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On-line Access: 2022-12-15

Received: 2022-06-29

Revision Accepted: 2022-08-11

Crosschecked: 2022-12-15

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Shaohui ZONG

https://orcid.org/0000-0003-0868-6222

Gaofeng ZENG

https://orcid.org/0000-0002-0541-7388

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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.12 P.1002-1013

http://doi.org/10.1631/jzus.B2200344


Changes in the gut microbiota of osteoporosis patients based on 16S rRNA gene sequencing: a systematic review and meta-analysis


Author(s):  Rui HUANG, Pan LIU, Yiguang BAI, Jieqiong HUANG, Rui PAN, Huihua LI, Yeping SU, Quan ZHOU, Ruixin MA, Shaohui ZONG, Gaofeng ZENG

Affiliation(s):  College of Public Hygiene of Guangxi Medical University, Nanning 530021, China; more

Corresponding email(s):   xiaohui3008@126.com, fengfeng_388@126.com

Key Words:  Osteoporosis, Microbiome, Intestinal, 16S ribosomal RNA (rRNA) sequencing


Rui HUANG, Pan LIU, Yiguang BAI, Jieqiong HUANG, Rui PAN, Huihua LI, Yeping SU, Quan ZHOU, Ruixin MA, Shaohui ZONG, Gaofeng ZENG. Changes in the gut microbiota of osteoporosis patients based on 16S rRNA gene sequencing: a systematic review and meta-analysis[J]. Journal of Zhejiang University Science B, 2022, 23(12): 1002-1013.

@article{title="Changes in the gut microbiota of osteoporosis patients based on 16S rRNA gene sequencing: a systematic review and meta-analysis",
author="Rui HUANG, Pan LIU, Yiguang BAI, Jieqiong HUANG, Rui PAN, Huihua LI, Yeping SU, Quan ZHOU, Ruixin MA, Shaohui ZONG, Gaofeng ZENG",
journal="Journal of Zhejiang University Science B",
volume="23",
number="12",
pages="1002-1013",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200344"
}

%0 Journal Article
%T Changes in the gut microbiota of osteoporosis patients based on 16S rRNA gene sequencing: a systematic review and meta-analysis
%A Rui HUANG
%A Pan LIU
%A Yiguang BAI
%A Jieqiong HUANG
%A Rui PAN
%A Huihua LI
%A Yeping SU
%A Quan ZHOU
%A Ruixin MA
%A Shaohui ZONG
%A Gaofeng ZENG
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 12
%P 1002-1013
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200344

TY - JOUR
T1 - Changes in the gut microbiota of osteoporosis patients based on 16S rRNA gene sequencing: a systematic review and meta-analysis
A1 - Rui HUANG
A1 - Pan LIU
A1 - Yiguang BAI
A1 - Jieqiong HUANG
A1 - Rui PAN
A1 - Huihua LI
A1 - Yeping SU
A1 - Quan ZHOU
A1 - Ruixin MA
A1 - Shaohui ZONG
A1 - Gaofeng ZENG
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 12
SP - 1002
EP - 1013
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200344


Abstract: 
Backgroundosteoporosis (OP) has become a major public health issue, threatening the bone health of middle-aged and elderly people from all around the world. Changes in the gut microbiota (GM) are correlated with the maintenance of bone mass and bone quality. However, research results in this field remain highly controversial, and no systematic review or meta-analysis of the relationship between GM and OP has been conducted. This paper addresses this shortcoming, focusing on the difference in the GM abundance between OP patients and healthy controls based on previous 16S ribosomal RNA (rRNA) gene sequencing results, in order to provide new clinical reference information for future customized prevention and treatment options of OP.
MethodsAccording to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA), we comprehensively searched the databases of PubMed, Web of Science, Embase, Cochrane Library, and China National Knowledge Infrastructure (CNKI). In addition, we applied the R programming language version 4.0.3 and Stata 15.1 software for data analysis. We also implemented the Newcastle-Ottawa Scale (NOS), funnel plot analysis, sensitivity analysis, Egger’s test, and Begg’s test to assess the risk of bias.
ResultsThis research ultimately considered 12 studies, which included the fecal GM data of 2033 people (604 with OP and 1429 healthy controls). In the included research papers, it was observed that the relative abundance of Lactobacillus and Ruminococcus increased in the OP group, while the relative abundance for Bacteroides of Bacteroidetes increased (except for Ireland). Meanwhile, Firmicutes, Blautia, Alistipes, Megamonas, and Anaerostipes showed reduced relative abundance in Chinese studies. In the linear discriminant analysis Effect Size (LEfSe) analysis, certain bacteria showed statistically significant results consistently across different studies.
ConclusionsThis observational meta-analysis revealed that changes in the GM were correlated with OP, and variations in some advantageous GM might involve regional differences.

基于16S rRNA基因测序技术揭示骨质疏松症患者肠道菌群的改变:一项系统评价和荟萃分析

黄瑞1,刘槃2,4,白亦光2,5,黄洁琼1,潘瑞1,李惠华1,粟叶萍1,周佺3,马瑞鑫2,宗少晖2,曾高峰1
1广西医科大学公共卫生学院,广西南宁,530021
2广西医科大学第一附属医院脊柱骨科,广西南宁,530021
3广西南宁市第一人民医院创面修复科,广西南宁,530022
4新乡医学院第三附属医院骨科,河南新乡,453000
5川北医学院第二临床医学院,南充市中心医院骨科,四川南充,637000
概要:骨质疏松症(osteoporosis,OP)已成为严重威胁全球中老年人骨健康的重大公共卫生问题。肠道菌群(gut microbiota,GM)的改变与维持骨量和骨质量有关。然而,相关研究结果仍存在很大争议,目前尚没有研究对肠道菌群和骨质疏松间关系进行过系统评价和荟萃分析。本研究通过对骨质疏松症患者和健康人群之间肠道菌群差异的既往16S rRNA基因测序结果进行系统评价和荟萃分析,以期为未来个体化预防和治疗骨质疏松症提供新的临床参考。这项观察性的荟萃分析显示,肠道菌群的改变与骨质疏松症之间存在关联,并且一些优势菌群的变化可能存在地域差异。

关键词:骨质疏松症;微生物群;肠道;16S rRNA测序

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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