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On-line Access: 2023-06-13

Received: 2022-09-08

Revision Accepted: 2023-01-27

Crosschecked: 2023-07-21

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Chunmao HAN


Huawei SHAO


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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.6 P.510-523


Nanosilver alleviates foreign body reaction and facilitates wound repair by regulating macrophage polarization

Author(s):  Chuangang YOU, Zhikang ZHU, Shuangshuang WANG, Xingang WANG, Chunmao HAN, Huawei SHAO

Affiliation(s):  Department of Burns & Wound Care Center, the Second Affiliated Hospital, Zhejiang University School of Medicine,Hangzhou310009,China; more

Corresponding email(s):   2504131@zju.edu.cn, zrssk@zju.edu.cn

Key Words:  Nanosilver, Macrophages, Implants, Foreign body reaction, Wound repair

Chuangang YOU, Zhikang ZHU, Shuangshuang WANG, Xingang WANG, Chunmao HAN, Huawei SHAO. Nanosilver alleviates foreign body reaction and facilitates wound repair by regulating macrophage polarization[J]. Journal of Zhejiang University Science B, 2023, 24(6): 510-523.

@article{title="Nanosilver alleviates foreign body reaction and facilitates wound repair by regulating macrophage polarization",
author="Chuangang YOU, Zhikang ZHU, Shuangshuang WANG, Xingang WANG, Chunmao HAN, Huawei SHAO",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Nanosilver alleviates foreign body reaction and facilitates wound repair by regulating macrophage polarization
%A Chuangang YOU
%A Zhikang ZHU
%A Shuangshuang WANG
%A Xingang WANG
%A Chunmao HAN
%A Huawei SHAO
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 6
%P 510-523
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200447

T1 - Nanosilver alleviates foreign body reaction and facilitates wound repair by regulating macrophage polarization
A1 - Chuangang YOU
A1 - Zhikang ZHU
A1 - Shuangshuang WANG
A1 - Xingang WANG
A1 - Chunmao HAN
A1 - Huawei SHAO
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 6
SP - 510
EP - 523
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200447

foreign body reactions induced by macrophages often cause delay or failure of wound healing in the application of tissue engineering scaffolds. This study explores the application of nanosilver (NAg) to reduce foreign body reactions during scaffold transplantation. An NAg hybrid collagen-chitosan scaffold (NAg-CCS) was prepared using the freeze-drying method. The NAg-CCS was implanted on the back of rats to evaluate the effects on foreign body reactions. Skin tissue samples were collected for histological and immunological evaluation at variable intervals. Miniature pigs were used to assess the effects of NAg on skin wound healing. The wounds were photographed, and tissue samples were collected for molecular biological analysis at different time points post-transplantation. NAg-CCS has a porous structure and the results showed that it could release NAg constantly for two weeks. The NAg-CCS group rarely developed a foreign body reaction, while the blank-CCS group showed granulomas or necrosis in the subcutaneous grafting experiment. Both matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) were reduced significantly in the NAg-CCS group. The NAg-CCS group had higher interleukin (IL)-10 and lower IL-6 than the blank CCS group. In the wound healing study, M1 macrophage activation and inflammatory-related proteins (inducible nitric oxide synthase (iNOS), IL-6, and interferon-‍γ (IFN-‍γ)) were inhibited by NAg. In contrast, M2 macrophage activation and proinflammatory proteins (arginase-1, major histocompatibility complex-II (MHC-II), and found in inflammatory zone-1 (FIZZ-1)) were promoted, and this was responsible for suppressing the foreign body responses and accelerating wound healing. In conclusion, dermal scaffolds containing NAg suppressed the foreign body reaction by regulating macrophages and the expression of inflammatory cytokines, thereby promoting wound healing.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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