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On-line Access: 2010-08-02

Received: 2010-06-13

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.8 P.618-626


Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment

Author(s):  Kang-ting Ji, Jun-de Chai, Cheng Xing, Jin-liang Nan, Peng-lin Yang, Ji-fei Tang

Affiliation(s):  Department of Cardiology, the Second Affiliated Hospital, Wenzhou Medical College, Wenzhou 325027, China

Corresponding email(s):   jefftang@medmail.com.cn

Key Words:  Danshen, Endothelial progenitor cells, Oxidized low-density lipoprotein, Hypercholesterolemia

Kang-ting Ji, Jun-de Chai, Cheng Xing, Jin-liang Nan, Peng-lin Yang, Ji-fei Tang. Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment[J]. Journal of Zhejiang University Science B, 2010, 11(8): 618-626.

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author="Kang-ting Ji, Jun-de Chai, Cheng Xing, Jin-liang Nan, Peng-lin Yang, Ji-fei Tang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment
%A Kang-ting Ji
%A Jun-de Chai
%A Cheng Xing
%A Jin-liang Nan
%A Peng-lin Yang
%A Ji-fei Tang
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 8
%P 618-626
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1001008

T1 - Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment
A1 - Kang-ting Ji
A1 - Jun-de Chai
A1 - Cheng Xing
A1 - Jin-liang Nan
A1 - Peng-lin Yang
A1 - Ji-fei Tang
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 8
SP - 618
EP - 626
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1001008

In this study, we examined the protective effects of danshen both on endothelial progenitor cells (EPCs) in patients with hypercholesterolemia and on in-vitro EPCs of healthy volunteers. In the clinical study, we randomly divided 24 subjects with hypercholesterolemia into two groups (the control group and the danshen-treated group). At the end of two weeks of treatment, the EPC cellular functions of both groups were tested. The results indicated that, compared to the control group, EPCs in the danshen-treated group showed significantly better cellular functions, which was manifested in the cloning number, the proliferation capacity, the number of EPC adhesions, and cell migration. In the subsequent in-vitro experiments, EPCs were treated with vehicle, oxidized low-density lipoprotein (Ox-LDL, 100 μg/ml), or Ox-LDL (100 μg/ml) plus different concentrations of danshen (danshensu 2, 10, or 50 μg/ml, respectively) for 24 h. The results showed that danshen treatments can prevent the detrimental effects of Ox-LDL on EPC cellular functions measured by proliferation capacity (0.24±0.08, 0.37±0.11, 0.30±0.04 vs. 0.13±0.02, P<0.05, P<0.01, and P<0.01, respectively), and adhesion ability (63.00±11.60, 70.00±10.80, 85.50±11.41 vs. 40.50±6.85, all P<0.01). Compared to the group treated with Ox-LDL alone, danshen treatment significantly decreased the lipid peroxidation end product malondialdehyde (MDA) [(4.34±0.54), (3.98±0.47), (3.46±0.31) vs. (5.57±0.64) nmol/ml, all P<0.01], increased the production of superoxide dismutase (SOD) [(29.74±0.71), (31.09±0.83), (30.41±0.65) vs. (14.76±3.99) U/ml, all P<0.01], and lowered the expression of interleukin-6 (IL-6) [(24.62±7.69), (27.04±3.14), (33.38±18.86) vs. (230.67±33.53) pg/ml, all P<0.01] and tumor necrosis factor-α (TNF-α) [(41.72±6.10), (17.02±6.82), (3.73±2.26) vs. (228.71±41.53) pg/ml, all P<0.01] in Ox-LDL treated EPCs. These results suggest that danshen may exert a protective effect through its antioxidant and anti-inflammatory features.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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2010-08-09 19:51:28

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2010-08-02 08:57:24

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