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On-line Access: 2018-08-02

Received: 2017-07-07

Revision Accepted: 2017-10-02

Crosschecked: 2018-07-10

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Citations:  Bibtex RefMan EndNote GB/T7714


Chao-Yang Lin


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.8 P.610-619


Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance

Author(s):  Miao-Miao Liu, Xiao-Jing Zhang, Yan Gao, Zhi-Cheng Shen, Chao-Yang Lin

Affiliation(s):  Institute of Insect Science, Department of Plant Protection, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   chylin@zju.edu.cn

Key Words:  Transgenic maize, Bacillus thuringiensis (Bt), Insect resistance, Glyphosate tolerance

Miao-Miao Liu, Xiao-Jing Zhang, Yan Gao, Zhi-Cheng Shen, Chao-Yang Lin. Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance[J]. Journal of Zhejiang University Science B, 2018, 19(8): 610-619.

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%T Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance
%A Miao-Miao Liu
%A Xiao-Jing Zhang
%A Yan Gao
%A Zhi-Cheng Shen
%A Chao-Yang Lin
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700345

T1 - Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance
A1 - Miao-Miao Liu
A1 - Xiao-Jing Zhang
A1 - Yan Gao
A1 - Zhi-Cheng Shen
A1 - Chao-Yang Lin
J0 - Journal of Zhejiang University Science B
VL - 19
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%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1700345

A transgenic maize event ZD12-6 expressing a Bacillus thuringiensis (Bt) fusion protein Cry1Ab/Cry2Aj and a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein G10 was characterized and evaluated. Southern blot analysis indicated that ZD12-6 is a single copy integration event. The insert site was determined to be at chromosome 1 by border sequence analysis. Expression analyses of Bt fusion protein Cry1Ab/Cry2Aj and the EPSPS protein G10 suggested that they are both expressed stably in different generations. Insect bioassays demonstrated that the transgenic plants are highly resistant to Asian corn borer (Ostrinia furnacalis), cotton boll worm (Helicoverpa armigera), and armyworm (Mythimna separata). This study suggested that ZD12-6 has the potential to be developed into a commercial transgenic line.


方法:利用DNA印迹法(Southern blot)研究外源基因插入拷贝数;利用高效热不对称交错聚合酶链反应(hiTAIL-PCR)方法对外源基因的插入位点进行定位;通过蛋白质印迹法(Western blot)对外源蛋白进行定性分析;利用酶联免疫吸附测定(ELISA)分析外源蛋白表达量;通过生物测定对抗虫和草甘膦的抗性水平进行评估.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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