P<0.01] and tumor necrosis factor-α (TNF-α) [(41.72±6.10), (17.02±6.82), (3.73±2.26) vs. (228.71±41.53) pg/ml, all P<0.01] in Ox-LDL treated EPCs. These results suggest that danshen may exert a protective effect through its antioxidant and anti-inflammatory features.

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Reference

[1]Asahara, T., Murohara, T., Sullivan, A., Silver, M., van der Zee, R., Li, T., Witzenbichler, B., Schatteman, G., Isner, J.M., 1997. Isolation of putative progenitor endothelial cells for angiogenesis. Science, 275(5302):964-967.

[2]Baek, E.B., Yoo, H.Y., Park, S.J., Chung, Y.S., Hong, E.K., Kim, S.J., 2009. Inhibition of arterial myogenic responses by a mixed aqueous extract of Salvia miltiorrhiza and Panax notoginseng (PASEL) showing antihypertensive effects. Korean Journal of Physiology and Pharmacology, 13(4):287-293.

[3]Chen, J.Z., Zhang, F.R., Tao, Q.M., Wang, X.X., Zhu, J.H., Zhu, J.H., 2004. Number and activity of endothelial progenitor cells from peripheral blood in patients with hypercholesterolaemia. Clinical Science, 107(3):273-280.

[4]Du, G.H., Qiu, Y., Zhang, J.T., 2000. Salvianolic acid B protects the memory functions against transient cerebral ischemia in mice. Journal of Asian Natural Products Research, 2(2):145-152.

[5]Fish, J.M., Welchons, D.R., Kim, Y.S., Lee, S.H., Ho, W.K., Antzelevitch, C., 2006. Dimethyl Lithospermate B, an extract of Danshen, suppresses arrhythmogenesis associated with the brugada syndrome. Circulation, 113(11):1393-1400.

[6]Galle, J., Lehmann-Bodem, C., Hubner, U., Heinloth, A., Wanner, C., 2000. CyA and OxLDL cause endothelial dysfunction in isolated arteries through endothelinmediated stimulation of O(2)(−) formation. Nephrology Dialysis Transplantation, 15(3):339-346.

[7]Griese, D.P., Ehsan, A., Melo, L.G., Kong, D., Zhang, L., Mann, M.J., Pratt, R.E., Mulligan, R.C., Dzau, V.J., 2003. Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prosthetic grafts:implications for cell-based vascular therapy. Circulation, 108(21):2710-2715.

[8]Harrison, D.G., 1997. Endothelial function and oxidant stress. Clinical Cardiology, 20(11 Suppl. 2):II-11-17.

[9]Hill, J.M., Zalos, G., Halcox, J.P., Schenke, W.H., Waclawiw, M.A., Quyyumi, A.A., Finkel, T., 2003. Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. New England Journal of Medicine, 348(7):593-600.

[10]Ikeda, U., Ito, T., Shimada, K., 2001. Interleukin-6 and acute coronary syndrome. Clinical Cardiology, 24(11):701-704.

[11]Ji, K.T., Zhang, H.Q., Yang, P.L., 2006. Effects of compound salvia injection on number and activity of endothelial progenitor cells. China Journal of Chinese Materia Medica, 31(3):246-249 (in Chinese).

[12]Jiang, R.W., Lau, K.M., Hon, P.M., Mak, T.C., Woo, K.S., Fung, K.P., 2005. Chemistry and biological activities of caffeic acid derivatives from Salvia miltiorrhiza. Current Medicinal Chemistry, 12(2):237-246.

[13]Kawamoto, A., Gwon, C., Iwaguro, H., Yamaguchi, J.I., Uchida, S., Masuda, H., Silver, M., Ma, H., Kearney, M., Isner, J.M., et al., 2001. Therapeutic potential of ex vivo expanded endothelial progenitor cells for myocardial ischemia. Circulation, 103(5):634-637.

[14]Kawamoto, A., Tkebuchava, T., Yamaguchi, J., Nishimura, H., Yoon, Y.S., Milliken, C., Uchida, S., Masuo, O., Iwaguro, H., Ma, H., et al., 2003. Intramyocardial transplantation of autologous endothelial progenitor cells for therapeutic neovascularization of myocardial ischemia. Circulation, 107(3):461-468.

[15]Liu, C.L., Xie, L.X., Li, M., Durairajan, S.S., Goto, S., Huang, J.D., 2007. Salvianolic acid B inhibits hydrogen peroxide-induced endothelial cell apoptosis through regulating PI3K/Akt signaling. PLoS ONE, 2(12):e1321.

[16]Liu, H.B., Wen, J.K., Han, M., 2002. Effects of Salvia miltiorrhiza on matrix metalloproteinases and osteopontin gene expression and proliferation of cultured vascular smooth muscle cells. Chinese Journal of Integrated Traditional and Western Medicine, 22(10):764-766 (in Chinese).

[17]Murayama, T., Tepper, O.M., Silver, M., Ma, H., Losordo, D.W., Isner, J.M., Asahara, T., Kalka, C., 2002. Determination of bone marrow-derived endothelial progenitor cell significance in angiogenic growth factor-induced neovascularization in vivo. Experimental Hematology, 30(8):967-972.

[18]Pirro, M., Bagaglia, F., Paoletti, L., Razzi, R., Mannarino, M.R., 2008. Hypercholesterolemia-associated endothelial progenitor cell dysfunction. Therapeutic Advances in Cardiovascular Disease, 2(5):329-339.

[19]Rauscher, F.M., Goldschmidt-Clermont, P.J., Davis, B.H., Wang, T., Gregg, D., Ramaswami, P., Pippen, A.M., Annex, B.H., Dong, C., Taylor, D.A., 2003. Aging, progenitor cell exhaustion, and atherosclerosis. Circulation, 108(4):457-463.

[20]Ridker, P.M., Rifai, N., Pfeffer, M., Sacks, F., Lepage, S., Braunwald, E., 2000. Elevation of tumor necrosis factoralph and increased risk of recurrent coronary events after myocardial infarction. Circulation, 101(18):2149-2153.

[21]Ross, R., 1993. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature, 362(6423):801-809.

[22]Suzuki, T., Nishida, M., Futami, S., Fukino, K., Amaki, T., Aizawa, K., Chiba, S., Hirai, H., Maekawa, K., Nagai, R., 2003. Neoendothelialization after peripheral blood stemcell transplantation in humans: a case report of a Tokaimura nuclear accident victim. Cardiovascular Research, 58(2):487-492.

[23]Vasa, M., Fichtlscherer, S., Aicher, A., Adler, K., Urbich, C., Martin, H., Zeiher, A.M., Dimmeler, S., 2001. Number and migratory activity of circulating endothelial progenitor cells inversely correlate with risk factors for coronary artery disease. Circulation Research, 89(1):e1-e7.

[24]Walter, D.H., Rittig, K., Bahlmann, F.H., Kirchmair, R., Silver, M., Murayama, T., Nishimura, H., Losordo, D.W., Asahara, T., Isner, J.M., 2002. Statin therapy accelerate reendothelialization: a novel effect involving mobilization and incorporation of bone marrow-derived endothelial progenitor cells. Circulation, 105(25):3017-3024.

[25]Wang, X.X., Chen, J.Z., Tao, Q.M., Zhu, J.H., Shang, Y.P., 2004. Effects of Ox-LDL on number and activity of circulating endothelial progenitor cells. Drug and Chemical Toxicology, 27(3):243-255.

[26]Werner, N., Junk, S., Laufs, U., Link, A., Walenta, K., Bohm, M., Nickenig, G., 2003. Intravenous transfusion of endothelial progenitor cells reduces neointima formation after vascular injury. Circulation Research, 93(2):e17-e24.

[27]Wu, X.J., Wang, Y.P., 2002. Salvia magnesium acetate of oxidized low density lipoprotein-induced apoptosis of endothelial cells. Chinese Journal of Pathophysiology, 18(13):18-35 (in Chinese).

[28]Xu, D.B., Zhang, J.P., Zhang, W.Z., 2001. Experimental study on protective effect of Danshen for the cultured epithelial cells from injury by oxidized low density lipoprotein. Journal of Tianjin Medical University, 7(1):21-23 (in Chinese).

[29]Xu, Q., Zhang, Z., Davison, F., Hu, Y., 2003. Circulating progenitor cells regenerate endothelium of vein graft atherosclerosis, which is diminished in ApoE-deficient mice. Circulation Research, 93(8):e76-e86.

[30]Zhang, Z.X., Gao, F.Y., Li, K.M., 2004. Effect of magnesium lithospermate B on endothelial cells in human aorta after free radical injury. Chinese Journal of Integrated Traditional and Western Medicine, 24(6):521-524 (in Chinese).

[31]Zhou, L., Zuo, Z., Chow, M.S., 2005. Danshen: an overview of its chemistry, pharmacology, pharmacokinetics, and clinical use. The Journal of Clinical Pharmacology, 45(12):1345-1359.

[32]Zhu, J., Wang, X., Chen, J., Sun, J., Zhang, F., 2006. Reduced number and activity of circulating endothelial progenitor cells from patients with hyperhomocysteinemia. Archives of Medical Research, 37(4):484-489.

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JAING@JIANG<jiangsiqikaoyan@sina.com>

2010-08-09 19:51:28

I need to refer to this article, thank you

xie@qiang<xieqiang79@gmail.com>

2010-08-02 08:57:24

I need to refer to this article, thank you.

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