CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 0
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Citations: Bibtex RefMan EndNote GB/T7714
Congying ZHAO, Jinlong YANG, Hui XU, Shuyan MEI, Yating FANG, Qiong LAN, Yajun DENG, Bofeng ZHU. Genetic diversity analysis of forty-three insertion/deletion loci for forensic individual identification in Han Chinese from Beijing based on a novel panel[J]. Journal of Zhejiang University Science B, 2022, 23(3): 241-248.
@article{title="Genetic diversity analysis of forty-three insertion/deletion loci for forensic individual identification in Han Chinese from Beijing based on a novel panel",
author="Congying ZHAO, Jinlong YANG, Hui XU, Shuyan MEI, Yating FANG, Qiong LAN, Yajun DENG, Bofeng ZHU",
journal="Journal of Zhejiang University Science B",
volume="23",
number="3",
pages="241-248",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2100507"
}
%0 Journal Article
%T Genetic diversity analysis of forty-three insertion/deletion loci for forensic individual identification in Han Chinese from Beijing based on a novel panel
%A Congying ZHAO
%A Jinlong YANG
%A Hui XU
%A Shuyan MEI
%A Yating FANG
%A Qiong LAN
%A Yajun DENG
%A Bofeng ZHU
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 3
%P 241-248
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100507
TY - JOUR
T1 - Genetic diversity analysis of forty-three insertion/deletion loci for forensic individual identification in Han Chinese from Beijing based on a novel panel
A1 - Congying ZHAO
A1 - Jinlong YANG
A1 - Hui XU
A1 - Shuyan MEI
A1 - Yating FANG
A1 - Qiong LAN
A1 - Yajun DENG
A1 - Bofeng ZHU
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 3
SP - 241
EP - 248
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100507
Abstract: Due to the virtues of no stutter peaks, low rates of mutation, and short amplicon sizes, insertion/deletion (inDel) polymorphism is an indispensable tool for analyzing degraded DNA samples from crime scenes for human identifications (Wang et al., 2021). Herein, a self-developed panel of 43 inDel loci constructed previously by our group was utilized to evaluate the genetic diversities and explore the genetic background of the Han Chinese from Beijing (HCB) including 301 random healthy individuals. The lengths of amplicons at 43 inDel loci in this panel ranged from 87 to 199 bp, which indicated that the panel could be used as an effective tool to utilize highly degraded DNA samples for human identity testing. The loci in this panel were validated and performed well for forensic degraded DNA samples (Jin et al., 2021). The combined discrimination power (PD) and combined probability of exclusion (PE) values in this panel indicated that the 43 inDel loci could be used as the candidate markers in personal identification and parentage testing of HCB. In addition, population genetic relationships between the HCB and 26 reference populations from five continents based on 19 overlapped inDel loci were displayed by constructing a phylogenetic tree, principal component analysis (PCA), and population genetic structure analysis. The results illustrated that the HCB had closer genetic relationships with the Han populations from Chinese different regions.
[1]ChenQF, KangKL, SongJJ, et al., 2021. Allelic diversity and forensic estimations of the Beijing Hans: comparative data on sequence-based and length-based STRs. Forensic Sci Int Genet, 51:102424.
[2]DuWA, PengZY, FengCL, et al., 2017. Forensic efficiency and genetic variation of 30 InDels in Vietnamese and Nigerian populations. Oncotarget, 8(51):88934-88940.
[3]JiaJ, LiuX, FanQW, et al., 2021. Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes. Sci Rep, 11:609.
[4]JinR, CuiW, FangYT, et al., 2021. A novel panel of 43 insertion/deletion loci for human identifications of forensic degraded DNA samples: development and validation. Front Genet, 12:610540.
[5]ShenCM, ZhuBF, YaoTH, et al., 2016. A 30-InDel assay for genetic variation and population structure analysis of Chinese Tujia group. Sci Rep, 6:36842.
[6]SongF, LangM, LiLY, et al., 2020. Forensic features and genetic background exploration of a new 47-autosomal InDel panel in five representative Han populations residing in Northern China. Mol Genet Genom Med, 8(5):e1224.
[7]WangL, LvML, ZaumsegelD, et al., 2016. A comparative study of insertion/deletion polymorphisms applied among Southwest, South and Northwest Chinese populations using Investigator® DIPplex. Forensic Sci Int Genet, 21:10-14.
[8]WangMG, HeGL, GaoS, et al., 2021. Molecular genetic survey and forensic characterization of Chinese Mongolians via the 47 autosomal insertion/deletion marker. Genomics, 113(4):2199-2210.
[9]ZhaoYB, ZhangY, ZhangQC, et al., 2015. Ancient DNA reveals that the genetic structure of the northern Han Chinese was shaped prior to 3,000 years ago. PLoS ONE, 10(5):e0125676.
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