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CLC number: R587.1

On-line Access: 2018-07-04

Received: 2017-05-13

Revision Accepted: 2017-07-03

Crosschecked: 2018-05-14

Cited: 0

Clicked: 1795

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Feng Zhou

https://orcid.org/0000-0002-8586-9922

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.7 P.559-569

http://doi.org/10.1631/jzus.B1700254


Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection


Author(s):  Xiao-xuan Guo, Yong Wang, Kai Wang, Bao-ping Ji, Feng Zhou

Affiliation(s):  Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; more

Corresponding email(s):   zf@cau.edu.cn

Key Words:  High-fat diet, Stability, Streptozotocin, Type 2 diabetes mellitus


Xiao-xuan Guo, Yong Wang, Kai Wang, Bao-ping Ji, Feng Zhou. Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection[J]. Journal of Zhejiang University Science B, 2018, 19(7): 559-569.

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author="Xiao-xuan Guo, Yong Wang, Kai Wang, Bao-ping Ji, Feng Zhou",
journal="Journal of Zhejiang University Science B",
volume="19",
number="7",
pages="559-569",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700254"
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%T Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection
%A Xiao-xuan Guo
%A Yong Wang
%A Kai Wang
%A Bao-ping Ji
%A Feng Zhou
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700254

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T1 - Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection
A1 - Xiao-xuan Guo
A1 - Yong Wang
A1 - Kai Wang
A1 - Bao-ping Ji
A1 - Feng Zhou
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 7
SP - 559
EP - 569
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700254


Abstract: 
Objective: The present study aims at determining the stability of a popular type 2 diabetes rat model induced by a high-fat diet combined with a low-dose streptozotocin injection. Methods: Wistar rats were fed with a high-fat diet for 8 weeks followed by a one-time injection of 25 or 35 mg/kg streptozotocin to induce type 2 diabetes. Then the diabetic rats were fed with regular diet/high-fat diet for 4 weeks. Changes in biochemical parameters were monitored during the 4 weeks. Results: All the rats developed more severe dyslipidemia and hepatic dysfunction after streptozotocin injection. The features of 35 mg/kg streptozotocin rats more resembled type 1 diabetes with decreased body weight and blood insulin. Rats with 25 mg/kg streptozotocin followed by normal diet feeding showed normalized blood glucose level and pancreatic structure, indicating that normal diet might help recovery from certain symptoms of type 2 diabetes. In comparison, diabetic rats fed with high-fat diet presented decreased but relatively stable blood glucose level, and this was significantly higher than that of the control group (P<0.05). Conclusions: This model easily recovers with normal diet feeding. A high-fat diet is suggested as the background diet in future pharmacological studies using this model.

高脂饮食联合低剂量链脲佐菌素造大鼠二型糖尿病模型稳定性的研究

目的:探讨高脂饮食联合低剂量链脲佐菌素(STZ)造大鼠二型糖尿病模型在不同饮食背景下的稳定性.
创新点:首次探讨在正常饮食和高脂饮食的背景下,该常见的大鼠二型糖尿病模型的稳定性.可为造模后期干预阶段的饲料选择提供依据.
方法:饲喂Wistar大鼠高脂饮食8周后,注射25或 35 mg/kg STZ来诱导二型糖尿病.之后每组分别饲喂正常饲料和高脂饲料4周,检测血液生化指标的稳定性.
结论:不管在何种饮食下,糖尿病大鼠都出现了空腹血糖显著恢复,血脂紊乱加剧的现象.其中35 mg/kg STZ注射组大鼠的特征更接近一型糖尿病,而 25 mg/kg STZ注射组大鼠表现出二型糖尿病的特征.相比高脂饮食,正常饮食更容易导致空腹血糖和胰岛结构的恢复.因此,在正常饮食下该模型稳定性欠佳,在后期的药理学实验中推荐使用高脂饮食.

关键词:高脂饮食;稳定性;链脲佐菌素;二型糖尿病

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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