Journal of Zhejiang University

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Vascularized organoid-on-a-chip for centimeter-scale organoid cultivation

Author(s): Xiaofeng Gong (龚晓峰), Chen Yang (杨辰), Jianchen Peng (彭见晨), Xiao Ding (丁笑), Hui Yang (杨晖), Aaron Gerald Wang (王睿诚), Emmanuel Enoch Dzakah & Bing Zhao (赵冰)
Affiliation(s): State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China; School of Basic Medical Sciences, The First Affiliated Hospital of Nanchang University, Jiangxi Medical College, Nanchang University, Nanchang 330031, China; BMF Nano Material Technology Company Limited, Shenzhen 518017, China; Institute of Organoid Technology, Kunming Medical University, Kunming 650500, China; Shanghai American School, Shanghai 201100, China; Z Lab, bioGenous BIOTECH, Shanghai 200438, China
Corresponding email(s): bingzhao@ncu.edu.cn
Key Words: Organoid · Tumoroid · Biomaterials · Organoid-on-a-chip · Biomedical engineering

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Xiaofeng Gong (龚晓峰), Chen Yang (杨辰), Jianchen Peng (彭见晨), Xiao Ding (丁笑), Hui Yang (杨晖), Aaron Gerald Wang (王睿诚), Emmanuel Enoch Dzakah & Bing Zhao (赵冰). Vascularized organoid-on-a-chip for centimeter-scale organoid cultivation[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/bdm.2400424

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author="Xiaofeng Gong (龚晓峰), Chen Yang (杨辰), Jianchen Peng (彭见晨), Xiao Ding (丁笑), Hui Yang (杨晖), Aaron Gerald Wang (王睿诚), Emmanuel Enoch Dzakah & Bing Zhao (赵冰)",
journal="Journal of Zhejiang University Science B",
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publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/bdm.2400424"
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A1 - Xiao Ding (丁笑)
A1 - Hui Yang (杨晖)
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Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: An organoid is a three-dimensional (3D) cell culture model that can reproduce the distinct structure and inherent functionality of certain organs. Nevertheless, a major limitation of organoids is the absence of a complex vascular network, thus restricting the supply of oxygen and essential nutrients. Coupled with their inherent size constraints and metabolite accumulation, it is challenging for organoids to replicate the natural intricacies of organs, thereby limiting their applicability. To overcome the challenges associated with this technology, we developed a culture platform to cultivate tumors or organ-derived organoids up to the centimeter scale. Initially, a customized organoid-on-a-chip including a microvascular network at the micron scale was designed using 3D printing. Further, by integrating an infusion device, the chip ensures an adequate supply of nutrients and fluid immersion while mimicking blood flow dynamics. Our method overcomes the issue of the limited size of organ oids due to insufficient nutrient access, making it possible to produce large-scale tumor and normal tissue models in vitro, while providing insights into drug efficacy and toxicology evaluation as well as standardized organoid production.

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On-line Access: 2025-05-20

Received: 2024-10-20

Revision Accepted: 2024-12-02

Crosschecked: 0000-00-00

Cited: 0

Clicked: 1024

Citations: Bibtex RefMan EndNote GB/T7714

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