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CLC number: R541.4

On-line Access: 2012-08-01

Received: 2012-06-15

Revision Accepted: 2012-06-26

Crosschecked: 2012-07-05

Cited: 19

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.8 P.616-623

10.1631/jzus.B1201004


Angiopoietin-1 preconditioning enhances survival and functional recovery of mesenchymal stem cell transplantation


Author(s):  Xian-bao Liu, Han Chen, Hui-qiang Chen, Mei-fei Zhu, Xin-yang Hu, Ya-ping Wang, Zhi Jiang, Yin-chuan Xu, Mei-xiang Xiang, Jian-an Wang

Affiliation(s):  Cardiovascular Key Lab of Zhejiang Province, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   Wang_jian_an@tom.com

Key Words:  Mesenchymal stem cells, Angiopoietin-1, Preconditioning, Survival, Myocardial infarction


Xian-bao Liu, Han Chen, Hui-qiang Chen, Mei-fei Zhu, Xin-yang Hu, Ya-ping Wang, Zhi Jiang, Yin-chuan Xu, Mei-xiang Xiang, Jian-an Wang. Angiopoietin-1 preconditioning enhances survival and functional recovery of mesenchymal stem cell transplantation[J]. Journal of Zhejiang University Science B, 2012, 13(8): 616-623.

@article{title="Angiopoietin-1 preconditioning enhances survival and functional recovery of mesenchymal stem cell transplantation",
author="Xian-bao Liu, Han Chen, Hui-qiang Chen, Mei-fei Zhu, Xin-yang Hu, Ya-ping Wang, Zhi Jiang, Yin-chuan Xu, Mei-xiang Xiang, Jian-an Wang",
journal="Journal of Zhejiang University Science B",
volume="13",
number="8",
pages="616-623",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1201004"
}

%0 Journal Article
%T Angiopoietin-1 preconditioning enhances survival and functional recovery of mesenchymal stem cell transplantation
%A Xian-bao Liu
%A Han Chen
%A Hui-qiang Chen
%A Mei-fei Zhu
%A Xin-yang Hu
%A Ya-ping Wang
%A Zhi Jiang
%A Yin-chuan Xu
%A Mei-xiang Xiang
%A Jian-an Wang
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 8
%P 616-623
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1201004

TY - JOUR
T1 - Angiopoietin-1 preconditioning enhances survival and functional recovery of mesenchymal stem cell transplantation
A1 - Xian-bao Liu
A1 - Han Chen
A1 - Hui-qiang Chen
A1 - Mei-fei Zhu
A1 - Xin-yang Hu
A1 - Ya-ping Wang
A1 - Zhi Jiang
A1 - Yin-chuan Xu
A1 - Mei-xiang Xiang
A1 - Jian-an Wang
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 8
SP - 616
EP - 623
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1201004


Abstract: 
Objective: Mesenchymal stem cell (MSC) transplantation is a promising therapy for ischemic heart diseases. However, poor cell survival after transplantation greatly limits the therapeutic efficacy of MSCs. The purpose of this study was to investigate the protective effect of angiopoietin-1 (Ang1) preconditioning on MSC survival and subsequent heart function improvement after transplantation. Methods: MSCs were cultured with or without 50 ng/ml Ang1 in complete medium for 24 h prior to experiments on cell survival and transplantation. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Hoechst staining were applied to evaluate MSC survival after serum deprivation in vitro, while cell survival in vivo was detected by terminal deoxynucleotidyl transferase biotin-dUPT nick end labeling (TUNEL) assay 24 and 72 h after transplantation. Heart function and infarct size were measured four weeks later by small animal echocardiography and Masson’s trichrome staining, respectively. Results: Ang1 preconditioning induced Akt phosphorylation and increased expression of Bcl-2 and the ratio of Bcl-2/Bax. In comparison with non-preconditioned MSCs, Ang1-preconditioned cell survival was significantly increased while the apoptotic rate decreased in vitro. However, the PI3K/Akt pathway inhibitor, LY294002, abrogated the protective effect of Ang1 preconditioning. After transplantation, the Ang1-preconditioned-MSC group showed a lower death rate, smaller infarct size, and better heart functional recovery compared to the non-preconditioned-MSC group. Conclusions: Ang1 preconditioning enhances MSC survival, contributing to further improvement of heart function.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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